高速原子間力顕微鏡によるタンパク質の動態撮影

フォーマット:

論文

責任表示:

安藤, 敏夫 ; 古寺, 哲幸

言語:

日本語

出版情報:

日本生物物理学会 = The Biophysical Society of Japan, 2011-01-01

著者名:

• 安藤, 敏夫
• 古寺, 哲幸

掲載情報:

生物物理 = Biophys

ISSN:

1099-5404      

巻:

51

通号:

1

開始ページ:

022

終了ページ:

025

バージョン:

publisher

概要:

Proteins are dynamic in nature and work at the single-molecule level. Reflecting this fact, single-molecule fluorescence microscopy has been widely exploited to understand how proteins operate. However, what we can observe thereby is the dynamic behaviour of individual fluorescent spots (each being emitted from a fluorophore attached to a selected locus of the molecule), not of the protein molecules themselves. The structure of proteins has been studied by electron … microscopy, X-ray crystallography and NMR, but the obtained structures are essentially static. This long-standing problem prevailing throughout biological research has been recently overcome by the development of high-speed atomic force microscopy that enables simultaneous recording of the structure and dynamics of functioning biomolecules with high spatiotemporal resolution. 続きを見る

URL:

http://hdl.handle.net/2297/28482