NIRS measurement of O(2) dynamics in contracting blood and buffer perfused hindlimb muscle.

フォーマット:

論文

責任表示:

Masuda, Kazumi ; Takakura, Hisashi ; Furuichi, Yasuro ; Iwase, Satoshi ; Jue, Thomas

言語:

英語

出版情報:

Kluwer (now part of Springer), 2010-01-01

著者名:

• Masuda, Kazumi
• Takakura, Hisashi
• Furuichi, Yasuro
• Iwase, Satoshi
• Jue, Thomas

掲載情報:

Advances in experimental medicine and biology

ISSN:

0065-2598      

巻:

662

開始ページ:

323

終了ページ:

328

バージョン:

author

概要:

金沢大学人間社会研究域人間科学系<br />In order to obtain evidence that Mb releases O(2) during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. … The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (Delta[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of Delta[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the Delta[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO(2) as workload increases. 続きを見る

URL:

http://hdl.handle.net/2297/23921