RNA interference silencing of DRAL affects processing of amyloid precursor protein

フォーマット:

論文

責任表示:

Tanahashi, Hiroshi ; Yoshioka, Kazuaki

言語:

英語

出版情報:

Elsevier, 2008-07-18

著者名:

• Tanahashi, Hiroshi
• Yoshioka, Kazuaki

掲載情報:

Neuroscience Letters

ISSN:

0304-3940      

巻:

439

通号:

3

開始ページ:

293

終了ページ:

297

バージョン:

author

概要:

金沢大学医薬保健研究域医学系<br />In a previous study, we reported that Alzheimer's disease-associated presenilin-2 interacts with a LIM-domain protein, namely, DRAL/FHL2/SLIM3. In this study, we investigated whether DRAL modifies the metaboli … sm of the amyloid precursor protein (APP). We used small interfering RNA (siRNA) to knockdown DRAL in COS7 and HEK293 cells that stably overexpress APP695. We found that the knockdown was accompanied by a decrease in the amount of secreted α-secretase-cleaved APP and the membrane-bound C-terminal fragment C83 and an increase in the amount of secreted β-amyloid peptide (Aβ) from the cells. We also found that in addition to a disintegrin and metalloprotease (ADAM)-17, DRAL binds to ADAM-10. Thus, DRAL may be involved in the processing of APP through the α-secretase pathway. © 2008 Elsevier Ireland Ltd. All rights reserved. 続きを見る

URL:

http://hdl.handle.net/2297/10014