The condensed protocols from molecular cloning : a laboratory manual

フォーマット:

図書

責任表示:

Joseph Sambrook, David W. Russell

言語:

英語

出版情報:

Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006

形態:

v, 800 p. ; 29 cm

著者名:

• Sambrook, Joseph <DA00594459>
• Russell, David W. <DA1424172X>

目次情報:

Plasmids and their usefulness in molecular cloning
Bacteriophage λ and its vectors
Working with bacteriophage M13 vectors
Working with high-capacity vectors
Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
Preparation and analysis of eukaryotic genomic DNA
Extraction, purification, and analysis of mRNA from eukaryotic cells
In vitro amplification of DNA by the polymerase chain reaction
Preparation of radiolabeled DNA and RNA probes
Working with synthetic oligonucleotide probes
Preparation of cDNA libraries and gene identification
DNA sequencing
Mutagenesis
Screening expression libraries
Expression of cloned genes in escherichia coli
Introducing cloned genes into cultured mammalian cells
Analysis of gene expression in mammalian cells
Protein interaction technologies

Plasmids and their usefulness in molecular cloning
Bacteriophage λ and its vectors
Working with bacteriophage M13 vectors
Working with high-capacity vectors
Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
Preparation and analysis of eukaryotic genomic DNA

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書誌ID:

BA77594444

ISBN:

9780879697723 [0879697725] (: hardcover)      
9780879697716 [0879697717] (: pbk)