Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma

Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma

フォーマット:

論文

責任表示:

Marukawa, Yohei ; Nakamoto, Yasunari ; Kakinoki, Kaheita ; Tsuchiyama, Tomoya ; Iida, Noriho ; Kagaya, Takashi ; Sakai, Yoshio ; Naito, Makoto ; Mukaida, Naofumi ; Kaneko, Shuichi

言語:

英語

出版情報:

Nature Publishing Group, 2012-05-01

著者名:

掲載情報:

Cancer Gene Therapy

ISSN:

0929-1903

巻:

19

通号:

5

開始ページ:

312

終了ページ:

319

バージョン:

author

概要:

Suicide gene therapy using the herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system combined with monocyte chemoattractant protein-1 (MCP-1) provides significant antitumor efficacy. The current study was designed to evaluate the antitumor immunity of a newly developed membrane-bound form of MCP-1 (mMCP-1) in an immunocompetent mouse model of hepatocellular carcinoma (HCC). A recombinant adenovirus vector (rAd) harboring the human MCP-1 gene and … the membrane-spanning domain of the CX3CL1 gene was used. Large amounts of MCP-1 protein were expressed and accumulated on the tumor cell surface. The growth of subcutaneous tumors was markedly suppressed when tumors were treated with mMCP-1, as compared with soluble MCP-1, in combination with the HSV-tk/GCV system (P<0.01). The numbers of Mac-1-, CD4-and CD8a-positive cells were significantly higher in tumor tissues (P<0.05), and tumor necrosis factor (TNF) mRNA expression levels with mMCP-1 were almost five-fold higher than those with soluble MCP-1. These results indicate that the delivery of the mMCP-1 gene greatly enhanced antitumor effects following the apoptotic stimuli by promoting the recruitment and activation of macrophages and T lymphocytes, suggesting a novel strategy of immune-based gene therapy in the treatment of patients with HCC. © 2012 Macmillan Publishers Limited All rights reserved. 続きを見る

URL:

http://hdl.handle.net/2297/31396

タイトル・著者・出版者が同じ資料

詳細

主題:	hepatocellular carcinoma; herpes simplex virus thymidine kinase; membrane-bound form; monocyte chemoattractant protein-1; monocyte/macrophages
受理日:	2012-05-01

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1 論文 Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma Marukawa, Yohei, Nakamoto, Yasunari, Kakinoki, Kaheita, Tsuchiyama, Tomoya, Iida, Noriho, Kagaya, Takashi, Sakai, … Nature Publishing Group	7 論文 Tumor cell apoptosis induces tumor-specific immunity in a CC chemokine receptor 1- and 5-dependent manner in mice Iida, Noriho, Nakamoto, Yasunari, Baba, Tomohisa, Kakinoki, Kaheita, Li, Ying-Yi, Wu, Yu, Matsushita, Kouji, Kaneko, … Oxford University Press
2 論文 Optimal amount of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy against hepatocellular carcinoma by M1 macrophage … Tsuchiyama, Tomoya, Nakamoto, Yasunari, Sakai, Yoshio, Mukaida, Naofumi, Kaneko, Shuichi Japanese Cancer Association / Blackwell Publishing Ltd	8 論文 Frequency of CD45RO+ subset in CD4+CD25high regulatory T cells associated with progression of hepatocellular carcinoma Takata, Yoshiko, Nakamoto, Yasunari, Nakada, Akiko, Terashima, Takeshi, Arihara, Fumitaka, Kitahara, Masaaki, Kakinoki, … Elsevier
3 論文 Prevention of intrahepatic metastasis of liver cancer by suicide gene therapy and chemokine ligand 2/monocyte chemoattractant protein-1 delivery in mice Kakinoki, Kaheita, Nakamoto, Yasunari, Kagaya, Takashi, Tsuchiyama, Tomoya, Sakai, Yoshio, Nakahama, Tohru, Mukaida, … Wiley-Blackwell	9 論文 Identification of a secretory protein c19orf10 activated in hepatocellular carcinoma Sunagozaka, Hajime, Honda, Masao, Yamashita, Taro, Nishino, Ryuhei, Takatori, Hajime, Arai, Kuniaki, Yamashita, Tatsuya, … Wiley-Blackwell
4 論文 Prolonged, NK cell-mediated antitumor effects of suicide gene therapy combined with monocyte chemoattractant protein-1 against hepatocellular carcinoma. Tsuchiyama, T., Nakamoto, Yasunari, Sakai, Y., Mukaida, Naofumi, Sawabu, Norio, Kaneko, Shuichi 金沢大学がん研究所	10 論文 In vivo immunological antitumor effect of OK-432-stimulated dendritic cell transfer after radiofrequency ablation Nakagawa, Hidetoshi, Mizukoshi, Eishiro, Iida, Noriho, Terashima, Takeshi, Kitahara, Masaaki, Marukawa, Yohei, Kitamura, … Springer-Verlag
5 論文 Prolonged recurrence-free survival following OK432-stimulated dendritic cell transfer into hepatocellular carcinoma during transarterial embolization Nakamoto, Yasunari, Mizukoshi, Eishiro, Kitahara, Masaaki, Arihara, Fumitaka, Sakai, Yoshio, Kakinoki, Kaheita, Fujita, … Blackwell Publishing	11 その他 Enhanced antitumor effects of a bicistronic adenovirus vector expressing both herpes simplex virus thymidine kinase and monocyte chemoattractant protein-1 … Tsuchiyama, Tomoya, 土山, 智也金沢大学
6 論文 Antitumor effect after radiofrequency ablation of murine hepatoma is augmented by an active variant of CC chemokine ligand 3/macrophage inflammatory protein-1á Iida, Noriho, Nakamoto, Yasunari, Baba, Tomohisa, Nakagawa, Hidetoshi, Mizukoshi, Eishiro, Naito, Makoto, Mukaida, … American Association for Cancer Research	12 論文 Response to chemotherapy improves hepatic reserve for patients with hepatocellular carcinoma and Child–Pugh B cirrhosis Terashima, Takeshi, Yamashita, Tatsuya, Arai, Kuniaki, Kawaguchi, Kazunori, Kitamura, Kazuya, Yamashita, Taro, Sakai, … Japanese Cancer Association / Blackwell Publishing Ltd