カラムスイッチングHPLCシステムによるゾニサミドの高感度測定法の評価

フォーマット:

論文

責任表示:

金, 明姫 ; 西尾, 忠 ; 嶋田, 努 ; 竹田, 和喜 ; 横川, 弘一 ; 宮本, 謙一

言語:

日本語

出版情報:

日本医療薬学会 = Japanese Society of Pharmaceutical Health Care and Sciences, 2003-01-01

著者名:

金, 明姫

西尾, 忠

嶋田, 努

竹田, 和喜

横川, 弘一

宮本, 謙一

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掲載情報:

医療薬学 = Japanese journal of pharmaceutical health care and sciences

ISSN:

1346-342x      

巻:

29

通号:

2

開始ページ:

178

終了ページ:

183

バージョン:

publisher

概要:

A fully automated high-performance liquid chromatography system with column-switching was evaluated to determine the concentration of zonisamide (ZNS) in human plasma. After the plasma sample was diluted to 10-times with the mobile phase, the sample (101μL) was injected into the column-switching system without pretreatment. ZNS was detected by ultraviolet absorption at 246 nm. The calibration curve of ZNS was linear and ranged from 1.57 to 50μg/mL … of plasma. The coefficients of variation in the within-run (n=5) and the between-run (5 days) precisions of ZNS were below 5 %. The limit of quantification was 0.5pg/mL in 1 μL of human plasma. A good correlation was found between the data obtained by this system and the ELISA system using Markit-M Excegran (r=0.956) or the solid-phase-extraction HPLC method (r=0.985). This system for ZNS is thus considered to be a very useful measurement for therapeutic drug monitoring because of its high sensitivity, rapidity and low cost compared with other measurement systems. 続きを見る

URL:

http://hdl.handle.net/2297/39138