Cell fate of müller cells during photoreceptor regeneration in an N-methyl-N-nitrosourea- induced retinal degeneration model of zebrafish

フォーマット:

論文

責任表示:

Ogai, Kazuhiro ; Hisano, Suguru ; Sugitani, Kayo ; Koriyama, Yoshiki ; Kato, Satoru

言語:

英語

出版情報:

Springer, 2016-10-01

著者名:

• Ogai, Kazuhiro
• Hisano, Suguru
• Sugitani, Kayo
• Koriyama, Yoshiki
• Kato, Satoru

掲載情報:

Advances in Experimental Medicine and Biology

ISSN:

0065-2598      

巻:

854

開始ページ:

685

終了ページ:

692

バージョン:

author

概要:

Zebrafish can regenerate several organs such as the tail fin, heart, central nervous system, and photoreceptors. Very recently, a study has demonstrated the photoreceptor regeneration in the alkylating agent N-methyl-N-nitrosourea (MNU)- induced retinal degeneration (RD) zebrafish model, in which whole photoreceptors are lost within a week after MNU treatment and then regenerated within a month. The research has also shown massive proliferation of Müller cells within a … week. To address the question of whether proliferating Müller cells are the source of regenerating photoreceptors, which remains unknown in the MNU-induced zebrafish RD model, we employed a BrdU pulse-chase technique to label the proliferating cells within a week after MNU treatment. As a result of the BrdU pulse-chase technique, a number of BrdU+ cells were observed in the outer nuclear layer as well as the inner nuclear layer. This implies that regenerating photoreceptors are derived from proliferating Müller cells in the zebrafish MNU-induced RD model. © Springer International Publishing Switzerland 2016.<br />[Book Chapter] 続きを見る

URL:

http://hdl.handle.net/2297/43910