Nesfatin-1 evokes Ca2+ signaling in isolated vagal afferent neurons via Ca2+ influx through N-type channels

フォーマット:

論文

責任表示:

Iwasaki, Yusaku ; Nakabayashi, Hajime ; Kakei, Masafumi ; Shimizu, Hiroyuki ; Mori, Masatomo ; Yada, Toshihiko

言語:

英語

出版情報:

Elsevier, 2009-12-18

著者名:

Iwasaki, Yusaku

Nakabayashi, Hajime

Kakei, Masafumi

Shimizu, Hiroyuki

Mori, Masatomo

Yada, Toshihiko

続きを見る

掲載情報:

Biochemical and Biophysical Research Communications

ISSN:

0006-291x      

巻:

390

通号:

3

開始ページ:

958

終了ページ:

962

バージョン:

author

概要:

金沢大学医薬保健研究域医学系<br />Nesfatin-1, processed from nucleobindin 2, is an anorexigenic peptide expressed in the brain and several peripheral tissues including the stomach and pancreas. Peripheral, as well as intracerebroventricular, admini … stration of nesfatin-1 suppresses feeding behavior, though underlying mechanisms are unknown. In this study, we examined effects of nesfatin-1 on cytosolic Ca2+ concentration ([Ca2+]i) in the neurons isolated from the vagal afferent nodose ganglion of mice. Nesfatin-1 at 10-10-10-8 M increased [Ca2+]i in the isolated neurons in a concentration-dependent manner, and at 10-8 M it increased [Ca2+]i in 33 out of 263 (12.5%) neurons. These responses were inhibited under Ca2+-free conditions and by N-type Ca2+ channel blocker, ω-conotoxin GVIA. All the nesfatin-1-responsive neurons also exhibited [Ca2+]i responses to capsaicin and cholecystokinin-8. These results provide direct evidence that nesfatin-1 activates vagal afferent neurons by stimulating Ca2+ influx through N-type channels, demonstrating the machinery through which peripheral nesfatin-1 can convey signals to the brain. © 2009 Elsevier Inc. All rights reserved. 続きを見る

URL:

http://hdl.handle.net/2297/20509