※一部利用できない機能があります
| 1.
論文 |
論文
|
Hoshikawa, Takaya ; Tanji, Kei ; Matsuo, Jun-ichi ; Ishibashi, Hiroyuki
概要:
Intramolecular [2+2] cycloaddition of γ,δ-unsaturated ketenes derived from hex-5-enoyl chloride derivatives gave bicyclo[2.1.1]hexan-5-ones with complete regioselectivity. © 2012 The Pharmaceutical Society of Japan.
|
||||||||||||||||||
| 2.
論文 |
論文
|
Nakano, Takeo ; Soeta, Takahiro ; Endo, Kohei ; Inomata, Katsuhiko ; Ukaji, Yutaka
概要:
The sequential 1,4-elimination reaction of (E)-4-alkoxy-2-butenyl benzoates and [1,2]-Wittig rearrangement gave (2Z,4E)-
…
2,4-pentadien-1-ols stereoselectively. Z-Selective formation of intermediary vinyl ethers, whose stereochemistry was well elucidated by the "syn-effect", was achieved by treatment of the 2-butenyl benzoates with KOH in the presence of Pd catalyst. The subsequent [1,2]-Wittg rearrangement by use of n-BuLi proceeded with retention of the stereochemistry of the intermediary vinyl ethers. © 2013 American Chemical Society.
続きを見る
|
||||||||||||||||||
| 3.
論文 |
論文
|
Higuchi, Akihiro ; Erina Oonishi ; Kawakita, Tetsuya ; Tsubota, Kazuo ; 樋口, 明弘
概要:
金沢大学先端科学・社会共創推進機構<br />Purpose: 2-hydroxy estradiol (2-OHE2) is a catechol derivative of 17β -Estradiol (E2) and it is s
…
ynthesized from E2 catalyzed by cytochrome P4501A1. Previous studies reported that 2-OHE2 is a physiologic antioxidant in lipoproteins, liver microsomes, and the brain. Catechol derivatives show an anti-inflammatory effect through the inhibition of prostaglandin endoperoxide synthase (PGS) activity. Corneal erosion caused by dry eye is related to an increase in oxidative stress and inflammation in ocular surface cells. We investigated the therapeutic effects of 2-OHE2 on corneal damage caused by dry eye. Methods: Steroidal radical scavenging activity was confirmed through the electron spin resonance (ESR) method. PGS activity was measured using the COX Fluorescent Activity Assay Kit. To evaluate the effect of 2-OHE2 on the treatment for dry eye, 2-OHE2 was applied as an eye drop experiment using dry eye model rats. Results: 2-OHE2 scavenged tyrosyl radical and possibly suppressed oxidative stress in corneal epithelial cells. In addition, 2-OHE2 inhibited PGS activity, and 2-OHE2 is probably a competitive inhibitor of PGS. Corneal PGS activity was upregulated in the dry eye group. Therefore, 2-OHE2 eye drops improved corneal erosion in dry eye model rats. Conclusions: 2-OHE2 is a candidate for the treatment of dry eye through the suppression of inflammation and oxidative stress in the cornea. © 2016 Molecular Vision.
続きを見る
|
||||||||||||||||||
| 4.
論文 |
論文
|
Nakata, Shunji ; Kato, Takashi ; Ozaki, Shinya ; Kawae, Takeshi ; Morimoto, Akiharu
概要:
Thin film Al2O3/Al-rich Al2O 3/SiO2 structures were fabricated on p-Si substrates. Radio-frequency magnetron co-sputteri
…
ng was used to form Al-rich Al 2O3 thin film as the charge-trapping layer of nonvolatile Al2O3 memory. Capacitance-voltage measurements showed a large hysteresis due to charge trapping in the Al-rich Al2O 3 layer. The charge trap density was estimated to be 42.7 × 1018 cm- 3, which is the largest value ever reported for an Al-rich Al2O3 layer; it is six times larger than that of a conventional metal-nitride-oxide-silicon memory. Thermal annealing was found to reduce the leakage current of the Al2O3 blocking layer, thereby providing this structure with better data retention at room temperature than an as-deposited one. In addition, the annealed structure was found to exhibit good data retention even at 100 C. © 2013 Elsevier B.V. All rights reserved.
続きを見る
|
||||||||||||||||||
| 5.
論文 |
論文
|
Matsuo, Jun-ichi ; Okuno, Ryosuke ; Takeuchi, Kosuke ; Kawano, Mizuki ; Ishibashi, Hiroyuki
概要:
金沢大学医薬保健研究域薬学系<br />Optimized reaction conditions for the preparation of various 2-monosubstituted 3-ethoxycyclobutanone
…
s are described. 2-Monoalkyl 3-ethoxycyclobutanones were efficiently prepared by the reaction of the corresponding carboxylic acid chlorides and an excess amount of ethyl vinyl ether in the presence of diisopropylethylamine at 90 °C in a sealed tube. 2-Monoaryl 3-ethoxycyclobutanones were prepared by using 2,6-lutidine as a base in the above-mentioned procedure. © 2010 Published by Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 6.
論文 |
論文
|
Matsuo, Junichi ; Okuno, Ryosuke ; Takeuchi, Kosuke ; Kawano, Mizuki ; Ishibashi, Hiroyuki
概要:
金沢大学医薬保健研究域薬学系<br />Optimized reaction conditions for the preparation of various 2-monosubstituted 3-ethoxycyclobutanone
…
s are described. 2-Monoalkyl 3-ethoxycyclobutanones were efficiently prepared by the reaction of the corresponding carboxylic acid chlorides and an excess amount of ethyl vinyl ether in the presence of diisopropylethylamine at 90 °C in a sealed tube. 2-Monoaryl 3-ethoxycyclobutanones were prepared by using 2,6-lutidine as a base in the above-mentioned procedure. © 2010 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 7.
論文 |
論文
|
Okado, Ryohei ; Nowaki, Aya ; Matsuo, Jun-ichi ; Ishibashi, Hiroyuki
概要:
A catalytic amount of tin(IV) chloride catalyzed formal [4+2] cycloaddition reaction of di-tert-butyl 2-ethoxycyclobutane-1,1-carboxylate with ketones or aldehydes to give diethyl 6-ethoxydihydro-2H-pyran-3,3(4H)-dicarboxylates,
…
whereas two equivalents of trimethylsilyl triflate promoted tandem [4+2] cycloaddition and lactonization to afford 3-oxo-2,6-dioxabicyclo[2.2.2]octane-4-carboxylate esters.
続きを見る
|
||||||||||||||||||
| 8.
論文 |
論文
|
Yokota, Shinichi ; Higashi, Eriko ; Fukami, Tatsuki ; Yokoi, Tsuyoshi ; Nakajima, Miki
概要:
金沢大学医薬保健研究域薬学系<br />Human CYP2A6 is responsible for the metabolism of nicotine and coumarin as well as the metabolic act
…
ivation of tobacco-related nitrosamines. Earlier studies revealed that CYP2A6 activity was increased by dietary cadmium or cruciferous vegetables, but the underlying mechanisms remain to be clarified. In the present study, we investigated the possibility that Nrf2 might be involved in the regulation of CYP2A6. Real-time RT-PCR analysis revealed that the CYP2A6 mRNA level in human hepatocytes was significantly (P < 0.01, 1.4-fold) induced by 10 μM sulforaphane (SFN), a typical activator of Nrf2. A computer-based search identified three putative antioxidant response elements (AREs) in the 5′-flanking region of the CYP2A6 gene at positions -1212, -2444, and -3441, termed ARE1, ARE2, and ARE3, respectively. Electrophoretic mobility shift assays demonstrated that Nrf2 bound only to ARE1. Luciferase assays using HepG2 cells revealed that the overexpression of Nrf2 significantly increased the reporter activities of the constructs containing a 30-bp fragment that included ARE1. However, the activity of the construct containing the intact 5′-flanking region (-1 to -1395) including ARE1 was not increased by the overexpression of Nrf2. In contrast, when the reporter construct was injected into mice via the tail vein, the reporter activity in the liver was significantly (P < 0.05, 1.9-fold) increased by SFN (1 mg/head) administration. In conclusion, we found that human CYP2A6 is regulated via Nrf2, suggesting that CYP2A6 is induced under oxidative stress. © 2010 Elsevier Inc. All rights reserved.
続きを見る
|
||||||||||||||||||
| 9.
電子ブック |
ＥＢ
|
|
||||||||||||||||||
| 10.
論文 |
論文
|
Miyamoto, Yoshiaki ; Wada, Norihiro ; Soeta, Takahiro ; Fujinami, Shuhei ; Inomata, Katsuhiko ; Ukaji, Yutaka
概要:
The stereoselective direct transformation of N-(propargylic)hydroxylamines into cis-2-acylaziridines was achieved by the
…
combined use of AgBF4 and CuCl. Copper salts were found to promote the transformation of the intermediary 4-isoxazolines into 2-acylaziridines and both 3-aryl- and 3-alkyl-substituted 2-acylaziridines could be prepared by using this method. Furthermore, subsequent 1,3-dipolar cycloaddition of azomethine ylides that were generated in situ from the intermediary 2-acylaziridines with maleimides was achieved in a stereoselective one-pot procedure to afford the corresponding 2-acylpyrrolidines, which consisted of an octahydropyrrolo[3,4-c]pyrrole skeleton. Love the way ylide: The transformation of N-(propargylic) hydroxylamines into cis-2-acylaziridines and subsequent 1,3-dipolar cycloaddition of the in situ generated azomethine ylides with maleimides stereoselectively afforded 2-acylpyrrolidines. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
続きを見る
|
||||||||||||||||||
| 11.
論文 |
論文
|
Popivanova, Boryana K. ; Kostadinova, Feodora I. ; Mukaida, Naofumi
概要:
Azoxymethane (AOM) administration followed by repetitive dextran sulfate sodium (DSS) ingestion causes chronic colonic inflammation with macrophage infiltration and enhanced expression of a macrophage-tropic chemokine, CCL2, in wild-type
…
(WT) mice. These mice eventually develop multiple colon tumors. In contrast, mice deficient in CCR2, a specific receptor for CCL2, exhibited less macrophage infiltration and attenuated tumor formation. WT mice transplanted with CCR2-deficient bone marrow developed fewer tumors after AOM and DSS treatment than either WT or CCR2-deficient mice transplanted with WT bone marrow. Furthermore, when injected to WT mice with multiple colon tumors, a CCL2 antagonist expression vector attenuated macrophage and granulocyte infiltration, and eventually reduced the numbers and sizes of tumors. These results implied the crucial involvement of the CCL2-CCR2 interactions in the development and progression of colon carcinoma associated with chronic inflammation.
続きを見る
|
||||||||||||||||||
| 12.
論文 |
論文
|
Begum, Zinnat A. ; Rahman, Ismail M. M. ; Tate, Yousuke ; Egawa, Yuji ; Maki, Teruya ; Hasegawa, Hiroshi
概要:
The protonation and complex formation equilibria of two biodegradable aminopolycarboxylate chelants {dl-2-(2-carboxymeth
…
yl)nitrilotriacetic acid (GLDA) and 3-hydroxy-2,2′-iminodisuccinic acid (HIDS)} with Ni 2+, Cu 2+, Zn 2+, Cd 2+ and Pb 2+ ions were investigated using the potentiometric method at a constant ionic strength of I = 0.10 mol·dm -3 (KCl) in aqueous solutions at 25 ± 0.1 °C. The stability constants of the proton-chelant and metal-chelant species for each metal ion were determined, and the concentration distributions of various complex species in solution were evaluated for each ion. The stability constants (log 10K ML) of the complexes containing Ni 2+, Cu 2+, Zn 2+, Cd 2+ and Pb 2+ ions followed the identical order of log 10K CuL > log 10K NiL > log 10K PbL > log 10K ZnL > log 10K CdL for either GLDA (13.03 > 12.74 > 11.60 > 11.52 > 10.31) or HIDS (12.63 > 11.30 > 10.21 > 9.76 > 7.58). In each case, the constants obtained for metal-GLDA complexes were larger than the corresponding constants for metal-HIDS complexes. The conditional stability constants (log 10 {Mathematical expression}) of the metal-chelant complexes containing GLDA and HIDS were calculated in terms of pH, and compared with the stability constants for EDTA and other biodegradable chelants. © 2012 Springer Science+Business Media New York.
