99mTc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time

フォーマット:

論文

責任表示:

Taki, Junichi ; Higuchi, Takahiro ; Kawashima, Atsuhiro ; Tait, Jonathan F. ; Muramori, Akira ; Matsunari, Ichiro ; Nakajima, Kenichi ; Vanderheyden, Jean-Luc ; Strauss, H. William

言語:

英語

出版情報:

日本循環器学会, 2007-01-01

著者名:

Taki, Junichi

Higuchi, Takahiro

Kawashima, Atsuhiro

Tait, Jonathan F.

Muramori, Akira

Matsunari, Ichiro

Nakajima, Kenichi

Vanderheyden, Jean-Luc

Strauss, H. William

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掲載情報:

Circulation Journal

ISSN:

1346-9843      

巻:

71

通号:

7

開始ページ:

1141

終了ページ:

1146

バージョン:

publisher

概要:

金沢大学医学部附属病院核医学診療科<br />Background: To determine whether mild to moderate ischemia that is not severe enough to induce myocardial infarction will cause myocardial cell damage or apoptosis, the 99mTc-Annexin-V (Tc-A) uptake was studied … in groups of rats with various intervals of coronary occlusion and reperfusion times. Methods and Results: After left coronary artery occlusion for 15 min (n=23), 10 min (n=23), or 5min (n=12), Tc-A (80-150 MBq) was injected at 0.5, 1.5, 6, or 24 h after reperfusion. One hour later, to verify the area at risk, 201Tl (0.74 MBq) was injected just after left coronary artery re-occlusion and the rats were killed 1 min later. Dual tracer autoradiography was performed to assess Tc-A uptake and area at risk. In all 5-min occlusion and reperfusion models, no significant Tc-A uptake was observed in the area at risk. Tc-A uptake ratios in the 15-min and 10-min ischemia models were 4.46±3.16 and 2.02±0.47 (p=0.078) at 0.5 h after reperfusion, 3.49±1.78 and 1.47±0.11 (p<0.05) at 1.5h after reperfusion, 1.60±0.43 and 1.34±0.23 (p=0.24) at 6h after reperfusion, 1.50±0.33 and 1.28±0.33 (p=0.099) at 24 h after reperfusion, respectively. With 15-min ischemia, in 3 of the 5 rats there were a few micro-foci of myocardial cell degeneration and cell infiltration in less than 1% of the ischemic area at 24 h after reperfusion. No significant histological change was observed in rats with 10-min or 5-min ischemia. Conclusion: The data indicate that Tc-A binding depends on the severity of ischemia even without a significant amount of histological change or infarction. 続きを見る

URL:

http://hdl.handle.net/2297/6863