Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier

Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier

フォーマット:

論文

責任表示:

Iwanaga, Atsushi ; Asakawa, Hitoshi ; Fukuma, Takeshi ; Nakamichi, Momoka ; Shigematsu, Sakurako ; Linder, Markus B. ; Haruyama, Tetsuya

言語:

英語

出版情報:

Elsevier B.V., 2011-06-01

著者名:

掲載情報:

Colloids and Surfaces B: Biointerfaces

ISSN:

09277765

巻:

84

通号:

2

開始ページ:

395

終了ページ:

399

バージョン:

author

概要:

金沢大学理工研究域バイオＡＦＭ先端研究センター<br />A groundbreaking method for ordered molecular layer preparation on a solid surface employing the drop-stamp method has been developed by us taking advantage of the characteristics of the HFB molecule as a … self-organizer/adsorption carrier. It is a smart method which can be used to prepare a self-organized protein layer on a solid surface without unspecific adsorption or defects. In our previous report, we clarified the self-organizing nature of HFB-tagged protein molecules on a surface of a solution droplet. In this report, a protein layer was prepared on a HOPG surface by using the drop-stamp method with a maltose binding protein (MBP)-tagged HFBII molecule. The structure of the stamped protein layer was investigated using frequency modulation atomic force microscopy (FM-AFM) in a liquid condition. The FM-AFM images show that the drop-stamp method can prepare an ordered protein layer on a solid surface smartly. The drop-stamp method using a HFB carrier is a practical method which can be used to prepare an ordered protein layer on a solid substrate surface without unspecific adsorption defects. © 2011 Elsevier B.V. 続きを見る

URL:

http://hdl.handle.net/2297/27695

タイトル・著者・出版者が同じ資料

詳細

主題:	Drop-stamp; Hydrophobin; Molecular interface; Self-organized protein layer
受理日:	2011-06-01

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