続きを見る
|
||||||||||||||||||
| 13.
論文 |
論文
|
Begum, Zinnat A. ; Rahman, Ismail M. M. ; Tate, Yousuke ; Egawa, Yuji ; Maki, Teruya ; Hasegawa, Hiroshi
概要:
The protonation and complex formation equilibria of two biodegradable aminopolycarboxylate chelants {dl-2-(2-carboxymeth
…
yl)nitrilotriacetic acid (GLDA) and 3-hydroxy-2,2′-iminodisuccinic acid (HIDS)} with Ni 2+, Cu2+, Zn2+, Cd2+ and Pb 2+ ions were investigated using the potentiometric method at a constant ionic strength of I = 0.10 mol·dm-3 (KCl) in aqueous solutions at 25 ± 0.1 C. The stability constants of the proton-chelant and metal-chelant species for each metal ion were determined, and the concentration distributions of various complex species in solution were evaluated for each ion. The stability constants (log10 K ML) of the complexes containing Ni2+, Cu2+, Zn2+, Cd2+ and Pb2+ ions followed the identical order of log10 K CuL > log10 K NiL > log10 K PbL > log10 K ZnL > log10 K CdL for either GLDA (13.03 > 12.74 > 11.60 > 11.52 > 10.31) or HIDS (12.63 > 11.30 > 10.21 > 9.76 > 7.58). In each case, the constants obtained for metal-GLDA complexes were larger than the corresponding constants for metal-HIDS complexes. The conditional stability constants (log10 $$ K-{\text{ML}}^{'} $$) of the metal-chelant complexes containing GLDA and HIDS were calculated in terms of pH, and compared with the stability constants for EDTA and other biodegradable chelants. © 2012 Springer Science+Business Media New York.
続きを見る
|
||||||||||||||||||
| 14.
図書 |
図書
|
Nagarjuna ; aus dem chinesischen Text des Kumarajiva übersetzt und mit einem Kommentar herausgegeben von Lutz Geldsetzer
|
||||||||||||||||||
| 15.
論文 |
論文
|
Kato, Takehiro ; Kotaka, Hiroki ; Ishii, Fumiyuki
概要:
Using first-principles method, we investigated the electronic states of Bi2Te3 and Bi2Se3. We showed that both Bi2Te3 an
…
d Bi2Se3 are insulators with a bulk band gap. In contrast, the surface states of Bi2Te3 and Bi2Se3 films have a metallic band connecting the conduction and valence bands. The films have an energy gap at the (Formula presented.) point when the film thickness is less than four quintuple layers (QLs), or about 30 Å. The energy gaps are closed at six QLs and four QLs for Bi2Te3 and Bi2Se3, respectively. We confirmed the metallicity up to nine QLs. Furthermore, we investigated the spin structures of nine-QL films at the Fermi energy in momentum space. We found that both the Bi2Te3 and Bi2Se3 films have Rashba-type spin textures; i.e. the surface states have spin–polarisation. To investigate the spatial distribution of the spin, we decomposed the expected values of the spin for each atom. The expected values of the spin are localised within the third QL from the surface. Our results of nine-QL films clearly show the boundary between the bulk and surface regions.
続きを見る
|
||||||||||||||||||
| 16.
論文 |
論文
|
Echizen, Kanae ; Hirose, Osamu ; Maeda, Yusuke ; Oshima, Masanobu
概要:
Cyclooxygenase-2 (COX-2) and its downstream product prostaglandin E2 (PGE2) play a key role in generation of the inflamm
…
atory microenvironment in tumor tissues. Gastric cancer is closely associated with Helicobacter pylori infection, which stimulates innate immune responses through Toll-like receptors (TLRs), inducing COX-2/PGE2 pathway through nuclear factor-κB activation. A pathway analysis of human gastric cancer shows that both the COX-2 pathway and Wnt/β-catenin signaling are significantly activated in tubular-type gastric cancer, and basal levels of these pathways are also increased in other types of gastric cancer. Expression of interleukin-11, chemokine (C-X-C motif) ligand 1 (CXCL1), CXCL2, and CXCL5, which play tumor-promoting roles through a variety of mechanisms, is induced in a COX-2/PGE2 pathway-dependent manner in both human and mouse gastric tumors. Moreover, the COX-2/PGE2 pathway plays an important role in the maintenance of stemness with expression of stem cell markers, including CD44, Prom1, and Sox9, which are induced in both gastritis and gastric tumors through a COX-2/PGE2-dependent mechanism. In contrast, disruption of Myd88 results in suppression of the inflammatory microenvironment in gastric tumors even when the COX-2/PGE2 pathway is activated, indicating that the interplay of the COX-2/PGE2 and TLR/MyD88 pathways is needed for inflammatory response in tumor tissues. Furthermore, TLR2/MyD88 signaling plays a role in maintenance of stemness in normal stem cells as well as gastric tumor cells. Accordingly, these results suggest that targeting the COX-2/PGE2 pathway together with TLR/MyD88 signaling, which would suppress the inflammatory microenvironment and maintenance of stemness, could be an effective preventive or therapeutic strategy for gastric cancer. © 2016 Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 17.
論文 |
論文
|
Yamakawa, Daishi ; Kidoya, Hiroyasu ; Sakimoto, Susumu ; Jia, Weizhen ; Naito, Hisamichi ; Takakura, Nobuyuki ; 内藤, 尚道 ; 高倉, 伸幸
概要:
金沢大学医薬保健研究域医学系<br />Tie2 is a receptor tyrosine kinase expressed on vascular endothelial cells (ECs). It has dual roles
…
in promoting angiogenesis and stabilizing blood vessels, and it has been suggested that Tie2 forms dimers and/or oligomers in the absence of angiopoietin-1 (Ang1); however, the mechanism of ligand-independent dimerization of Tie2 and its biological significance have not been clarified. Using a bimolecular fluorescence complementation assay and a kinase-inactive Tie2 mutant, we show here that ligand-independent Tie2 dimerization is induced without Tie2 phosphorylation. Moreover, based on the fact that Tie1 never forms heterodimers with Tie2 in the absence of Ang1 despite having high amino acid sequence homology with Tie2, we searched for ligand-independent dimerization domains of Tie2 by reference to the difference with Tie1. We found that the YIA sequence of the intracellular domain of Tie2 corresponding to the LAS sequence in Tie1 is essential for this dimerization. When the YIA sequence was replaced by LAS in Tie2 (Tie2YIA/LAS), ligand-independent dimer was not formed in the absence of Ang1. When activation of Tie2YIA/LAS was induced by a high dose of Ang1, phosphorylation of Tie2 was limited compared with wild-type Tie2, resulting in retardation of activation of Erk downstream of Tie2. Therefore, these data suggest that ligand-independent dimerization of Tie2 is essential for a strong response upon stimulation with high dose Ang1. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
続きを見る
|
||||||||||||||||||
| 18.
論文 |
論文
|
18. The Effect of Tree Planting within Roadside Green Space on Dispersion of CO2 from Transportation
Aini, Nurul ; Shen, Zhenjiang
概要:
Transportation has become one of the most significant contributors to CO2 in the world because of its fuel usage. Trees are planted on the roadside to reduce levels of CO2 in the air because trees have the ability to absorb CO2 to be used
…
in the photosynthesis process. This ability will be maximized if the dispersion of CO2 is concentrated around the tree. However, there are some differing results from previous studies regarding this. Some research results have found trees can increase CO2 concentration and vice versa. Accordingly, this study aims to evaluate the effect of tree planting on the roadside in dispersing CO2 using a real 3D environment. The methods used in this research are CO2 emission analysis to obtain the amount of CO2. Then, Computational Fluid Dynamics (CFD) analysis is used to simulate the dispersion of CO2 in the study area both without trees and with trees. However, due to the mixing of air and CO2, this simulation uses a scalar mixing analysis. Some conditions are considered, such as the characteristics of buildings, the characteristics of trees, and environmental conditions. The result indicates that trees can decrease the velocity and increase the concentration of CO2 on the roadside but decrease CO2 concentration on the road. Tree planting can decrease the velocity by 4.3% in the value range 0.9-1 m/s. This condition increases CO2 concentration on the roadside. Trees can increase CO2 by 25% on the right of a roadside and increase CO2 concentration by 10% on the left side.
続きを見る
|
||||||||||||||||||
| 19.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 20.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 21.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 22.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 23.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 24.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 25.
論文 |
論文
|
Matsuo, Jun-ichi ; Okuno, Ryosuke ; Ishibashi, Hiroyuki
概要:
2-Carbomethoxycyclobutanone reacted with N-phenyl-C-arylnitrones to afford methyl 5-oxo-2-[aryl(phenylamino)methyl]tetra
…
hydrofuran-2-carboxylates by the catalysis of indium(III) triflate in the presence of magnesium sulfate. © 2012 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 26.
論文 |
論文
|
Matsuo, Jun-ichi ; Okuno, Ryosuke ; Ishibashi, Hiroyuki
概要:
2-Carbomethoxycyclobutanone reacted with N-phenyl-C-arylnitrones to afford methyl 5-oxo-2-[aryl(phenylamino)methyl]tetra
…
hydrofuran-2-carboxylates by the catalysis of indium(III) triflate in the presence of magnesium sulfate. © 2012 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 27.
論文 |
論文
|
Yamada, Koji ; Somei, Masanori
概要:
Photoirradiation of 1-ethoxy-2-phenylindole in methanol and the reaction of 1-hydroxy-2-phenylindole with tosyl chloride
…
produced 6-ethoxy- and 6-tosyloxy-2-phenylindoles, respectively, as minor products. The latter was derived to 6-ethoxy-2-phenylindole. The structure is determined by direct comparison of the spectral data with those of the authentic 4-, 5-, 6-, and 7-ethoxy-2-phenylindoles whose syntheses are reported in detail. © 2012 The Japan Institute of Heterocyclic Chemistry.
続きを見る
|
||||||||||||||||||
| 28.
論文 |
論文
|
Ito, Tatsuro ; Sato, Jugo
概要:
TD-pairs of type II over C with shape 1, 2, ..., 2, 1 are classified by constructing all of them explicitly as certain s
…
ort of tensor product of two L-pairs in a matrix form involving 8 parameters in addition to the diameter. Six of the 8 parameters describe the eigenvalues and the other two give the zeros of the Drinfel'd polynomial. © 2014 Elsevier Inc.
続きを見る
|
||||||||||||||||||
| 29.
論文 |
論文
|
Nakata, Shunji ; Maeda, Ryoji ; Kawae, Takeshi ; Morimoto, Akiharu ; Shimizu, Tatsuo
概要:
A thin-film structure comprising Al2O3/Al-rich Al2O3/SiO2 was fabricated on Si substrate. We used radio-frequency magnet
…
ron co-sputtering with Al metal plates set on an Al2O3 target to fabricate the Al-rich Al2O 3 thin film, which is used as a charge storage layer for nonvolatile Al2O3 memory. We investigated the charge trapping characteristics of the film. When the applied voltage between the gate and the substrate is increased, the hysteresis window of capacitance-voltage (C-V) characteristics becomes larger, which is caused by the charge trapping in the film. For a fabricated Al-O capacitor structure, we clarified experimentally that the maximum capacitance in the C-V hysteresis agrees well with the series capacitance of insulators and that the minimum capacitance agrees well with the series capacitance of the semiconductor depletion layer and stacked insulator. When the Al content in the Al-rich Al2O3 is increased, a large charge trap density is obtained. When the Al content in the Al-O is changed from 40 to 58%, the charge trap density increases from 0 to 18 × 1018 cm-3, which is 2.6 times larger than that of the trap memory using SiN as the charge storage layer. The device structure would be promising for low-cost nonvolatile memory. © 2011 Elsevier B.V. All rights reserved.
