Identification of a secretory protein c19orf10 activated in hepatocellular carcinoma

フォーマット:

論文

責任表示:

Sunagozaka, Hajime ; Honda, Masao ; Yamashita, Taro ; Nishino, Ryuhei ; Takatori, Hajime ; Arai, Kuniaki ; Yamashita, Tatsuya ; Sakai, Yoshio ; Kaneko, Shuichi

言語:

英語

出版情報:

Wiley-Blackwell, 2011-10-01

著者名:

Sunagozaka, Hajime

Honda, Masao

Yamashita, Taro

Nishino, Ryuhei

Takatori, Hajime

Arai, Kuniaki

Yamashita, Tatsuya

Sakai, Yoshio

Kaneko, Shuichi

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掲載情報:

International Journal of Cancer

ISSN:

0020-7136      

巻:

129

通号:

7

開始ページ:

1576

終了ページ:

1585

バージョン:

author

概要:

金沢大学医薬保健研究域医学系<br />The identification of genes involved in tumor growth is crucial for the development of inventive anticancer treatments. Here, we have cloned a 17-kDa secretory protein encoded by c19orf10 from hepatocellular carcin … oma (HCC) serial analysis of gene expression libraries. Gene expression analysis indicated that c19orf10 was overexpressed in approximately two-thirds of HCC tissues compared to the adjacent noncancerous liver tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP). Overexpression of c19orf10 enhanced cell proliferation of AFP-negative HLE cells, whereas knockdown of c19orf10 inhibited cell proliferation of AFP-positive Hep3B and HuH7 cells along with G1 cell cycle arrest. Supplementation of recombinant c19orf10 protein in culture media enhanced cell proliferation in HLE cells, and this effect was abolished by the addition of antibodies developed against c19orf10. Intriguingly, c19orf10 could regulate cell proliferation through the activation of Akt/mitogen-activated protein kinase pathways. Taken together, these data suggest that c19orf10 might be one of the growth factors and potential molecular targets activated in HCC. © 2010 UICC. 続きを見る

URL:

http://hdl.handle.net/2297/28230