In vivo immunological antitumor effect of OK-432-stimulated dendritic cell transfer after radiofrequency ablation

フォーマット:

論文

責任表示:

Nakagawa, Hidetoshi ; Mizukoshi, Eishiro ; Iida, Noriho ; Terashima, Takeshi ; Kitahara, Masaaki ; Marukawa, Yohei ; Kitamura, Kazuya ; Nakamoto, Yasunari ; Hiroishi, Kazumasa ; Imawari, Michio ; Kaneko, Shuichi

言語:

英語

出版情報:

Springer-Verlag, 2014-04-01

著者名:

Nakagawa, Hidetoshi

Mizukoshi, Eishiro

Iida, Noriho

Terashima, Takeshi

Kitahara, Masaaki

Marukawa, Yohei

Kitamura, Kazuya

Nakamoto, Yasunari

Hiroishi, Kazumasa

Imawari, Michio

Kaneko, Shuichi

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掲載情報:

Cancer Immunology, Immunotherapy

ISSN:

0340-7004      

巻:

63

通号:

4

開始ページ:

347

終了ページ:

356

バージョン:

author

概要:

Radiofrequency ablation therapy (RFA) is a radical treatment for liver cancers and induces tumor antigen-specific immune responses. In the present study, we examined the antitumor effects of focal OK-432-stimulated dendritic cell (DC) transfer combined with RFA and analyzed the functional mechanisms involved using a murine model. C57BL/6 mice were injected subcutaneously with colon cancer cells (MC38) in their bilateral flanks. After the establishment of tumors, the … subcutaneous tumor on one flank was treated using RFA, and then OK-432-stimulated DCs were injected locally. The antitumor effect of the treatment was evaluated by measuring the size of the tumor on the opposite flank, and the immunological responses were assessed using tumor-infiltrating lymphocytes, splenocytes and draining lymph nodes. Tumor growth was strongly inhibited in mice that exhibited efficient DC migration after RFA and OK-432-stimulated DC transfer, as compared to mice treated with RFA alone or treatment involving immature DC transfer. We also demonstrated that the antitumor effect of this treatment depended on both CD8-positive and CD4-positive cells. On the basis of our findings, we believe that combination therapy for metastatic liver cancer consisting of OK-432-stimulated DCs in combination with RFA can proceed to clinical trials, and it is anticipated to be markedly superior to RFA single therapy. © 2013 Springer-Verlag Berlin Heidelberg. 続きを見る

URL:

http://hdl.handle.net/2297/36504