続きを見る
|
||||||||||||||||||
| 30.
電子ブック |
ＥＢ
|
|
||||||||||||||||||
| 31.
電子ブック |
ＥＢ
|
|
||||||||||||||||||
| 32.
論文 |
論文
|
Nakano, Tomoyuki ; Shinsei, Naoki ; Ishida, Masahiro ; Tanaka, Yasunori ; Uesugi, Yoshihiko ; Ishijima, Tatsuo
概要:
This paper reports enhancement effect of N2/O2 gas addition in thermal plasma irradiation on the spallation phenomena fr
…
om polyamide-66 (PA66) specimens with water absorption. The polyamide materials are widely used, for example, in low-voltage circuit breakers and arcing horns as ablation material. In such devices, the polymer is often contacted with arc discharges to be ablated, which increases the arc current interruption ability of the circuit breakers. In our previous experiments, not only ablation but also spallation phenomena had been found to occur from PA66 specimens with water absorption by Ar thermal plasma irradiation. The present work was conducted to investigate effect of N2/O2 inclusion in the irradiating thermal plasma on the occurrence of spallation phenomena because the air circuit breaker was used in air. Relative oxygen admixture ratio to N2 was changed from 0% to 100% with a fixed heat flux irradiated to PA66 specimens. Results indicated that O2 inclusion effectively promotes the frequency of spallation particle ejection from PA66 specimen with 3 wt% water absorption. © 2013 IEEE.
続きを見る
|
||||||||||||||||||
| 33.
論文 |
論文
|
Tang, Ning ; Sato, Kousuke ; Tokuda, Takahiro ; Tatematsu, Michiya ; Hama, Hirotaka ; Suematsu, Chikako ; Kameda, Takayuki ; Toriba, Akira ; Hayakawa, Kazuichi
概要:
Airborne particulates were collected at a background site (Wajima Air Monitoring Station; WAMS) on the Noto Peninsula, J
…
apan from January 2006 to December 2007. 1-, 2-nitropyrenes (1-, 2-NPs) and 2-nitrofluoranthene (2-NFR), in the particulates were determined with a sensitive HPLC method with chemiluminescence detection. The average concentrations were higher in winter than in summer. A meteorological analysis indicated that the air samples collected in winter were transported mainly from Northeast China over the Japan Sea. Both the concentration ratios of 2-NFR to 1-NP and 1-NP to pyrene were similar to those in Shenyang in Northeast China which located along the air transportation route to WAMS, but not in Kanazawa which near WAMS. These results strongly suggest that most of the atmospheric 1-, 2-NPs and 2-NFR at WAMS in winter were long range transported from Northeast China. © 2014 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 34.
論文 |
論文
|
Yamaguchi, Junpei ; Sasaki, Motoko ; Sato, Yasunori ; Itatsu, Keita ; Harada, Kenichi ; Zen, Yoh ; Ikeda, Hiroko ; Nimura, Yuji ; Nagino, Masato ; Nakanuma, Yasuni
概要:
医薬保健研究域医学系<br />Polycomb group protein EZH2, frequently overexpressed in malignant tumors, is the catalytic subunit of p
…
olycomb repressive complex 2 (PRC2). PRC2 interacts with HDACs in transcriptional silencing and relates to tumor suppressor loss. We examined the expression of HDAC isoforms (HDAC 1 and 2) and EZH2, and evaluated the possible use of HDAC inhibitor suberoylanilide hydroxamic acid (SAHA) and EZH2 repressor for gallbladder carcinoma. We used 48 surgically resected gallbladders and cultures of human gallbladder epithelial cells (HGECs), gallbladder carcinoma (TGBC2TKB), and cholangiocarcinoma (HuCCT-1 and TFK-1) cell lines for examination. Immunohistochemically, EZH2 was overexpressed in gallbladder carcinoma, especially poorly differentiated carcinoma, but not in normal epithelium. In contrast, HDAC1/2 were expressed in both carcinoma and normal epithelium in vivo. This pattern was verified in cultured cells; EZH2 was highly expressed only in TGBC2TKB, whereas HDAC1/2 were expressed in HGECs and TGBC2TKB. Interestingly, SAHA treatment caused significant cell number decline in three carcinoma cells, and this effect was synergized with EZH2 siRNA treatment; however, HGECs were resistant to SAHA. In TGBC2TKB cells, the expression of EZH2 and HDAC1/2 were decreased by SAHA treatment, and p16INK4a, E-cadherin, and p21were simultaneously activated; however, no such findings were obtained in HGECs, suggesting that the effect of SAHA depends on the EZH2-mediated tumor suppressor loss. In conclusion, this study suggests a possible mechanism by which carcinoma cells but not normal cells are sensitive to SAHA and indicates the efficacy of this new anticancer agent in combination with EZH2 repression in gallbladder carcinoma. © 2009 Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 35.
論文 |
論文
|
Inoue, Daiki ; Kaido, Daisuke ; Yoshikawa, Yuta ; Minegishi, Mitsuyuki ; Matsumoto, Koichi ; Abe, Satoshi ; Murayama, Shigeyuki ; 松本, 宏一 ; 阿部, 聡
概要:
金沢大学理工研究域数物科学系<br />We have measured linear thermal expansion and magnetostriction of single crystal CeRu2Si2 that is we
…
ll known as a heavy fermion metamagnetic compound. Thermal expansion and magnetostriction along the a-axis (B | a) and the c-axis (B | c) were measured by the capacitive dilatometer at temperatures down to 12 mK and in magnetic fields up to 9 T. We observed a strong anisotropy between a and c axis. In addition, negative deviations from Landau-Fermi liquid behavior for thermal expansion and magnetostriction coefficients were found below 50 mK and 0.4 T indicating non Fermi liquid behavior. © 2015 The Authors. Published by Elsevier B.V.<br />出版社版
続きを見る
|
||||||||||||||||||
| 36.
論文 |
論文
|
Lan, Fei ; Misu, Hirofumi ; Chikamoto, Keita ; Takayama, Hiroaki ; Kikuchi, Akihiro ; Mohri, Kensuke ; Takata, Noboru ; Hayashi, Hiroto ; Matsuzawa-Nagata, Naoto ; Takeshita, Yumie ; Noda, Hiroyo ; Matsumoto, Yukako ; Ota, Tsuguhito ; Nagano, Toru ; Nakagen, Masatoshi ; Miyamoto, Ken-ichi ; Takatsuki, Kanako ; Seo, Toru ; Iwayama, Kaito ; Tokuyama, Kunpei ; Matsugo, Seiichi ; Tang, Hong ; Saito, Yoshiro ; Yamagoe, Satoshi ; Kaneko, Shuichi ; Takamura, Toshinari
概要:
Recent articles have reported an association between fatty liver disease and systemic insulin resistance in humans, but
…
the causal relationship remains unclear. The liver may contribute to muscle insulin resistance by releasing secretory proteins called hepatokines. Here we demonstrate that leukocyte cell-derived chemotaxin 2 (LECT2), an energy-sensing hepatokine, is a link between obesity and skeletal muscle insulin resistance. Circulating LECT2 positively correlated with the severity of both obesity and insulin resistance in humans. LECT2 expression was negatively regulated by starvation-sensing kinase adenosine monophosphate-activated protein kinase in H4IIEC hepatocytes. Genetic deletion of LECT2 in mice increased insulin sensitivity in the skeletal muscle. Treatment with recombinant LECT2 protein impaired insulin signaling via phosphorylation of Jun NH2-terminal kinase in C2C12 myocytes. These results demonstrate the involvement of LECT2 in glucose metabolism and suggest that LECT2 may be a therapeutic target for obesity-associated insulin resistance. © 2014 by the American Diabetes Association.
続きを見る
|
||||||||||||||||||
| 37.
図書 |
図書
|
37. Primes of the form x[2] + ny[2] : Fermat, class field theory, and complex multiplication. 2nd ed
David A. Cox
|
||||||||||||||||||
| 38.
論文 |
論文
|
Takakura, Hisashi ; Furuichi, Yasuro ; Yamada, Tatsuya ; Jue, Thomas ; Ojino, Minoru ; Hashimoto, Takeshi ; Iwase, Satoshi ; Hojo, Tatsuya ; Izawa, Tetsuya ; Masuda, Kazumi
概要:
At onset of muscle contraction, myoglobin (Mb) immediately releases its bound O2 to the mitochondria. Accordingly, intra
…
cellular O2 tension (PmbO2) markedly declines in order to increase muscle O2 uptake (mVO2). However, whether the change in PmbO2 during muscle contraction modulates mVO2 and whether the O2 release rate from Mb increases in endurance-trained muscles remain unclear. The purpose of this study was, therefore, to determine the effect of endurance training on O2 saturation of Mb (SmbO2) and PmbO2 kinetics during muscle contraction. Male Wistar rats were subjected to a 4-week swimming training (Tr group; 6 days per week, 30 min x 4 sets per day) with a weight load of 2% body mass. After the training period, deoxygenated Mb kinetics during muscle contraction were measured using near-infrared spectroscopy under hemoglobin-free medium perfusion. In the Tr group, the mVO2peak significantly increased by 32%. Although the PmbO2 during muscle contraction did not affect the increased mVO2 in endurance-trained muscle, the O2 release rate from Mb increased because of the increased Mb concentration and faster decremental rate in SmbO2 at the maximal twitch tension. These results suggest that the Mb dynamics during muscle contraction are contributing factors to faster VO2 kinetics in endurance-trained muscle. © 2015, Nature Publishing Group. All rights reserved.
続きを見る
|
||||||||||||||||||
| 39.
その他 |
その他
|
|
||||||||||||||||||
| 40.
論文 |
論文
|
Takakura, Hisashi ; Ojino, Minoru ; Jue, Thomas ; Yamada, Tatsuya ; Furuichi, Yasuro ; Hashimoto, Takeshi ; Iwase, Satoshi ; Masuda, Kazumi
概要:
Under acute hypoxic conditions, the muscle oxygen uptake (m (Formula presented.) O2) during exercise is reduced by the r
…
estriction in oxygen-supplied volume to the mitochondria within the peripheral tissue. This suggests the existence of a factor restricting the m (Formula presented.) O2 under hypoxic conditions at the peripheral tissue level. Therefore, this study set out to test the hypothesis that the restriction in m (Formula presented.) O2 is regulated by the net decrease in intracellular oxygen tension equilibrated with myoglobin oxygen saturation (∆PmbO2) during muscle contraction under hypoxic conditions. The hindlimb of male Wistar rats (8 weeks old, n = 5) was perfused with hemoglobin-free Krebs–Henseleit buffer equilibrated with three different fractions of O2 gas: 95.0%O2, 71.3%O2, and 47.5%O2. The deoxygenated myoglobin (Mb) kinetics during muscle contraction were measured under each oxygen condition with a near-infrared spectroscopy. The ∆[deoxy-Mb] kinetics were converted to oxygen saturation of myoglobin (SmbO2), and the PmbO2 was then calculated based on the SmbO2 and the O2 dissociation curve of the Mb. The SmbO2 and PmbO2 at rest decreased with the decrease in O2 supply, and the muscle contraction caused a further decrease in SmbO2 and PmbO2 under all O2 conditions. The net increase in m (Formula presented.) O2 from the muscle contraction (∆m (Formula presented.) O2) gradually decreased as the ∆PmbO2 decreased during muscle contraction. The results of this study suggest that ΔPmbO2 is a key determinant of the Δm (Formula presented.) O2. © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
続きを見る
|
||||||||||||||||||
| 41.
