Determination of 1-nitropyrene in low volume ambient air samples by high-performance liquid chromatography with fluorescence detection

フォーマット:

論文

責任表示:

Ohno, Takanori ; Toriba, Akira ; Kameda, Takayuki ; Tang, Ning ; Hayakawa, Kazuichi

言語:

英語

出版情報:

Elsevier BV, 2009-05-22

著者名:

• Ohno, Takanori
• Toriba, Akira
• Kameda, Takayuki
• Tang, Ning
• Hayakawa, Kazuichi

掲載情報:

Journal of Chromatography A

ISSN:

0021-9673      

巻:

1216

通号:

21

開始ページ:

4625

終了ページ:

4628

バージョン:

author

概要:

金沢大学医薬保健研究域薬学系<br />To measure the actual exposure of a person to 1-nitropyrene (1-NP) in airborne particulate matter, it is considered more accurate to collect air samples with a portable air sampler than to sample at a fixed locatio … n. However, because the portable samplers can sample only small volumes, a sensitive method is needed to analyze the compounds that are collected on a filter. Here we describe a high-performance liquid chromatographic (HPLC) method with fluorescence detection that is sensitive and precise enough for use with portable air samplers. The developed column-switching system successfully removed the interfering substances in the samples with only a simple pretreatment. To improve the precision of the measurement, deuterated 1-NP was used as an internal standard, and it eluted immediately prior to 1-NP with sufficient resolution (Rs, 1.50). The detection limit was 0.32 fmol/injection, and the calibration range was from 2 to 100 fmol. The proposed method was applied to determining 1-NP in fine airborne particulate matter (PM2.5) at two sites with low pollution levels. 1-NP was detected in all samples at concentrations in the low fmol/m3 range. The proposed method has enough sensitivity and precision to determine 1-NP in the limited air volume of the portable sampler. © 2009 Elsevier B.V. All rights reserved. 続きを見る

URL:

http://hdl.handle.net/2297/17576