Expression of bone morphogenetic protein 2 and fibroblast growth factor 2 during bone regeneration using different implant materials as an onlay bone graft in rabbit mandibles

フォーマット:

論文

責任表示:

Alam, Shamiul ; Ueki, Koichiro ; Marukawa, Kohei ; Ohara, Teruhisa ; Hase, Takashi ; Takazakura, Daisuke ; Nakagawa, Kiyomasa

言語:

英語

出版情報:

Elsevier, 2007-01-01

著者名:

Alam, Shamiul

Ueki, Koichiro

Marukawa, Kohei

Ohara, Teruhisa

Hase, Takashi

Takazakura, Daisuke

Nakagawa, Kiyomasa

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掲載情報:

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics

ISSN:

1079-2104      

巻:

103

通号:

1

開始ページ:

16

終了ページ:

26

バージョン:

author

概要:

金沢大学医学部附属病院歯科口腔外科<br />Objectives: The purpose of this study was to histologically and immunohistochemically evaluate bone regeneration using 3 different implant materials in rabbit mandibles and to compare the bone regenerative capab … ility of these materials in an animal model. Study design: Adult male Japanese white rabbits (n = 48; 12-16 wks old; 2.5-3.0 kg) were divided into 4 groups, consisting of 12 animals each. The implant materials were β-tricalcium phosphate (β-TCP), autologous bone derived from the radius, and recombinant human bone morphogenetic protein 2 (rhBMP-2) with polylactic acid/polyglycolic acid copolymer and gelatin sponge (PGS) complex. After incising along the inferior border of the mandible, the materials were implanted as only grafts and covered by titanium mesh with screws. No material was implanted into the control group. The rabbits were killed at 2, 4, 8, 12, and 24 wks postoperatively, and formalin-fixed specimens containing titanium mesh were embedded in acrylic resin. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with BMP-2 and fibroblast growth factor 2 (FGF-2) antibodies. Finally, they were examined microscopically. Results: The autologous bone induced substantially more new bone formation compared with β-TCP at 4 wks postoperatively. However, rhBMP-2/PGS induced new bone formation at 8 wks postoperatively. No growth of bony tissue was observed in the control group at any period. In the autologous bone and rhBMP-2/PGS groups, both BMP-2 and FGF-2 were observed later in the β-TCP group than in other groups. Conclusion: This study suggests that autologous bone as well as rhBMP-2/PGS implants induce expression of both BMP-2 and FGF-2 specifically at the operated sites, even at early stages. © 2007 Mosby, Inc. All rights reserved. 続きを見る

URL:

http://hdl.handle.net/2297/3470