論文 |
論文
|
Begum, Zinnat A. ; Rahman, Ismail M. M. ; Sawai, Hikaru ; Tate, Yousuke ; Maki, Teruya ; Hasegawa, Hiroshi
概要:
The complex formation equilibria of Cr3+ and Fe3+ ions in aqueous solution with two biodegradable aminopolycarboxylate c
…
helants (dl-2-(2-carboxymethyl)nitrilotriacetic acid (GLDA) and 3-hydroxy-2,2'- iminodisuccinic acid (HIDS)) were investigated. The potentiometric data obtained at the constant ionic strengths (I) of (0.1 and 1.0) mol·dm-3 KCl and at (25 ± 0.1) °C was processed with the aid of the computer program HYPERQUAD 2008. The formation constants of the proton-chelant and metal-chelant (log KML) species (M = Fe3+ or Cr 3+; L = GLDA or HIDS) were determined, and the concentration distributions of complex species in solution were evaluated for both metal ions. In various pH conditions, the interaction between the chelants (L = GLDA or HIDS) and the metal ions (M = Fe3+ or Cr3+) leads to the formation of different complexes formulated as MH2L+, MHL, ML-, M(OH)L2-, and M(OH)2L3-. The log KML values at I = 0.1 mol·dm-3 KCl (T = (25 ± 0.1) °C) were 15.27 (log KFe-GLDA), 14.96 (log K Fe-HIDS), 13.77 (log KCr-GLDA), 12.67 (log K Cr-HIDS), and at I = 1.0 mol·dm-3 KCl (T = (25 ± 0.1) °C) were 14.79 (log KFe-GLDA), 14.34 (log K Fe-HIDS), 12.90 (log KCr-GLDA), 12.09 (log K Cr-HIDS). The conditional stability constants (log K'ML) of the ML complexes were calculated in terms of pH in the range of 2 to 12 and compared with the same for EDTA and other biodegradable chelants (NTA and EDDS). © 2012 American Chemical Society.
続きを見る
|
||||||||||||||||||
| 42.
論文 |
論文
|
Tanaka, Shigeo M.
概要:
In bone cells, intracellular Ca2+ (iCa2+) functions as a second messenger in the mechanotransduction pathway. Its responses to mechanical stimulation can be observed microscopically on a rigid flat surface with a Ca2+ fluorescent
…
indicator, generally, under fluid flow. However, bone cells are physiologically exposed to dynamic loading accompanied by bone matrix deformation. In this situation, microscopic methods of observing iCa2+ responses cannot be used because of the loss of focus or movement of cells out of the observation area. The purpose of this study was to develop a compact optical device for the observation of iCa2+ responses of osteoblasts to dynamic loadings accompanied by substrate deformation. This system comprised four light emitting diodes (LEDs) and a photodetector (PD) placed underneath a culture chamber, specifically designed for tissue-level iCa2+ observations. This device was used to study the frequency dependence of iCa2+ responses of osteoblasts to dynamic loading. MC3T3-E1 osteoblasts were cultured three-dimensionally in a collagen sponge scaffold with the fluorescent Ca2+ indicator Fluo-4 AM and mechanically stimulated by a 0.2% deformation of the sponge at 0.2, 2, or 20 Hz for 150 s. Our device succeeded in detecting temporal changes in the intensity of emitted fluorescent light, showing a frequency-dependent increase in fluorescence intensity. This device may contribute to further understanding of the mechanosensing and mechanotransduction mechanisms in bone under near-physiological conditions.
続きを見る
|
||||||||||||||||||
| 43.
論文 |
論文
|
Izumi, Kouji ; Mizokami, Atsushi ; Lin, Hsiu-Ping ; Ho, Hui-Min ; Iwamoto, Hiroaki ; Maolake, Aerken ; Natsagdorj, Ariunbold ; Kitagawa, Yasuhide ; Kadono, Yoshifumi ; Miyamoto, Hiroshi ; Huang, Chiung-Kuei ; Namiki, Mikio ; Lin, Wen-Jye
概要:
Prostate-specific antigen (PSA) is regarded as the most sensitive biomarker for prostate cancer. Although androgen/andro
…
gen receptor (AR) signaling promotes prostate cancer progression, suppression of AR signaling induces chemokine (CC motif) ligand 2 (CCL2), which enables prostate cancer cells to gain metastatic potential. AR-controlled PSA alone may be an unreliable biomarker for patients receiving androgen deprivation therapy. Therefore, we investigated the validity of CCL2 as a complementary biomarker to PSA for prostate cancer. Our in vitro approach of enriching for prostate cancer cells with higher migration potential showed that CCL2 activated cellular migration. Importantly, we found that CCL2 levels were significantly different between men (n = 379) with and without prostate cancer. Patients with CCL2 ≥ 320 pg/mL had worse overall survival and prostate cancer -specific survival than those with CCL2 < 320 pg/mL. A novel risk classification was developed according to the risk factors CCL2 ≥ 320 pg/mL and PSA ≥ 100 ng/mL, and scores of 2, 1, and 0 were defined as poor, intermediate, and good risk, respectively, and clearly distinguished patient outcomes. CCL2 may serve as a novel biomarker for prostate cancer. The novel risk classification based on combining CCL2 and PSA is more reliable than using either alone.
続きを見る
|
||||||||||||||||||
| 44.
論文 |
論文
|
Fukuyoshi, Shuichi ; Ooi, Takashi ; Nakagaki, Ryoichi
概要:
The crystal structure of 2-[1-(2,4-dinitrophenyl)ethyl]-1,10-phenanthroline was determined by X-ray crystallography. The
…
compound crystallizes in a monoclinic system and was characterized thus: P21/n, a = 15.477(2), b = 5.1818(6), c = 21.754(2)Å, β = 96.295(3)°, Z = 4, V = 1734.12 Å3. The crystal structure was solved by direct methods and refined by full-matrix least-squares on F2 to final values of R = 0.0600.
続きを見る
|
||||||||||||||||||
| 45.
論文 |
論文
|
Ebisu, Haruka ; Iwai-Takekoshi, Lena ; Fujita-Jimbo, Eriko ; Momoi, Takashi ; Kawasaki, Hiroshi
概要:
The molecular mechanisms underlying the formation of the thalamus during development have been investigated intensively.
…
Although transcription factors distinguishing the thalamic primordium from adjacent brain structures have been uncovered, those involved in patterning inside the thalamus are largely unclear. Here, we show that Foxp2, a member of the forkhead transcription factor family, regulates thalamic patterning during development. We found a graded expression pattern of Foxp2 in the thalamic primordium of the mouse embryo. The expression levels of Foxp2 were high in the posterior region and low in the anterior region of the thalamic primordium. In Foxp2 (R552H) knockin mice, which have a missense loss-of-function mutation in the forkhead domain of Foxp2, thalamic nuclei of the posterior region of the thalamus were shrunken, while those of the intermediate region were expanded. Consistently, Foxp2 (R552H) knockin mice showed changes in thalamocortical projection patterns. Our results uncovered important roles of Foxp2 in thalamic patterning and thalamocortical projections during development. © 2017 Author.<br />Embargo Period 12 months
続きを見る
|
||||||||||||||||||
| 46.
論文 |
論文
|
Sato, Hiroshi ; Takino, Takahisa
概要:
Membrane-type matrix metalloproteinase-1 (MT1-MMP) mediates cleavage of not only MMP-2/gelatinase A for activation, but
…
also a variety of substrates including type I collagen (reviewed in Cancer Sci 2005; 96: 212-7). MMP-2 activation involves tissue inhibitor of MMP (TIMP)-2 as a bridging molecule between MT1-MMP and pro-MMP-2. Thus, net activity of MT1-MMP and MMP-2 is regulated in a complex manner depending on TIMP-2 concentration. During invasive growth of tumor cells in type I collagen matrix, MT1-MMP initiates denaturation of collagen into gelatin, which is subsequently digested further by MMP-2 adjacent to MT1-MMP. Coordinate action of MT1-MMP and MMP-2 may facilitate pericellular proteolysis, and enhance not only tumor invasion/migration but also cell growth. Tetraspanins as binding proteins of MT1-MMP regulate MT1-MMP subcellular localization and compartmentalization, leading to efficient MMP-2 activation and proteolysis coupled with cellular function. © 2010 Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 47.
論文 |
論文
|
Tajiri, Ryosuke ; Ooi, Akishi ; Fujimura, Takashi ; Dobashi, Yoh ; Oyama, Takeru ; Nakamura, Ritsuko ; Ikeda, Hiroko
概要:
A humanized monoclonal antibody against ERBB2 is used in neoadjuvant therapy for patients with gastric cancer. A critica
…
l factor in determining patient eligibility and predicting outcomes of this therapy is the intratumoral heterogeneity of ERBB2 amplification in gastric adenocarcinomas. The aims of this study are to assess the underlying mechanisms of intratumoral heterogeneity of ERBB2 amplification; to characterize the diversity of coamplified oncogenes such as EGFR, FGFR2, MET, MYC, CCND1, and MDM2; and to examine the usefulness of multiple ligation-dependent probe amplification (MLPA) in the semicomprehensive detection of these gene amplifications. A combined analysis of immunohistochemistry and fluorescence in situ hybridization revealed ERBB2-amplified cancer cells in 51 of 475 formalin-fixed, paraffin-embedded gastric adenocarcinomas. The fraction of amplification-positive cells in each tumor ranged from less than 10% to almost 100%. Intratumoral heterogeneity of ERBB2 amplification, defined as less than 50% of cancer cells positive for ERBB2 amplification, was found in 41% (21/51) of ERBB2-amplified tumors. The combined analysis of MLPA and fluorescence in situ hybridization revealed that ERBB2 was coamplified with EGFR in 7 tumors, FGFR2 in 1 tumor, and FGFR2 and MET in 1 tumor; however, the respective genes were amplified in mutually exclusive cells. Coamplified ERBB2 and MYC coexisted within single nuclei in 4 tumors, and one of these cases had suspected coamplification in the same amplicon of ERBB2 with MYC. In conclusion, the amplification status of ERBB2 and other genes can be obtained semicomprehensively by MLPA and could be useful to plan individualized molecularly targeted therapy against gastric cancers. © 2014 Elsevier Inc.
続きを見る
|
||||||||||||||||||
| 48.
図書 |
図書
|
Stéphanie Porchy-Simon
|
||||||||||||||||||
| 49.
図書 |
図書
|
Walker, Grant ; Wood, Reginald M. W.
|
||||||||||||||||||
| 50.
論文 |
論文
|
Takahashi, Kana ; Iwamoto, Ryoji ; Sakata, Ryo ; Soeta, Takahiro ; Inomata, Katsuhiko ; Ukaji, Yutaka
概要:
Oxidation of 4-methylpyrrole-2-carboxylates with DDQ in the presence of a glycol proceeded smoothly on the methyl group
…
at the C4 position regioselectively to afford the corresponding pyrrole-2,4-dicarboxylates directly. Direct oxidation of a methyl group of 2,4,6-trimethylphenol and 3-methyl-9H-carbazole into carboxylates was also demonstrated. © 2012 The Japan Institute of Heterocyclic Chemistry.
続きを見る
|
||||||||||||||||||
| 51.
論文 |
論文
|
Sakata, Ryo ; Iwamoto, Ryoji ; Fujinami, Shuhei ; Ukaji, Yutaka ; Inomata, Katsuhiko
概要:
金沢大学理工研究域物質化学系<br />t-Butyl 3,4-dialkyl-1H-pyrrole-2-carboxylates were oxidized with ochloranil in the presence of MeOH
…
to afford the corresponding 5-methoxypyrrolin-2-one derivatives. The resulting 5-methoxypyrrolin-2-one was reacted with various nucleophiles under acidic conditions to afford the functionalized pyrrolinone derivatives in good yields. © The Japan Institute of Heterocyclic Chemistry.
