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The CD45+ fraction in murine adipose tissue derived stromal cells harbors immune-inhibitory inflammatory cells

フォーマット:
論文
責任表示:
Nasti, Alessandro ; Sakai, Yoshio ; Seki, Akihiro ; Buffa, Geraldine Belen ; Komura, Takuya ; Mochida, Hatsune ; Yamato, Masatoshi ; Yoshida, Keiko ; Ho, Tuyen T. B. ; Takamura, Masayuki ; Usui, Soichiro ; Wada, Takashi ; Honda, Masao ; Kaneko, Shuichi ; 酒井, 佳夫 ; 餅田, 初音 ; 吉田, 佳子 ; 高村, 雅之 ; 薄井, 荘一郎 ; 和田, 隆志 ; 本多, 政夫 ; 金子, 周一
言語:
英語
出版情報:
Wiley, 2017-12
著者名:
Nasti, Alessandro
Sakai, Yoshio
Seki, Akihiro
Buffa, Geraldine Belen
Komura, Takuya
Mochida, Hatsune
Yamato, Masatoshi
Yoshida, Keiko
Ho, Tuyen T. B.
Takamura, Masayuki
Usui, Soichiro
Wada, Takashi
Honda, Masao
Kaneko, Shuichi
酒井, 佳夫
餅田, 初音
吉田, 佳子
高村, 雅之
薄井, 荘一郎
和田, 隆志
本多, 政夫
金子, 周一
続きを見る
掲載情報:
European Journal of Immunology
ISSN:
0014-2980  CiNii Research  Webcat Plus  JAIRO
巻:
47
通号:
12
開始ページ:
2163
終了ページ:
2174
バージョン:
author
概要:
金沢大学医薬保健研究域医学系<br />Stromal cells in adipose tissue are useful for repair/regenerative therapy as they harbor a substantial number of mesenchymal stem cells; therefore, freshly isolated autologous uncultured adipose tissue derived str omal cells (u-ADSCs) are useful for regenerative therapy, and obviate the need for mesenchymal stem cells. We evaluated the therapeutic effect of murine u-ADSCs and sorted subsets of u-ADSCs in a concanavalin A (ConA) induced murine model of hepatitis, as well as their characteristics. We found that 10–20% of u-ADSCs expressed the CD45 leukocyte-related antigen. CD68, which is a marker of macrophages (MΦs), was expressed by 50% of CD45+ u-ADSCs. About 90% of CD68+CD45+ cells expressed CD206 antigen, which is a marker of inhibitory M2-type MΦs. Genes related to M2-type MUs were especially more highly expressed by CD45+CD206+ u-ADSCs than by CD45− u-ADSCs. CD45+ u-ADSCs inhibited the expression of cytokines/chemokines and suppressed the proliferation of splenocytes stimulated with ConA. We observed that not only whole u-ADSCs, but also the CD45+ subset of u-ADSCs ameliorated the ConA-induced hepatitis in mice. In conclusion, we show that freshly isolated murine u-ADSCs were effective against acute hepatitis, and CD45+ u-ADSCs acting phenotypically and functionally like M2-type MΦs, contributed to the repair of liver tissue undergoing inflammation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim<br />Embargo Period 12 months 続きを見る
URL:
http://hdl.handle.net/2297/00050487
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