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A novel resting form of the trinuclear copper center in the double mutant of a multicopper oxidase, CueO, Cys500Ser/Glu506Ala

フォーマット:
論文
責任表示:
Kajikawa, Takao ; Sugiyama, Ryosuke ; Kataoka, Kunishige ; Sakurai, Takeshi
言語:
英語
出版情報:
Elsevier, 2015-08-01
著者名:
掲載情報:
Journal of Inorganic Biochemistry
ISSN:
0162-0134  CiNii Research  Webcat Plus  JAIRO
巻:
149
開始ページ:
88
終了ページ:
90
バージョン:
author
概要:
A multicopper oxidase, CueO was doubly mutated at its type I copper ligand, Cys500 and an acidic amino acid residue located in the proton transfer pathway, Glu506, to Ser and Ala, respectively. Cys500Ser/Glu506Ala was mainly in a novel resting form to afford the absorption band at ca. 400. nm and an EPR signal with a highly anisotropic character derived from type III copper. However, Cys500Ser/Glu506Ala gave the same reaction intermediate (peroxide intermediate) as that from Cys500Ser and Cys500Ser/Glu506Gln. © 2015 Elsevier Inc.<br />Embargo Period 24 months 続きを見る
URL:
http://hdl.handle.net/2297/42216
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