Cytosolic Ca2+ alteration mediates both ryanodine receptor and IP3 receptor in TE671/RD cells

フォーマット:

論文

責任表示:

Edahiro, Shigeki ; Yoshikawa, Hiroaki ; Iwasa, Kazuo ; Hashii, Minako ; Yamada, Masahito

言語:

英語

出版情報:

Biomedical Research, 2004-12-01

著者名:

• Edahiro, Shigeki
• Yoshikawa, Hiroaki
• Iwasa, Kazuo
• Hashii, Minako
• Yamada, Masahito

掲載情報:

Biomedical Research

ISSN:

0388-6107      

巻:

25

通号:

6

開始ページ:

255

終了ページ:

261

バージョン:

publisher

概要:

金沢大学医薬保健研究域医学系<br />TE671 is a cell line obtained from the rhabdomyosarcoma cells. In the present study, we examined [Ca2+]i alteration induced by acetylcholine (ACh) in TE671/RD cells with the special attention to ryanodine receptor … (RyR) and inositol 1,4,5-triphosphate receptor (IP3R). The change of [Ca2+]i was mediated by muscarinic type 3 (m3) AChR. Both phosphatidylinositol-specific phospholipase C blocker (U73122) and IP3R blocker (2-APB) inhibited ACh-induced [Ca2+]i elevation, suggesting that IP3 pathway is involved in [Ca2+]i alteration. Ryanodine and FK506 increased ACh-induced [Ca2+]i elevation, which was decreased by RyR blocker (ruthenium red). These results suggest that RyR and FK506 binding protein 12 kDa (FKBP12) complex was involved in [Ca2+]i change, and that TE671/RD cell line has a hybrid characteristic of smooth and skeletal muscles. Copyright © 2005 Biomedical Research Press. 続きを見る

URL:

http://hdl.handle.net/2297/14432