Utilization of Liver Microsomes to Estimate Hepatic Intrinsic Clearance of Monoamine Oxidase Substrate Drugs in Humans

フォーマット:

論文

責任表示:

Masuo, Yusuke ; Nagamori, Shushi ; Hasegawa, Aoi ; Hayashi, Kazuki ; Isozumi, Noriyoshi ; Nakamichi, Noritaka ; Kanai, Yoshikatsu ; Kato, Yukio

言語:

英語

出版情報:

Springer, 2017-06-01

著者名:

Masuo, Yusuke

Nagamori, Shushi

Hasegawa, Aoi

Hayashi, Kazuki

Isozumi, Noriyoshi

Nakamichi, Noritaka

Kanai, Yoshikatsu

Kato, Yukio

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掲載情報:

Pharmaceutical Research

ISSN:

0724-8741      

巻:

34

通号:

6

開始ページ:

1233

終了ページ:

1243

バージョン:

author

概要:

Purpose: Monoamine oxidases (MAOs) are non-CYP enzymes that contribute to systemic elimination of therapeutic agents, and localized on mitochondrial membranes. The aim of the present study was to validate quantitative estimation of metabolic clearance of MAO substrate drugs using human liver microsomes (HLMs). Methods: Three MAO substrate drugs, sumatriptan, rizatriptan and phenylephrine, as well as four CYP substrates were selected, and their … disappearance during incubation with HLMs or mitochondria (HLMt) was measured. Metabolic clearance (CL) was then calculated from the disappearance curve. Results: CL obtained in HLMs for sumatriptan and a typical MAO substrate serotonin was correlated with that obtained in HLMt among ten human individual livers. Hepatic intrinsic clearance (CLint,vitro) estimated from CL in HLMs was 14–20 and 2–5 times lower than in vivo hepatic intrinsic clearance (CLint,vivo) obtained from literature for MAO and CYP substrates, respectively. Utilization of HLMs for quantitatively assessing metabolic clearance of MAO substrates was further validated by proteomics approach which has revealed that numerous proteins localized on inner and outer membranes of mitochondria were detected in both HLMs and HLMt. Conclusion: CLint,vitro values of MAO substrate drugs can be quantitatively estimated with HLMs and could be used for semi-quantitative prediction of CLint,vivo values. © 2017 Springer Science+Business Media New York<br />Embargo Period 12 months 続きを見る

URL:

http://hdl.handle.net/2297/47882