続きを見る
|
||||||||||||||||||
| 52.
図書 |
図書
|
Tao Le ... [et al.]
|
||||||||||||||||||
| 53.
論文 |
論文
|
Sugimoto, Naotoshi ; Ohta, Kunio ; Saito, Takekatsu ; Nakayama, Yuko ; Nakamura, Taichi ; Maeda, Akio ; Yachie, Akihiro
概要:
Kawasaki disease is an acute illness of early childhood that is characterized by prolonged fever and vasculitis of unkno
…
wn pathogenesis. Lactobacillus casei cell wall extract (LCWE)-induced vasculitis in mice is a well-validated model of Kawasaki disease. In the nervous system, glial cells play an important role in fever development. This study investigated whether LCWE directly stimulates glial cells, resulting in the induction of cyclooxygenase-2 (COX2), which is required for prostaglandin synthesis and fever development. We found that LCWE induced COX2 expression and activated the nuclear factor-κB signaling pathway in rat B92 glial cells, but Toll-like receptor-2, which is one of the receptors for LCWE, could not be detected in the cells. These results suggest that LCWE activates the nuclear factor-κB signaling pathway and induces COX2 in rat B92 glial cells through another LCWE receptor other than Toll-like receptor-2. © 2012.
続きを見る
|
||||||||||||||||||
| 54.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 55.
その他 |
その他
|
Hori, Osamu
|
||||||||||||||||||
| 56.
図書 |
|
Michael R. Green, Joseph Sambrook
|
||||||||||||||||||
| 57.
論文 |
論文
|
Yamada, Fumio ; Goto, Aya ; Hasegawa, Masakazu ; Kobayashi, Kensuke ; Somei, Masanori ; 山田, 文夫 ; 染井, 正徳
概要:
金沢大学医薬保健研究域薬学系<br />Various nucleophiles, such as indole, 1,2,3-trimethoxybenzene, anisole, phenol, and pyrrole, reacted
…
with 1-hydroxy-Nb-trifluoroacetyltryptamine under the presence of mesyl chloride to give novel series of (3a,8acis)- 1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indoles having a substituent at the 3aposition. Their structures and by-products were strictly determined. © 2017 The Japan Institute of Heterocyclic Chemistry.<br />Embargo Period 12 months
続きを見る
|
||||||||||||||||||
| 58.
論文 |
論文
|
Ogai, Kazuhiro ; Nakatani, Kumi ; Hisano, Suguru ; Sugitani, Kayo ; Koriyama, Yoshiki ; Kato, Satoru
概要:
The sex-determining region Y-box 2 (Sox2) is related not only to pluripotency, but also to cell proliferation. Zebrafish
…
can regain their motor function after spinal cord injury (SCI). Following SCI, new motor neurons are produced from proliferating ependymal cells. Here, we investigated the expression and function of Sox2 after SCI in zebrafish. Sox2 was upregulated as early as 1 day post-lesion (dpl) in ependymal cells, which was followed by cell proliferation. Sox2 knockdown significantly decreased the number of proliferating cells at 5 dpl. The results of this study suggest a role of Sox2 as one of the proliferation initiators in ependymal cells after SCI. © 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society.<br />12 months Embargo Period
続きを見る
|
||||||||||||||||||
| 59.
論文 |
論文
|
Ogai, Kazuhiro ; Nakatani, Kumi ; Hisano, Suguru ; Sugitani, Kayo ; Koriyama, Yoshiki ; Kato, Satoru
概要:
The sex-determining region Y-box 2 (Sox2) is related not only to pluripotency, but also to cell proliferation. Zebrafish
…
can regain their motor function after spinal cord injury (SCI). Following SCI, new motor neurons are produced from proliferating ependymal cells. Here, we investigated the expression and function of Sox2 after SCI in zebrafish. Sox2 was upregulated as early as 1 day post-lesion (dpl) in ependymal cells, which was followed by cell proliferation. Sox2 knockdown significantly decreased the number of proliferating cells at 5dpl. The results of this study suggest a role of Sox2 as one of the proliferation initiators in ependymal cells after SCI. Copyright © 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
続きを見る
|
||||||||||||||||||
| 60.
論文 |
論文
|
Rahman, Ismail M. M. ; Furusho, Yoshiaki ; Begum, Zinnat A. ; Sato, Rika ; Okumura, Hiroshi ; Honda, Hiroko ; Hasegawa, Hiroshi
概要:
Lead (+2) was selectively adsorbed on a solid phase extraction (SPE) gel (molecular recognition technology, MRT), quanti
…
tatively extracted, and spectrophotometrically determined as the Pb(II)-PAR (4-(2-pyridylazo)- resorcinol) complex. The linear range was 0.01 to 0.75 mg L-1 and the detection limit was 6.4 μg L-1. The MRT-SPE allows selective Pb(II) extraction from complex ion-rich matrices, which is difficult with other techniques. Interference from common matrix ions such as Fe2+, Ni2+, Cu2+ or Co2+ is minimized. [Figure not available: see fulltext.] © 2013 Versita Warsaw and Springer-Verlag Wien.
続きを見る
|
||||||||||||||||||
| 61.
論文 |
論文
|
Aki, Sho ; Yoshioka, Kazuaki ; Okamoto, Yasuo ; Takuwa, Noriko ; Takuwa, Yoh
概要:
We have recently demonstrated that the PI3K class II-α isoform (PI3K-C2α), which generates phosphatidylinositol 3-phosph
…
ate and phosphatidylinositol 3,4-bisphosphates, plays crucial roles in angiogenesis, by analyzing PI3K-C2α knock-out mice. The PI3K-C2α actions are mediated at least in part through its participation in the internalization of VEGF receptor-2 and sphingosine-1-phosphate receptor S1P1 and thereby their signaling on endosomes. TGFβ, which is also an essential angiogenic factor, signals via the serine/threonine kinase receptor complex to induce phosphorylation of Smad2 and Smad3 (Smad2/3). SARA (Smad anchor for receptor activation) protein, which is localized in early endosomes through its FYVE domain, is required for Smad2/3 signaling. In the present study, we showed that PI3K-C2α knockdown nearly completely abolished TGFβ1-induced phosphorylation and nuclear translocation of Smad2/3 in vascular endothelial cells (ECs). PI3K-C2α was necessary for TGFβ-induced increase in phosphatidylinositol 3,4-bisphosphates in the plasma membrane and TGFβ receptor internalization into the SARA-containing early endosomes, but not for phosphatidylinositol 3-phosphate enrichment or localization of SARA in the early endosomes. PI3K-C2α was also required for TGFβ receptor-mediated formation of SARA-Smad2/3 complex. Inhibition of dynamin, which is required for the clathrin-dependent receptor endocytosis, suppressed both TGFβ receptor internalization and Smad2/3 phosphorylation. TGFβ1 stimulated Smad-dependent VEGF-A expression, VEGF receptor-mediated EC migration, and capillary-like tube formation, which were all abolished by either PI3K-C2α knockdown or a dynamin inhibitor. Finally, TGFβ1-induced microvessel formation in Matrigel plugs was greatly attenuated in EC-specific PI3K-C2α-deleted mice. These observations indicate that PI3K-C2α plays the pivotal role in TGFβ receptor endocytosis and thereby Smad2/3 signaling, participating in angiogenic actions of TGFβ. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
続きを見る
|
||||||||||||||||||
| 62.
論文 |
論文
|
Nakano, Tomoyuki ; Shinsei, Naoki ; Ishida, Masahiro ; Tanaka, Yasunori ; Uesugi, Yoshihiko ; Ishijima, Tatsuo
概要:
This paper reports enhancement effect of N2/O2 gas addition in thermal plasma irradiation on the spallation phenomena fr
…
om polyamide-66 (PA66) specimens with water absorption. The polyamide materials are widely used, for example, in low-voltage circuit breakers and arcing horns as ablation material. In such devices, the polymer is often contacted with arc discharges to be ablated, which increases the arc current interruption ability of the circuit breakers. In our previous experiments, not only ablation but also spallation phenomena had been found to occur from PA66 specimens with water absorption by Ar thermal plasma irradiation. The present work was conducted to investigate effect of N2/O2 inclusion in the irradiating thermal plasma on the occurrence of spallation phenomena because the air circuit breaker was used in air. Relative oxygen admixture ratio to N2 was changed from 0% to 100% with a fixed heat flux irradiated to PA66 specimens. Results indicated that O2 inclusion effectively promotes the frequency of spallation particle ejection from PA66 specimen with 3 wt% water absorption. © 2013 IEEE.
続きを見る
|
||||||||||||||||||
| 63.
論文 |
論文
|
Tamura, Masayoshi ; Mochizuki, Naoki ; Nagatomi, Yasushi ; Harayama, Koichi ; Toriba, Akira ; Hayakawa, Kazuichi
概要:
Three compounds, hypothesized as fumonisin A1 (FA1), fumonisin A2 (FA2), and fumonisin A3 (FA3), were detected in a corn
…
sample contaminated with mycotoxins by high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS). One of them has been identified as FA1 synthesized by the acetylation of fumonisin B1 (FB1), and established a method for its quantification. Herein, we identified the two remaining compounds as FA2 and FA3, which were acetylated fumonisin B2 (FB2) and fumonisin B3 (FB3), respectively. Moreover, we examined a method for the simultaneous analysis of FA1, FA2, FA3, FB1, FB2, and FB 3. The corn samples were prepared by extraction using a QuEChERS kit and purification using a multifunctional cartridge. The linearity, recovery, repeatability, limit of detection, and limit of quantification of the method were >0.99, 82.9%–104.6%, 3.7%–9.5%, 0.02–0.60 μg/kg, and 0.05–1.98 μg/kg, respectively. The simultaneous analysis of the six fumonisins revealed that FA1, FA2, and FA3 were present in all corn samples contaminated with FB1, FB2, and FB 3. The results suggested that corn marketed for consumption can be considered as being contaminated with both the fumonisin B-series and with fumonisin A-series. This report presents the first identification and quantification of FA1, FA2, and FA3 in corn samples. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
続きを見る
|
||||||||||||||||||
| 64.
論文 |
論文
|
Takeichi, Toshiaki ; Takarada-Iemata, Mika ; Hashida, Koji ; Sudo, Hirofumi ; Okuda, Tomohiko ; Kokame, Koichi ; Hatano, Taku ; Takanashi, Masashi ; Funabe, Sayaka ; Hattori, Nobutaka ; Kitamura, Osamu ; Kitao, Yasuko ; Hori, Osamu
概要:
N-myc downstream-regulated gene 2 (Ndrg2) is a differentiation- and stress-associated molecule predominantly expressed i
…
n astrocytes in the central nervous system (CNS). To study the expression and possible role of Ndrg2 in quiescent and activated astrocytes, mice were administrated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropypridine (MPTP), a Parkinson disease (PD)-related neurotoxin which causes both neurodegeneration and glial activation. Immunohistological analysis revealed that Ndrg2 was highly expressed in both types of astrocytes, but less so in astrocytes during the early process of activation. Ndrg2 was also expressed in astrocyte-like cells, but not in neurons, in human brains from PD and Cortico-basal degeneration (CBD) patients. In cultured astrocytes, gene silencing of Ndrg2 significantly enhanced the numbers of 5-bromo-2′-deoxy-uridine (BrdU)-incorporated and proliferating cell nuclear antigen (PCNA)-positive cells, and reduced the length of cell processes and the amount of F-actin. In contrast, adenovirus-mediated overexpression of Ndrg2 significantly reduced the numbers of BrdU-incorporated and PCNA-positive cells, and enhanced the amount of F-actin. Fractionation and immunocytochemical analysis further revealed that Ndrg2 was located in different cellular fractions including the cytosol and cell surface membranes. These results suggest that Ndrg2 may regulate astroglial activation through the suppression of cell proliferation and stabilization of cell morphology. © 2011 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 65.
