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Mutational analysis of human RNA polymerase II subunit 5 (RPB5): The residues critical for interactions with TFIIF subunit RAP30 and hepatitis B virus X protein

フォーマット:
論文
責任表示:
Le, Thi Thu Thuy ; Zhang, Shijun ; Hayashi, Naoyuki ; Yasukawa, Mami ; Delgermaa, Luvsanjav ; Murakami, Seishi
言語:
英語
出版情報:
日本生化学会 = Japanese Biochemical Society, 2005-09-01
著者名:
Le, Thi Thu Thuy
Zhang, Shijun
Hayashi, Naoyuki
Yasukawa, Mami
Delgermaa, Luvsanjav
Murakami, Seishi
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掲載情報:
Journal of Biochemistry
ISSN:
0021-924x  CiNii Research  Webcat Plus  JAIRO
巻:
138
通号:
3
開始ページ:
215
終了ページ:
224
バージョン:
publisher
概要:
金沢大学がん研究所<br />RNA polymerase II (RNAPII) subunit 5 (RPB5) is positioned close to DNA downstream of the initiation site and is the site of interaction with several regulators. Hepatitis B virus X protein (HBx) binds the central part o f RPB5 to modulate activated transcription, and TFIIF subunit RAP30 interacts with the same part of RPB5 that is critical for the association between TFIIF and RNAPII. However the residues necessary for these interactions remain unknown. Here we report systematic mutagenesis of the central part of RPB5 using two-step alanine scanning libraries to pinpoint critical residues for its binding to RAP30 in the TFIIF complex and/or to HBx, and identified these residues in both mammalian cells and in an in vitro binding assay. Four residues, F76, I104, T111 and S113, are critical for both TFIIF- and HBx-binding, indicating the overlapping nature of the sites of interaction. In addition, V74 and N98 are required for HBx-binding, and T56 and L58 are needed for RAP30-binding. Interestingly the residues exposed to solvent, T111 and S113, are very close to the DNA, implying that two factors may modulate the interaction between DNA and RPB5. © 2005 The Japanese Biochemical Society. 続きを見る
URL:
http://hdl.handle.net/2297/14550
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