Obesity-induced increase of CYP2E1 activity and its effect on disposition kinetics of chlorzoxazone in Zucker rats

フォーマット:

論文

責任表示:

Khemawoot, Phisit ; Yokogawa, Koichi ; Shimada, Tsutomu ; Miyamoto, Kenichi

言語:

英語

出版情報:

Elsevier, 2007-01-01

著者名:

• Khemawoot, Phisit
• Yokogawa, Koichi
• Shimada, Tsutomu
• Miyamoto, Kenichi

掲載情報:

Biochemical Pharmacology73

ISSN:

0006-2952      

巻:

73

通号:

1

開始ページ:

155

終了ページ:

162

概要:

金沢大学医学部附属病院薬剤部<br />This study was designed to investigate the induction of CYP2E1 in obese Zucker rats and its effect on the disposition kinetics of chlorzoxazone (CZX). CZX 20 mg/kg was administered to three groups of rats: normal Z … ucker rats fed a normal diet (ND), normal Zucker rats fed a high-fat diet (HF), and genetically obese Zucker rats fed a normal diet (OB). The values of the area under the plasma concentration–time curve from 0 to 1 (AUC1) of CZX were in the order of ND > HF > OB rats. The AUC1 values of total 6-hydroxychlorzoxazone (6OHCZX-T), which is considered to be a CYP2E1 metabolic marker, were in the opposite order. The values of the AUC1 ratio (6OHCZX–T/CZX) in ND, HF and OB rats were approximately 0.2, 0.3 and 0.4, respectively. The CZX concentration in fat was much higher than the concentrations in plasma, liver and kidney in all groups. Induction of CYP2E1 protein was greater in both liver and fat of OB rats than in those of HF rats. Microsomal activity of CYP2E1 in liver and fat was also in the order of OB > HF > NMrats. These results suggest that CYP2E1 may be induced in liver and fat of obese patients, thereby potentially altering the disposition kinetics of not only CZX, but also other lipophilic drugs metabolized by CYP2E1.©2006 Published by Elsevier Inc. 続きを見る

URL:

http://hdl.handle.net/2297/3444