Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma

Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma

フォーマット:

論文

責任表示:

Marukawa, Yohei ; Nakamoto, Yasunari ; Kakinoki, Kaheita ; Tsuchiyama, Tomoya ; Iida, Noriho ; Kagaya, Takashi ; Sakai, Yoshio ; Naito, Makoto ; Mukaida, Naofumi ; Kaneko, Shuichi

言語:

英語

出版情報:

Nature Publishing Group, 2012-05-01

著者名:

掲載情報:

Cancer Gene Therapy

ISSN:

0929-1903

巻:

19

通号:

5

開始ページ:

312

終了ページ:

319

バージョン:

author

概要:

Suicide gene therapy using the herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system combined with monocyte chemoattractant protein-1 (MCP-1) provides significant antitumor efficacy. The current study was designed to evaluate the antitumor immunity of a newly developed membrane-bound form of MCP-1 (mMCP-1) in an immunocompetent mouse model of hepatocellular carcinoma (HCC). A recombinant adenovirus vector (rAd) harboring the human MCP-1 gene and … the membrane-spanning domain of the CX3CL1 gene was used. Large amounts of MCP-1 protein were expressed and accumulated on the tumor cell surface. The growth of subcutaneous tumors was markedly suppressed when tumors were treated with mMCP-1, as compared with soluble MCP-1, in combination with the HSV-tk/GCV system (P<0.01). The numbers of Mac-1-, CD4- and CD8a-positive cells were significantly higher in tumor tissues (P<0.05), and tumor necrosis factor (TNF) mRNA expression levels with mMCP-1 were almost five-fold higher than those with soluble MCP-1. These results indicate that the delivery of the mMCP-1 gene greatly enhanced antitumor effects following the apoptotic stimuli by promoting the recruitment and activation of macrophages and T lymphocytes, suggesting a novel strategy of immune-based gene therapy in the treatment of patients with HCC.Cancer Gene Therapy advance online publication, 9 March 2012; doi:10.1038/cgt.2012.3. 続きを見る

URL:

http://hdl.handle.net/2297/30373

タイトル・著者・出版者が同じ資料

詳細

受理日:	2012-05-01

類似資料:

1

2

3

4

5

6

7

8

9

10

11

12

1 論文 Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma Marukawa, Yohei, Nakamoto, Yasunari, Kakinoki, Kaheita, Tsuchiyama, Tomoya, Iida, Noriho, Kagaya, Takashi, Sakai, … Nature Publishing Group	7 論文 Tumor cell apoptosis induces tumor-specific immunity in a CC chemokine receptor 1- and 5-dependent manner in mice Iida, Noriho, Nakamoto, Yasunari, Baba, Tomohisa, Kakinoki, Kaheita, Li, Ying-Yi, Wu, Yu, Matsushita, Kouji, Kaneko, … Oxford University Press
2 論文 Optimal amount of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy against hepatocellular carcinoma by M1 macrophage … Tsuchiyama, Tomoya, Nakamoto, Yasunari, Sakai, Yoshio, Mukaida, Naofumi, Kaneko, Shuichi Japanese Cancer Association / Blackwell Publishing Ltd	8 論文 Frequency of CD45RO+ subset in CD4+CD25high regulatory T cells associated with progression of hepatocellular carcinoma Takata, Yoshiko, Nakamoto, Yasunari, Nakada, Akiko, Terashima, Takeshi, Arihara, Fumitaka, Kitahara, Masaaki, Kakinoki, … Elsevier
3 論文 Prevention of intrahepatic metastasis of liver cancer by suicide gene therapy and chemokine ligand 2/monocyte chemoattractant protein-1 delivery in mice Kakinoki, Kaheita, Nakamoto, Yasunari, Kagaya, Takashi, Tsuchiyama, Tomoya, Sakai, Yoshio, Nakahama, Tohru, Mukaida, … Wiley-Blackwell	9 論文 In vivo immunological antitumor effect of OK-432-stimulated dendritic cell transfer after radiofrequency ablation Nakagawa, Hidetoshi, Mizukoshi, Eishiro, Iida, Noriho, Terashima, Takeshi, Kitahara, Masaaki, Marukawa, Yohei, Kitamura, … Springer-Verlag
4 論文 Prolonged, NK cell-mediated antitumor effects of suicide gene therapy combined with monocyte chemoattractant protein-1 against hepatocellular carcinoma. Tsuchiyama, T., Nakamoto, Yasunari, Sakai, Y., Mukaida, Naofumi, Sawabu, Norio, Kaneko, Shuichi 金沢大学がん研究所	10 論文 Efficient generation of highly immunocompetent dendritic cells from peripheral blood of patients with hepatitis C virus-related hepatocellular carcinoma Kitahara, Masaaki, Mizukoshi, Eishiro, Nakamoto, Yasunari, Mukaida, Naofumi, Matsushima, Kouji, Kaneko, Shuichi Elsevier
5 論文 Prolonged recurrence-free survival following OK432-stimulated dendritic cell transfer into hepatocellular carcinoma during transarterial embolization Nakamoto, Yasunari, Mizukoshi, Eishiro, Kitahara, Masaaki, Arihara, Fumitaka, Sakai, Yoshio, Kakinoki, Kaheita, Fujita, … Blackwell Publishing	11 論文 Aberrant expression of serine/threonine kinase Pim-3 in hepatocellular carcinoma development and its role in the proliferation of human hepatoma cell lines Fujii, Chifumi, Nakamoto, Yasunari, Lu, Peirong, Tsuneyama, Koichi, Popivanova, Boryana K., Kaneko, Shuichi, Mukaida, … Wiley-Liss
6 論文 Antitumor effect after radiofrequency ablation of murine hepatoma is augmented by an active variant of CC chemokine ligand 3/macrophage inflammatory protein-1á Iida, Noriho, Nakamoto, Yasunari, Baba, Tomohisa, Nakagawa, Hidetoshi, Mizukoshi, Eishiro, Naito, Makoto, Mukaida, … American Association for Cancer Research	12 その他 Enhanced antitumor effects of a bicistronic adenovirus vector expressing both herpes simplex virus thymidine kinase and monocyte chemoattractant protein-1 … Tsuchiyama, Tomoya, 土山, 智也金沢大学