Immunohistochemical analysis of CYP2A13 in various types of human lung cancers

フォーマット:

論文

責任表示:

Fukami, Tatsuki ; Nakajima, Miki ; Matsumoto, Isao ; Zen, Yoh ; Oda, Makoto ; Yokoi, Tsuyoshi

言語:

英語

出版情報:

Japanese Cancer Association / Blackwell Publishing Ltd, 2010-04-01

著者名:

Fukami, Tatsuki

Nakajima, Miki

Matsumoto, Isao

Zen, Yoh

Oda, Makoto

Yokoi, Tsuyoshi

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掲載情報:

Cancer Science

ISSN:

1347-9032      

巻:

101

通号:

4

開始ページ:

1024

終了ページ:

1028

バージョン:

publisher

概要:

Human CYP2A13, which is expressed in the respiratory tract, is the most efficient enzyme for the metabolic activation of tobacco-specific nitrosamines such as 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). The relevance of CYP2A13 in carcinogenicity and toxicity in the respiratory tract has been suggested, but the expression of CYP2A13 protein in lung cancer tissues remains to be determined. We first prepared a mouse monoclonal antibody against human … CYP2A13. The antibody showed no cross reactivity with the other CYP isoforms including CYP2A6. Using the specific antibody, we performed immunohistochemical analysis for human lung carcinomas. In adenocarcinomas (n=15), all specimens were positive for the staining and five samples showed strong staining. In squamous cell carcinomas (n=15) and large cell carcinomas (n=15), each 14 samples were positive for the staining and two and three samples showed strong staining, respectively. In small cell carcinoma samples (n=15), eight samples were negative for the staining and five samples showed weak or moderate staining. In conclusion, we first found that the expression of CYP2A13 was markedly increased in non-small cell lung carcinomas. The high expression might be associated with the tumor development and progression in non-small cell lung carcinomas. © 2010 Japanese Cancer Association. 続きを見る

URL:

http://hdl.handle.net/2297/45570