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MTMR4, a phosphoinositide-specific 3'-phosphatase, regulates TFEB activity and the endocytic and autophagic pathways.
- フォーマット:
- 論文
- 責任表示:
- Pham, Hoa Q. ; Yoshioka, Kazuaki ; Mohri, Hiromi ; Nakata, Hiroki ; Aki, Sho ; Ishimaru, Kazuhiro ; Takuwa, Noriko ; Takuwa, Yoh ; 吉岡, 和晃 ; 仲田, 浩規 ; 安藝, 翔 ; 多久和, 典子 ; 多久和, 陽
- 言語:
- 英語
- 出版情報:
- John Wiley & Sons, 2018-08
- 著者名:
Pham, Hoa Q. Yoshioka, Kazuaki Mohri, Hiromi Nakata, Hiroki Aki, Sho Ishimaru, Kazuhiro Takuwa, Noriko Takuwa, Yoh 吉岡, 和晃 仲田, 浩規 安藝, 翔 多久和, 典子 多久和, 陽 - 掲載情報:
- Genes Cells
- ISSN:
- 1356-9597
- 巻:
- 23
- 通号:
- 8
- 開始ページ:
- 670
- 終了ページ:
- 687
- バージョン:
- author
- 概要:
- 金沢大学医薬保健研究域医学系<br />Phosphatidylinositol 3-phosphate (PI(3)P) is the predominant phosphoinositide species in early endosomes and autophagosomes, in which PI(3)P dictates traffic of these organelles. Phosphoinositide levels are tightly … regulated by lipid-kinases and -phosphatases; however, a phosphatase that converts PI(3)P back to phosphatidylinositol in the endosomal and autophagosomal compartments is not fully understood. We investigated the subcellular distribution and functions of myotubularin-related protein-4 (MTMR4), which is distinct among other MTMRs in that it possesses a PI(3)P-binding FYVE domain, in lung alveolar epithelium-derived A549 cells. MTMR4 was localized mainly in late endosomes and autophagosomes. MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P-enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P-enriched early and late endosomes. In amino acid- and serum-starved cells, MTMR4 knockdown decreased both autophagosomes and autolysosomes and markedly increased PI(3)P-containing autophagosomes and late endosomes, suggesting that the fusion with lysosomes of autophagosomes and late endosomes might be impaired. Notably, MTMR4 knockdown inhibited the nuclear translocation of starvation stress responsive transcription factor-EB (TFEB) with reduced expression of lysosome-related genes in starved cells. These findings indicate that MTMR4 is essential for the integrity of endocytic and autophagic pathways. © 2018 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.<br />Embargo Period 12 months 続きを見る
- URL:
- http://hdl.handle.net/2297/00053882
類似資料:
Physiological Society of Japan 日本生理学会 / Springer Nature | |
Endocrine Society / Oxford University Press |
|
Public Library of Science | |
金沢大学十全医学会 = The Juzen Medical Society Kanazawa University | |
Japan Society of Smooth Muscle Research = 日本平滑筋学会 | |