Absolute determination method for trace quantities of enantiomer of glufosinate by γ-cyclodextrin modified capillary zone electrophoresis combined with solid-phase extraction and on-capillary concentration

フォーマット:

論文

責任表示:

Asami, Takayuki ; Imura, Hisanori

言語:

英語

出版情報:

日本分析化学会 = Japan Society for Analytical Chemistry, 2006-12-01

著者名:

• Asami, Takayuki
• Imura, Hisanori

掲載情報:

Analytical Sciences

ISSN:

0910-6340      

巻:

22

通号:

12

開始ページ:

1489

終了ページ:

1493

バージョン:

publisher

概要:

金沢大学理工研究域物質化学系<br />A highly sensitive and accurate determination method for trace quantities of enantiomers of glufosinate (D,L-GLUF), a phosphorus-containing amino acid-type herbicide, has been studied. The present method is based o … n the change in the mole ratio of the enantiomeric isomers after spiking of a known amount of an isomer (L-GLUF). The chiral separation and detection were made by γ-cyclodextrin modified capillary zone electrophoresis (γ-CD-CZE) with fluorescence detection. Solid-phase extraction of D,L-GLUF with titania was investigated as the pre-separation method to eliminate coexisting materials such as inorganic salts and organic compounds in river water. A separated D,L-GLUF was labeled with dansyl chloride and subjected to the on-capillary concentration using large-volume sample stacking (LVSS) before γ-CD-CZE. The detection limit of the present method was as low as 2.0 × 10 -9 M. The present method was successfully applied to a model sample containing 2.0 × 10-7 M D,L-GLUF in river water. It was confirmed that trace quantities of D-and L-GLUF in environmental samples can be accurately determined without any calibration curves and comparison standards. 2006 © The Japan Society for Analytical Chemistry. 続きを見る

URL:

http://hdl.handle.net/2297/14501