その他 |
その他
|
|
||||||||||||||||||
| 66.
その他 |
その他
|
|
||||||||||||||||||
| 67.
論文 |
論文
|
Juanjuan, Zhao ; Okamoto, Yasuo ; Takuwa, Yoh
概要:
The lysophospholipid mediator sphingosine-1-phosphate (S1P) exerts diverse biological activities including the regulatio
…
n of leukocyte migration and vascular barrier integrity, suggesting that S1P signaling could be involved in inflammatory fibrotic diseases. Pulmonary fibrosis is a devastating disease characterized by fibroblast accumulation and extracellular matrix deposition in lungs, and bleomycin–induced pulmonary fibrosis is the most widely used experimental model. We studied the effects of the S1P–specific receptor S1P2 on the phenotypes of lung fibroblasts isolated from bleomycin- and saline-administered wild-type and S1P2–null (S1pr2-/-) mice. The lung fibroblasts from bleomycin-administered wild-type and S1pr2-/- mice failed to proliferate in the presence of serum, unlike fibroblasts from saline-administered mice. The fibroblasts from bleomycin-administered mice also showed the enlarged and flattened morphology compared with fibroblasts from control mice. Bleomycin administration increased the protein expression of the cell cycle inhibitor p16INK4a in fibroblasts and the number of senescence–associated β-galactosidase (SA-β-gal)-positive fibroblasts. In S1pr2-/- fibroblasts, bleomycin administration-induced increases in p16INK4a protein expression and SA–β-gal–positive cells were augmented. Furthermore, bleomycin increased mRNA expression of interleukin-6 and matrix metalloproteinases in S1pr2-/- fibroblasts compared with wild-type fibroblasts. In addition, the activation of Akt in response to platelet–derived growth factor and S1P was enhanced in S1pr2-/- fibroblasts compared with wild-type fibroblasts. These results indicate that S1P2 deletion enhances bleomycin administration–induced cellular senescence of lung fibroblasts, which may lead to inhibition of lung fibrosis through the mechanisms involving increased matrix metalloproteinases expression. Thus, S1P2 may be a novel therapeutic target for lung fibrosis.
続きを見る
|
||||||||||||||||||
| 68.
論文 |
論文
|
Mohri, Takuya ; Nakajima, Miki ; Fukami, Tatsuki ; Takamiya, Masataka ; Aoki, Yasuhiro ; Yokoi, Tsuyoshi
概要:
金沢大学医薬保健研究域薬学系<br />Human CYP2E1 is one of the pharmacologically and toxicologically important cytochrome P450 isoforms.
…
Earlier studies have reported that the CYP2E1 expression is extensively regulated by post-transcriptional and post-translational mechanisms, but the molecular basis remains unclear. In the present study, we examined the possibility that microRNA may be involved in the post-transcriptional regulation of human CYP2E1. In silico analysis identified a potential recognition element of miR-378 (MRE378) in the 3'-untranslated region (UTR) of human CYP2E1 mRNA. Luciferase assays using HEK293 cells revealed that the reporter activity of the plasmid containing the MRE378 was decreased by co-transfection of precursor miR-378, indicating that miR-378 functionally recognized the MRE378. We established two HEK293 cell lines stably expressing human CYP2E1 including or excluding 3'-UTR. When the precursor miR-378 was transfected into the cells expressing human CYP2E1 including 3'-UTR, the CYP2E1 protein level and chlorzoxazone 6-hydroxylase activity were significantly decreased, but were not in the cells expressing CYP2E1 excluding 3'-UTR. In both cell lines, the CYP2E1 mRNA levels were decreased by overexpression of miR-378, but miR-378 did not affect the stability of CYP2E1 mRNA. In a panel of 25 human livers, no positive correlation was observed between the CYP2E1 protein and CYP2E1 mRNA levels, supporting the post-transcriptional regulation. Interestingly, the miR-378 levels were inversely correlated with the CYP2E1 protein levels and the translational efficiency of CYP2E1. In conclusion, we found that human CYP2E1 expression is regulated by miR-378, mainly via translational repression. This study could provide new insight into the unsolved mechanism of the post-transcriptional regulation of CYP2E1. Copyright 2009 Elsevier Inc. All rights reserved.
続きを見る
|
||||||||||||||||||
| 69.
論文 |
論文
|
早川, 和一
|
||||||||||||||||||
| 70.
電子ブック |
ＥＢ
|
|
||||||||||||||||||
| 71.
電子ブック |
ＥＢ
|
|
||||||||||||||||||
| 72.
図書 |
図書
|
translated and annotated by Greg Bailey
目次情報:
|
||||||||||||||||||
| 73.
論文 |
論文
|
Sumino, Ayumi ; Sumikama, Takashi ; Uchihashi, T. ; Oki, S. ; 角野, 歩 ; 炭竈, 享司
概要:
金沢大学ナノ生命科学研究所<br />Agitoxin-2 (AgTx2) from scorpion venom is a potent blocker of K+ channels. The docking model has been
…
elucidated, but it remains unclear whether binding dynamics are described by a two-state model (AgTx2-bound and AgTx2-unbound) or a more complicated mechanism, such as induced fit or conformational selection. Here, we observed the binding dynamics of AgTx2 to the KcsA channel using high-speed atomic force microscopy. From images of repeated binding and dissociation of AgTx2 to the channel, single-molecule kinetic analyses revealed that the affinity of the channel for AgTx2 increased during persistent binding and decreased during persistent dissociation. We propose a four-state model, including high- and low-affinity states of the channel, with relevant rate constants. An induced-fit pathway was dominant and accelerated binding by 400 times. This is the first analytical imaging of scorpion toxin binding in real time, which is applicable to various biological dynamics including channel ligands, DNA-modifier proteins, and antigen-antibody complexes.
続きを見る
|
||||||||||||||||||
| 74.
論文 |
論文
|
Tsuji, Takahiro ; Higashida, Chiharu ; Aoki, Yoshihiko ; Mohammad, Saharul Islam ; Dohmoto, Mitsuko ; Higashida, Haruhiro
概要:
In collaboration with Marshall Nirenberg, we performed in vivo RNA interference (RNAi) genome-wide screening in Drosophi
…
la embryos. Pebble has been shown to be involved in Drosophila neuronal development. We have also reported that depletion of Ect2, a mammalian ortholog of Pebble, induces differentiation in NG108-15 neuronal cells. However, the precise role of Ect2 in neuronal development has yet to be studied. Here, we confirmed in PC12 pheochromocytoma cells that inhibition of Ect2 expression by RNAi stimulated neurite outgrowth, and in the mouse embryonic cortex that Ect2 was accumulated throughout the ventricular and subventricular zones with neuronal progenitor cells. Next, the effects of Ect2 depletion were studied in primary cultures of mouse embryonic cortical neurons: Loss of Ect2 did not affect the differentiation stages of neuritogenesis, the number of neurites, or axon length, while the numbers of growth cones and growth cone-like structures were increased. Taken together, our results suggest that Ect2 contributes to neuronal morphological differentiation through regulation of growth cone dynamics. © 2011 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 75.
論文 |
論文
|
Ohashi, Masashi ; Miyagawa, Hidenori ; Oomi, Gendo ; Nakano, Tomohito ; Satoh, Isamu ; Komatsubara, Takemi
概要:
The magnetic and thermal properties of Ce1−xErxAl2 compounds have been studied using electrical resistivity and magnetic
…
susceptibility measurements. The magnetic ordering temperature continuously changes with a change from antiferro-magnetism (CeAl2) to ferro-magnetism (ErAl2) appears. The magnetic ordering temperature is continuously changed as a function of Er concentration x. A spin glass behavior was found between x = 0.08 and 0.4. The magnetic phase diagram was compiled as a function of x.<br />Embargo Period 12 months
続きを見る
|
||||||||||||||||||
| 76.
論文 |
論文
|
Matsuo, Junichi ; Hattori, Yu ; Hashizume, Mio ; Ishibashi, Hiroyuki
概要:
金沢大学医薬保健研究域薬学系<br />Aliphatic ketones were reduced to the corresponding secondary alcohols by using anti-1,3-diol and a
…
catalytic amount of 2,4-dinitrobenzenesulfonic acid (DNBSA) in benzene at reflux. Addition of 1-octanethiol in that media improved the efficiency of the reduction. Asymmetric reduction of aliphatic ketones was performed by using chiral anti-pentane-2,4-diol, and highly asymmetric induction (up to >99% ee) was observed in the reduction of tert-alkyl ketones. Asymmetric reduction of acyl silanes using chiral anti-pentane-2,4-diol and DNBSA proceeded efficiently in the absence of octanethiol and the corresponding α-silyl alcohols were obtained in high yields with high ees. © 2010 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 77.
論文 |
論文
|
Matsuo, Jun-ichi ; Hattori, Yu ; Hashizume, Mio ; Ishibashi, Hiroyuki
概要:
金沢大学医薬保健研究域薬学系<br />Aliphatic ketones were reduced to the corresponding secondary alcohols by using anti-1,3-diol and a
…
catalytic amount of 2,4-dinitrobenzenesulfonic acid (DNBSA) in benzene at reflux. Addition of 1-octanethiol in that media improved the efficiency of the reduction. Asymmetric reduction of aliphatic ketones was performed by using chiral anti-pentane-2,4-diol, and highly asymmetric induction (up to >99% ee) was observed in the reduction of tert-alkyl ketones. Asymmetric reduction of acyl silanes using chiral anti-pentane-2,4-diol and DNBSA proceeded efficiently in the absence of octanethiol and the corresponding α-silyl alcohols were obtained in high yields with high ees. © 2010.
続きを見る
|
||||||||||||||||||
| 78.
電子ブック |
ＥＢ
|
|
||||||||||||||||||
| 79.
論文 |
論文
|
Kawano, Mizuki ; Kiuchi, Takaaki ; Matsuo, Jun-ichi ; Ishibashi, Hiroyuki
概要:
Cyclobutanones bearing an alkyne-cobalt complex at their 3-positions reacted with aldehydes to give formal [4+2] cycload
…
ducts by using tin(IV) chloride as a Lewis acid. Highly substituted tetrahydropyrone derivatives were stereoselectively prepared by this method. © 2011 Elsevier Ltd. All rights reserved.
続きを見る
|
||||||||||||||||||
| 80.
図書 |
図書
|
Tao Le ... [et al.]
|
||||||||||||||||||
| 81.
論文 |
論文
|
Oki, Shinya ; Sone, Kenbun ; Oda, Katsutoshi ; Hamamoto, Ryuji ; Ikemura, Masako ; Maeda, Daichi ; Takeuchi, Makoto ; Tanikawa, Michihiro ; Mori-Uchino, Mayuyo ; Nagasaka, Kazunori ; Miyasaka, Aki ; Kashiyama, Tomoko ; Ikeda, Yuji ; Arimoto, Takahide ; Kuramoto, Hiroyuki ; Wada-Hiraike, Osamu ; Kawana, Kei ; Fukayama, Masashi ; Osuga, Yutaka ; Fujii, Tomoyuki ; 前田, 大地
概要:
金沢大学医薬保健研究域医学系<br />The histone methyltransferase EZH2, a key epigenetic modifier, is known to be associated with human
…
tumorigenesis. However, the physiological importance of EZH2 and its clinical relevance in endometrial cancer remain unclear. Hence, in the present study, we investigated the expression and function of EZH2 in endometrial cancer. In a quantitative real-time PCR analysis of 11 endometrial cancer cell lines and 52 clinical endometrial cancer specimens, EZH2 was significantly overexpressed in cancer cells and tissues compared to that in corresponding normal control cells and tissues. Kaplan-Meier survival analysis using data of the TCGA RNA-seq database and tissue microarrays (TMAs) indicated that EZH2 overexpression is associated with endometrial cancer prognosis. In addition, knockdown of EZH2 using specific siRNAs resulted in growth suppression and apoptosis induction of endometrial cancer cells, accompanied by attenuation of H3K27 trimethylation. Consistent with these results, treatment with GSK126, a specific EZH2 inhibitor, suppressed endometrial cancer cell growth and decreased the number of cancer cell colonies. Furthermore, GSK126 showed additive effects with doxorubicin or cisplatin, which are conventional drugs for treatment of endometrial cancer. Further studies should explore the therapeutic potential of inhibiting EZH2 in patients with endometrial cancer. © Oki et al.
