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JSAP1 and JLP are required for ARF6 localization to the midbody in cytokinesis
- フォーマット:
- 論文
- 責任表示:
- Tuvshintugs, Baljinnyam ; Sato, Tokiharu ; Enkhtuya, Radnaa ; Yamashita, Katsumi ; Yoshioka, Katsuji
- 言語:
- 英語
- 出版情報:
- Blackwell Publishing, 2014-09-01
- 著者名:
- 掲載情報:
- Genes to Cells
- ISSN:
- 1356-9597
- 巻:
- 19
- 通号:
- 9
- 開始ページ:
- 692
- 終了ページ:
- 703
- バージョン:
- author
- 概要:
- The ADP-ribosylation factor 6 (ARF6) GTPase is important in cytokinesis and localizes to the midbody. However, the mechanism and regulation of ARF6's recruitment to the midbody are largely unknown. Here, we investigated the functions of two binding partners of active ARF6, c-Jun NH2-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1) and JNK-associated leucine zipper protein (JLP), by gene knockout and rescue … experiments in mouse embryonic fibroblasts. Depleting both JSAP1 and JLP impaired ARF6's localization to the midbody and delayed cytokinesis. These defects were almost completely rescued by wild-type JSAP1 or JLP, but not by JSAP1 or JLP mutants that were unable to interact with active ARF6 or with the kinesin heavy chain (KHC) of kinesin-1. In transfected cells, a constitutively active form of ARF6 associated with KHC only when co-expressed with wild-type JSAP1 or JLP and not with a JSAP1 or JLP mutant. These findings suggest that JSAP1 and JLP, which might be paralogous to each other, are critical and functionally redundant in cytokinesis and control ARF6 localization to the midbody by forming a tripartite complex of JSAP1/JLP, active ARF6, and kinesin-1. © 2014 The Authors. Genes to Cells © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd. 続きを見る
- URL:
- http://hdl.handle.net/2297/40115
類似資料:
金沢大学がん研究所 = Kanazawa University Cancer Research Institute | |
金沢大学がん研究所 = Kanazawa University Cancer Research Institute | |
Institute of Science and Engineering, Kanazawa University = 金沢大学 |
金沢大学がん研究所 = Kanazawa University Cancer Research Institute |
Springer Science+Business Media B.V. |
日本生化学会 = Japanese Biochemical Society |