Apoptosis-dependent externalization and involvement in apoptotic cell clearance of DmCaBP1, an endoplasmic reticulum protein of Drosophila

Apoptosis-dependent externalization and involvement in apoptotic cell clearance of DmCaBP1, an endoplasmic reticulum protein of Drosophila

フォーマット:

論文

責任表示:

Okada, Ryo ; Nagaosa, Kazushige ; Kuraishi, Takayuki ; Nakayama, Hiroshi ; Yamamoto, Naoko ; Nakagawa, Yukiko ; Dohmae, Naoshi ; Shiratsuchi, Akiko ; Nakanishi, Yoshinobu

言語:

英語

出版情報:

American Society for Biochemistry and Molecular Biology, 2012-01-27

著者名:

掲載情報:

Journal of Biological Chemistry

ISSN:

0021-9258

巻:

287

通号:

5

開始ページ:

3138

終了ページ:

3146

バージョン:

author

概要:

To elucidate the actions of Draper, a receptor responsible for the phagocytic clearance of apoptotic cells in Drosophila, we isolated proteins that bind to the extracellular region of Draper using affinity chromatography. One of those proteins has been identified to be an uncharacterized protein called Drosophila melanogaster calcium-binding protein 1 (DmCaBP1). This protein containing the thioredoxin-like domain resided in the endoplasmic reticulum and seemed to be … expressed ubiquitously throughout the development of Drosophila. DmCaBP1 was externalized without truncation after the induction of apoptosis somewhat prior to chromatin condensation and DNA cleavage in a manner dependent on the activity of caspases. A recombinant DmCaBP1 protein bound to both apoptotic cells and a hemocyte-derived cell line expressing Draper. Forced expression of DmCaBP1 at the cell surface made non-apoptotic cells susceptible to phagocytosis. Flies deficient in DmCaBP1 expression developed normally and showed Draper-mediated pruning of larval axons, but a defect in the phagocytosis of apoptotic cells in embryos was observed. Loss of Pretaporter, a previously identified ligand for Draper, did not cause a further decrease in the level of phagocytosis in DmCaBP1-lacking embryos. These results collectively suggest that the endoplasmic reticulum protein DmCaBP1 is externalized upon the induction of apoptosis and serves as a tethering molecule to connect apoptotic cells and phagocytes for effective phagocytosis to occur. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. 続きを見る

URL:

http://hdl.handle.net/2297/30324

タイトル・著者・出版者が同じ資料

詳細

受理日:	2012-01-27

類似資料:

1

2

3

4

5

6

7

8

9

10

11

12

1 論文 Pretaporter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells Kuraishi, Takayuki, Nakagawa, Yukiko, Nagaosa, Kazushige, Hashimoto, Yumi, Ishimoto, Takashi, Moki, Takeshi, Fujita, Yu, … Nature Publishing Group	7 論文 Phosphatidylserine-mediated phagocytosis of anticancer drug-treated cells by macrophages Shiratsuchi, Akiko, Nakanishi, Yoshinobu 日本生化学会 = Japanese Biochemical Society
2 論文 Phagocytic removal of cells that have become unwanted: Implications for animal development and tissue homeostasis Nakanishi, Yoshinobu, Nagaosa, Kazushige, Shiratsuchi, Akiko Wiley-Blackwell / 日本発生生物学会 = Japanese Society of Developmental Biologists (JSDB)	8 論文 Isolation of a Drosophila gene coding for a protein containing a novel phosphatidylserine-binding motif Nakai, Yuji, Nomura, Yoshitaka, Sato, Toshihiro, Shiratsuchi, Akiko, Nakanishi, Yoshinobu 日本生化学会 = Japanese Biochemical Society
3 論文 Structural change of ribosomes during apoptosis: Degradation and externalization of ribosomal proteins in doxorubicin-treated Jurkat cells Nishida, Jun, Shiratsuchi, Akiko, Nadano, Daita, Sato, Takaaki, Nakanishi, Yoshinobu 日本生化学会 = Japanese Biochemical Society	9 論文 Signalling pathway involving GULP, MAPK and Rac1 for SR-BI-induced phagocytosis of apoptotic cells. Osada, Yoichi, Sunatani, Toshiro, Kim, In-San, Nakanishi, Yoshinobu, Shiratsuchi, Akiko 日本生化学会 = Japanese Biochemical Society
4 論文 Peptidoglycan recognition protein-triggered induction of Escherichia coli gene in Drosophila melanogaster Kong, Qingquan, Nakai, Yuji, Kuroda, Nanae, Shiratsuchi, Akiko, Nagaosa, Kaz, Nakanishi, Yoshinobu Japanese Biochemical Society = 日本生化学会 / Oxford University Press	10 論文 A presumed human nuclear autoantigen that translocates to plasma membrane blebs during apoptosis Shiratsuchi, Akiko, Mori, Tomoe, Takahashi, Yae, Sakai, Koichiro, Nakanishi, Yoshinobu 日本生化学会 = Japanese Biochemical Society
5 論文 Signaling pathway for phagocyte priming upon encounter with apoptotic cells Nonaka, Saori, Ando, Yuki, Kanetani, Takuto, Hoshi, Chiharu, Nakai, Yuji, Nainu, Firzan, Nagaosa, Kaz, Shiratsuchi, … American Society for Biochemistry and Molecular Biology Inc.	11 論文 Induction of apoptosis and subsequent phagocytosis of virus-infected cells as an antiviral mechanism Nainu, Firzan, Shiratsuchi, Akiko, Nakanishi, Yoshinobu, 白土, 明子, 中西, 義信 Frontiers Media S.A.
6 論文 Independence of plasma membrane blebbing from other biochemical and biological characteristics of apoptotic cells Shiratsuchi, Akiko, Mori, Tomoe, Nakanishi, Yoshinobu 日本生化学会 = Japanese Biochemical Society	12 その他 Study on the mechanism of phagocyte migration to apoptotic cells : Analysis of the induction of apoptosis and MCP-1 expression in regressive corpora lutea of … 永長, 一茂, Nagaosa, Kazushige 金沢大学