Independence of plasma membrane blebbing from other biochemical and biological characteristics of apoptotic cells

フォーマット:

論文

責任表示:

Shiratsuchi, Akiko ; Mori, Tomoe ; Nakanishi, Yoshinobu

言語:

英語

出版情報:

日本生化学会 = Japanese Biochemical Society, 2002-09-01

著者名:

• Shiratsuchi, Akiko
• Mori, Tomoe
• Nakanishi, Yoshinobu

掲載情報:

Journal of Biochemistry

ISSN:

0021-924x      

巻:

132

通号:

3

開始ページ:

381

終了ページ:

386

バージョン:

publisher

概要:

金沢大学医薬保健研究域薬学系<br />Plasma membrane blebs are observed in many types of apoptotic cells, but their physiological roles remain to be clarified. We examined whether there is a causative connection between membrane blebbing and other apo … ptotic changes in Jurkat cells induced to undergo apoptosis by doxorubicin in the presence or absence of Y-27632, an inhibitor of the Rho kinase ROCK-I. The inclusion of the drug made most membrane blebs disappear, while other changes, such as chromatin condensation, inactivation of mitochondrial enzymes, externalization of the membrane phospholipid phosphatidylserine, and removal of cell surface sialic acid, remained unaffected. Furthermore, these apoptotic cells were phagocytosed by macrophages as efficiently as normally apoptosing cells. These results indicate that blebbing of the plasma membrane occurs independently from other apoptotic changes and is not involved in the recognition and engulfment of apoptotic cells by macrophages. 続きを見る

URL:

http://hdl.handle.net/2297/14555