続きを見る
|
||||||||||||||||||
| 82.
論文 |
論文
|
Higashida, Haruhiro
概要:
子どものこころの発達研究センター<br />Oxytocin (OT) is released into the brain from the cell soma, axons, and dendrites of neurosecretor
…
y cells in the hypothalamus. Locally released OT can activate OT receptors, form inositol-1,4,5-trisphosphate and elevate intracellular free calcium (Ca2+) concentrations [(Ca2+)i] in self and neighboring neurons in the hypothalamus, resulting in further OT release: i.e., autocrine or paracrine systems of OT-induced OT release. CD38-dependent cyclic ADP-ribose (cADPR) is also involved in this autoregulation by elevating [Ca2+]i via Ca2+ mobilization through ryanodine receptors on intracellular Ca2+ pools that are sensitive to both Ca2+ and cADPR. In addition, it has recently been reported that heat stimulation and hyperthermia enhance [Ca2+]i increases by Ca2+ influx, probably through TRPM2 cation channels, suggesting that cADPR and TRPM2 molecules act as Ca2+ signal amplifiers. Thus, OT release is not simply due to depolarization–secretion coupling. Both of these molecules play critical roles not only during labor and milk ejection in reproductive females, but also during social behavior in daily life in both genders. This was clearly demonstrated in CD38 knockout mice in that social behavior was impaired by reduction of [Ca2+]i elevation and subsequent OT secretion. Evidence for the associations of CD38 with social behavior and psychiatric disorder is discussed, especially in subjects with autism spectrum disorder. © 2015 The Author(s)<br />Embargo Period 12 months
続きを見る
|
||||||||||||||||||
| 83.
論文 |
論文
|
Takarada-Iemata, Mika ; Kezuka, Dai ; Takeichi, Toshiaki ; Ikawa, Masahito ; Hattori, Tsuyoshi ; Kitao, Yasuko ; Hori, Osamu
概要:
N-myc downstream-regulated gene 2 (Ndrg2) is a differentiation- and stress-associated molecule predominantly expressed i
…
n astrocytes in the CNS. In this study, we examined the expression and the role of Ndrg2 after cortical stab injury. We observed that Ndrg2 expression was elevated in astrocytes surrounding the wounded area as early as day 1 after injury in wild-type mice. Deletion of Ndrg2 resulted in lower induction of reactive astroglial and microglial markers in the injured cortex. Histological analysis showed reduced levels of hypertrophic changes in astrocytes, accumulation of microglia, and neuronal death in Ndrg2-/- mice after injury. Furthermore, activation of the IL-6/signal transducer and activator of transcription 3 (STAT3) pathway, including the expression of IL-6 family cytokines and phosphorylation of STAT3, was markedly reduced in Ndrg2-/- mice after injury. In a culture system, both of Il6 and Gfap were up-regulated in wild-type astrocytes treated with forskolin. Deletion of Ndrg2 attenuated induction of these genes, but did not alter proliferation or migration of astrocytes. Adenovirus-mediated reexpression of Ndrg2 rescued the reduction of IL-6 expression after forskolin stimulation. These findings suggest that Ndrg2 plays a key role in reactive astrogliosis after cortical stab injury through a mechanism involving the positive regulation of IL-6/STAT3 signaling. © 2014 International Society for Neurochemistry.
続きを見る
|
||||||||||||||||||
| 84.
論文 |
論文
|
Yokogawa, Noriaki ; Murakami, Hideki ; Demura, Satoru ; Kato, Satoshi ; Yoshioka, Katsuhito ; Hayashi, Hiroyuki ; Ishii, Takayoshi ; Fujii, Moriyuki ; Igarashi, Takashi ; Tsuchiya, Hiroyuki
概要:
Background: In total en bloc spondylectomy (TES) of upper thoracic spine including the second thoracic (T2) vertebra, T2 nerve roots are usually transected. In this study, we examined the association between transection of
…
the T2 nerve roots and upper-extremity motor function in patients with upper thoracic TES. Copyright:Methods: We assessed 16 patients who underwent upper thoracic TES with bilateral transection of the T2 nerve roots. Patients were divided into three groups: 3 patients without any processing of T1 and upper nerve roots (T2 group), 7 with extensive dissection of T1 nerve roots (T1-2 group), and 6 with extensive dissection of T1 and upper nerve roots (C-T2 group). Postoperative upper-extremity motor function was compared between the groups.Results: Postoperative deterioration of upper-extremity motor function was observed in 9 of the 16 patients (56.3%). Three of the 7 patients in the T1-2 group and all 6 patients in the C-T2 group showed deterioration of upper-extremity motor function, but there was no deterioration in the T2 group. In the T1-2 group, 3 patients showed mild deterioration that did not affect their activities of daily living and they achieved complete recovery at the latest follow-up examination. In contrast, severe dysfunction occurred frequently in the C-T2 group, without recovery at the latest follow-up.Conclusions: The transection of the T2 nerve roots alone did not result in upper-extremity motor dysfunction; rather, the dysfunction is caused by the extensive dissection of the T1 and upper nerve roots. Therefore, transection of the T2 nerve roots in upper thoracic TES seems to be an acceptable procedure with satisfactory outcomes.
続きを見る
|
||||||||||||||||||
| 85.
論文 |
論文
|
Espinoza, J. Luis ; Takami, Akiyoshi ; Trung, Ly Q. ; Nakao, Shinji
概要:
医薬保健研究域医学系<br />The powerful activating receptor NKG2D is expressed by natural killer (NK) cells and promotes cytotoxic
…
lysis of cancer cells expressing NKG2D ligands (NKG2D-Ls). We report the effective induction of NKG2D-Ls, achieved with the naturally occurring polyphenol resveratrol, in a broad range of leukemia cells. In this study, resveratrol upregulated the NKG2D-Ls MHC class I chain-related proteins MICA and MICB, and UL16-binding proteins ULBP1, ULBP2, and ULBP3 in most of the leukemia cells analyzed. Ligand upregulation induced by resveratrol was impaired by pharmacological and genetic disruption of ataxia-telangiectasia mutated kinase, the main regulator of NKG2D-L expression. Leukemia cells treated with resveratrol were more susceptible to killing by NK cells than untreated cells, and the enhanced cytotoxicity of NK cells was blocked by treatment of NK cells with anti-NKG2D mAbs. Interestingly, resveratrol consistently upregulated the NKG2D receptor expression and enhanced NKG2D-mediated functions in resting NK cells obtained from healthy individuals. Therefore, resveratrol has attractive immunotherapeutic potential. © 2013 Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 86.
論文 |
論文
|
Rahman, M. Azizur ; Hasegawa, Hiroshi ; Rahman, M. Mamunur ; Maki, Teruya ; Lim, Richard P.
概要:
Being predominant inorganic arsenicals, methylarsenicals also occur in anaerobic paddy soils. Therefore, this study inve
…
stigated the influence of Fe2+ concentrations and arsenic speciation [arsenate (As(V)) and dimethylarsinate (DMA)] in paddy soils on arsenic uptake in rice plant. Rice seedlings were grown in soil irrigated with a Murashige and Skoog (MS) growth solution containing As(V) or DMA with or without 1.8 mM Fe2+ in excess to the background concentration of total iron (0.03 mM) in the soil. Arsenic concentration in rice roots increased initially and then decreased gradually when the seedlings were grown with excess Fe2+ and As(V). In contrast, arsenic concentration in the roots increased steadily (P < 0.01) when the seedlings were grown without excess Fe2+ and As(V). When the form of the arsenic was DMA, total arsenic (tAs) concentration in rice roots increased gradually (P < 0.01) and was not affected by the addition of excess Fe2+ in the soil. When rice seedlings were grown with As(V), tAs concentration in rice roots and shoots increased steadily (P < 0.01) for gradual increase of Fe2+ concentrations in soil. However, tAs concentration in roots and shoots was independent of Fe2+ concentrations in soil when the form of arsenic was DMA. The tAs concentrations in rice shoots also increased significantly (P < 0.01) with increasing exposure time for both As(V) and DMA. Thus, Fe2+ concentrations in soil affect arsenic uptake in rice plant depending on the speciation of arsenic. © 2013 Springer Science+Business Media Dordrecht.
続きを見る
|
||||||||||||||||||
| 87.
論文 |
論文
|
Li, Zichen ; Takino, Takahisa ; Endo, Yoshio ; Sato, Hiroshi
概要:
An artificial receptor for proMMP-9 was created by fusing tissue inhibitor of MMP-1 (TIMP-1) with type II transmembrane
…
mosaic serine protease (MSP-T1). Expression of MSP-T1 in 293T cells induced binding of proMMP-9, which was processed by MMP-2 activated by membrane type 1 MMP (MT1-MMP). HT1080 cells transfected with the MSP-T1 gene produced activated MMP-9 in collagen gel, and addition of proMMP-2 to the culture augmented it, which resulted in intensive collagen digestion. These cells metastasized into chick embryonic liver more than control cells. Treatment of HT1080 cells with concanavalin A in the presence of exogenous proMMP-2 induced activation of not only proMMP-2 but also proMMP-9. Knockdown of MT1-MMP or TIMP-2 expression with siRNA suppressed activation of both proMMP-2 and proMMP-9. Transfection of TIMP-1 siRNA suppressed cell binding and activation of proMMP-9, but not proMMP-2 activation. Knockdown of a disintegrin and metalloproteinase 10 (ADAM10) expression reduced cell binding and processing of proMMP-9. These results suggest that proMMP-9, which binds to a receptor complex containing TIMP-1 and ADAM10, is activated by the MT1-MMP/MMP-2 axis, and MMP-9 thus activated stimulates cellular proteolysis and metastasis. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 88.
論文 |
論文
|
Cui, Hong ; Okamoto, Yasuo ; Yoshioka, Kazuaki ; Du, Wa ; Takuwa, Noriko ; Zhang, Wei ; Asano, Masahide ; Shibamoto, Toshishige ; Takuwa, Yoh
概要:
Background: Sphingosine-1-phosphate receptor 2 (S1P2) is expressed in vascular endothelial cells (ECs). However, the rol
…
e of S1P2 in vascular barrier integrity and anaphylaxis is not well understood. Endothelial nitric oxide synthase (eNOS) generates nitric oxide to mediate vascular leakage, compromising survival in patients with anaphylaxis. We recently observed that endothelial S1P2 inhibits Akt, an activating kinase of eNOS. Objective: We tested the hypothesis that endothelial S1P2 might suppress eNOS, exerting a protective effect against endothelial barrier disruption and anaphylaxis. Methods: Mice deficient in S1P2 and eNOS underwent antigen challenge or platelet-activating factor (PAF) injection. Analyses were performed to examine vascular permeability and the underlying mechanisms. Results: S1pr2 deletion augmented vascular leakage and lethality after either antigen challenge or PAF injection. PAF injection induced activation of Akt and eNOS in the aortas and lungs of S1pr2-null mice, which were augmented compared with values seen in wild-type mice. Consistently, PAF-induced increase in cyclic guanosine monophosphate levels in the aorta was enhanced in S1pr-null mice. Genetic Nos3 deletion or pharmacologic eNOS blockade protected S1pr2-null mice from aggravation of barrier disruption after antigen challenge and PAF injection. ECs isolated from S1pr2-null mice exhibited greater stimulation of Akt and eNOS, with enhanced nitric oxide production in response to sphingosine-1-phosphate or PAF, compared with that seen in wild-type ECs. Moreover, S1pr2-deficient ECs showed more severe disassembly of adherens junctions with augmented S-nitrosylation of β-catenin in response to PAF, which was restored by pharmacologic eNOS blockade. Conclusion: S1P2 diminishes harmful robust eNOS stimulation and thereby attenuates vascular barrier disruption, suggesting potential usefulness of S1P2 agonists as novel therapeutic agents for anaphylaxis. © 2013 American Academy of Allergy, Asthma & Immunology.
続きを見る
|
||||||||||||||||||
| 89.
論文 |
論文
|
Oyama, Takeru ; Okamoto, Koichi ; Nakamura, Ritsuko ; Tajiri, Ryosuke ; Ikeda, Hiroko ; Ninomiya, Itasu ; Ooi, Akishi
概要:
EGFR and ERBB2 belong to the EGFR gene family. In esophageal squamous cell carcinomas (SCCs), amplification of EGFR or E
…
RBB2 is usually mutually exclusive. EGFR amplification occurs in approximately 15% of SCCs, ERBB2 occurs in less than 5%. Here, we report the co-amplification of EGFR and ERBB2 in an ulcerative and infiltrating-type SCC that measured approximately 4.2 × 2.7 × 1.2cm with a superficial lesion occurring in the thoracic esophagus of a 72-year-old man. Multiplex ligation-dependent probe amplification using representative tumor sections showed gain of CCND1 and coincident amplification of ERBB2 or EGFR or neither. Immunohistochemistry and fluorescence in situ hybridization revealed that the tumor comprised three cancer-cell populations: well-differentiated SCC with high-level ERBB2 amplification and ERBB2 overexpression, more infiltrative poorly-differentiated SCC with high-level EGFR amplification and EGFR overexpression, and poorly-differentiated SCC lacking any ERBB2 or EGFR abnormality. These three populations each had low-level CCND1 amplification and nuclear cyclin D1 overexpression. This histological topology and gene amplification combinations suggested that genetic instability first produced CCND1 amplification, and then ERBB2 or EGFR gene amplification occurred. It is further speculated that during cancer progression and clonal selection indecisive predominance of either clone caused the rare co-amplification of ERBB2 and EGFR in a single chimeric tumor. © 2015 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.<br />発行後1年より全文公開
続きを見る
|
||||||||||||||||||
| 90.
論文 |
論文
|
Cui, Hong ; Okamoto, Yasuo ; Yoshioka, Kazuaki ; Du, Wa ; Takuwa, Noriko ; Zhang, Wei ; Asano, Masahide ; Shibamoto, Toshishige ; Takuwa, Yoh
概要:
Background Sphingosine-1-phosphate receptor 2 (S1P2) is expressed in vascular endothelial cells (ECs). However, the role
…
of S1P 2 in vascular barrier integrity and anaphylaxis is not well understood. Endothelial nitric oxide synthase (eNOS) generates nitric oxide to mediate vascular leakage, compromising survival in patients with anaphylaxis. We recently observed that endothelial S1P2 inhibits Akt, an activating kinase of eNOS. Objective We tested the hypothesis that endothelial S1P 2 might suppress eNOS, exerting a protective effect against endothelial barrier disruption and anaphylaxis. Methods Mice deficient in S1P2 and eNOS underwent antigen challenge or platelet-activating factor (PAF) injection. Analyses were performed to examine vascular permeability and the underlying mechanisms. Results S1pr2 deletion augmented vascular leakage and lethality after either antigen challenge or PAF injection. PAF injection induced activation of Akt and eNOS in the aortas and lungs of S1pr2-null mice, which were augmented compared with values seen in wild-type mice. Consistently, PAF-induced increase in cyclic guanosine monophosphate levels in the aorta was enhanced in S1pr-null mice. Genetic Nos3 deletion or pharmacologic eNOS blockade protected S1pr2-null mice from aggravation of barrier disruption after antigen challenge and PAF injection. ECs isolated from S1pr2-null mice exhibited greater stimulation of Akt and eNOS, with enhanced nitric oxide production in response to sphingosine-1-phosphate or PAF, compared with that seen in wild-type ECs. Moreover, S1pr2-deficient ECs showed more severe disassembly of adherens junctions with augmented S-nitrosylation of β-catenin in response to PAF, which was restored by pharmacologic eNOS blockade. Conclusion S1P2 diminishes harmful robust eNOS stimulation and thereby attenuates vascular barrier disruption, suggesting potential usefulness of S1P2 agonists as novel therapeutic agents for anaphylaxis. © 2013 American Academy of Allergy, Asthma & Immunology.
続きを見る
|
||||||||||||||||||
| 91.
論文 |
論文
|
Oshima, Hiroko ; Popivanova, Boryana K. ; Oguma, Keisuke ; Kong, Dan ; Ishikawa, Tomoo ; Oshima, Masanobu
概要:
金沢大学がん進展制御研究所<br />Cyclooxygenase-2 (COX-2) plays an important role in tumorigenesis through prostaglandin E2 (PGE2) bio
…
synthesis. It has been shown by in vitro studies that PGE2 signaling transactivates epidermal growth factor receptor (EGFR) through an intracellular mechanism. However, the mechanisms underlying PGE2-induced EGFR activation in in vivo tumors are still not fully understood. We previously constructed transgenic mice that develop gastric tumors caused by oncogenic activation and PGE2 pathway induction. Importantly, expression of EGFR ligands, epiregulin, amphiregulin, heparin-binding EGF-like growth factor, and betacellulin, as well as a disintegrin and metalloproteinases (ADAMs), ADAM8, ADAM9, ADAM10, and ADAM17 were significantly increased in the mouse gastric tumors in a PGE2 pathway-dependent manner. These ADAMs can activate EGFR by ectodomain shedding of EGFR ligands. Notably, the extensive induction of EGFR ligands and ADAMs was suppressed by inhibition of the PGE2 receptor EP4. Moreover, EP4 signaling induced expression of amphiregulin and epiregulin in activated macrophages, whereas EP4 pathway was required for basal expression of epiregulin in gastric epithelial cells. In contrast, ADAMs were not induced directly by PGE2 in these cells, suggesting indirect mechanism possibly through PGE2-associated inflammatory responses. These results suggest that PGE2 signaling through EP4 activates EGFR in gastric tumors through global induction of EGFR ligands and ADAMs in several cell types either by direct or indirect mechanism. Importantly, gastric tumorigenesis of the transgenic mice was significantly suppressed by combination treatment with EGFR and COX-2 inhibitors. Therefore, it is possible that inhibition of both COX-2/PGE2 and EGFR pathways represents an effective strategy for preventing gastric cancer. © 2011 Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 92.
論文 |
論文
|
Oshima, Hiroko ; Hioki, Kyoji ; Popivanova, Boryana K. ; Oguma, Keisuke ; Rooijen, Nico van ; Ishikawa, Tomoo ; Oshima, Masanobu
概要:
金沢大学がん研究所<br />Background & Aims Helicobacter pylori infection induces an inflammatory response, which can contribute to
…
gastric tumorigenesis. Induction of cyclooxygenase-2 (COX-2) results in production of prostaglandin E2 (PGE2), which mediates inflammation. We investigated the roles of bacterial infection and PGE2 signaling in gastric tumorigenesis in mice. Methods We generated a germfree (GF) colony of K19-Wnt1/C2mE mice (Gan mice); these mice develop gastric cancer. We examined tumor phenotypes, expression of cytokines and chemokines, and recruitment of macrophages. We also investigated PGE2 signaling through the PGE2 receptor subtype 4 (EP4) in Gan mice given specific inhibitors. Results Gan mice raised in a specific pathogen-free facility developed large gastric tumors, whereas gastric tumorigenesis was significantly suppressed in GF-Gan mice; reconstitution of commensal flora or infection with Helicobacter felis induced gastric tumor development in these mice. Macrophage infiltration was significantly suppressed in the stomachs of GF-Gan mice. Gan mice given an EP4 inhibitor had decreased expression of cytokines and chemokines. PGE2 signaling and bacterial infection or stimulation with lipopolysaccharide induced expression of the chemokine C-C motif ligand 2 (CCL2) (which attracts macrophage) in tumor stromal cells or cultured macrophages, respectively. CCL2 inhibition suppressed macrophage infiltration in tumors, and depletion of macrophages from the tumors of Gan mice led to signs of tumor regression. Wnt signaling was suppressed in the tumors of GF-Gan and Gan mice given injections of tumor necrosis factor-α neutralizing antibody. Conclusions Bacterial infection and PGE2 signaling are required for gastric tumorigenesis in mice; they cooperate to up-regulate CCL2, which recruits macrophage to gastric tumors. Macrophage-derived tumor necrosis factor-α promotes Wnt signaling in epithelial cells, which contributes to gastric tumorigenesis. © 2011 AGA Institute.
続きを見る
|
||||||||||||||||||
| 93.
論文 |
論文
|
Li, Qi ; Wang, Wei ; Machino, Yusuke ; Yamada, Tadaaki ; Kita, Kenji ; Oshima, Masanobu ; Sekido, Yoshitaka ; Tsuchiya, Mami ; Suzuki, Yui ; Nan-ya, Ken-ichiro ; Iida, Shigeru ; Nakamura, Kazuyasu ; Iwakiri, Shotaro ; Itoi, Kazumi ; Yano, Seiji
概要:
Malignant pleural mesothelioma (MPM) is a rare and highly aggressive neoplasm that arises from the pleural, pericardial,
…
or peritoneal lining. Although surgery, chemotherapy, radiotherapy, and combinations of these therapies are used to treat MPM, the median survival of such patients is dismal. Therefore, there is a compelling need to develop novel therapeutics with different modes of action. Ganglioside GM2 is a glycolipid that has been shown to be overexpressed in various types of cancer. However, there are no published reports regarding the use of GM2 as a potential therapeutic target in cases of MPM. In this study, we evaluated the efficacy of the anti-GM2 antibody BIW-8962 as an anti-MPM therapeutic using in vitro and in vivo assays. Consequently, the GM2 expression in the MPM cell lines was confirmed using flow cytometry. In addition, eight of 11 cell lines were GM2-positive (73%), although the GM2 expression was variable. BIW-8962 showed a significant antibody-dependent cellular cytotoxicity activity against the GM2-expressing MPM cell line MSTO-211H, the effect of which depended on the antibody concentration and effector/target ratio. In an in vivo orthotropic mouse model using MSTO-211H cells, BIW-8962 significantly decreased the incidence and size of tumors. Additionally, the GM2 expression was confirmed in the MPM clinical specimens. Fifty-eight percent of the MPM tumors were positive for GM2, with individual variation in the intensity and frequency of staining. These data suggest that anti-GM2 antibodies may become a therapeutic option for MPM patients. In this study, the anti-GM2 antibody BIW-8962 first showed a significant ADCC activity against malignant pleural mesothelioma (MPM) cell line and therapeutic activity in an in vivo orthotropic mouse model using the cell line. Additionally, the GM2 expression was confirmed in the MPM clinical specimens. These data suggest that anti-GM2 antibodies may become a therapeutic option for MPM patients. © 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 94.
論文 |
論文
|
Nakamura, Ritsuko ; Oyama, Takeru ; Tajiri, Ryosuke ; Mizokami, Atsushi ; Namiki, Mikio ; Nakamoto, Masaru ; Ooi, Akishi
概要:
Neural epidermal growth factor-like like (NELL) 1 and 2 constitute a family of multimeric and multimodular extracellular
…
glycoproteins. Although the osteogenic effects of NELL1 and functions of NELL2 in neural development have been reported, their expression and functions in cancer are largely unknown. In this study, we examined expression of NELL1 and NELL2 in renal cell carcinoma (RCC) using clinical specimens and cell lines. We show that, whereas NELL1 and NELL2 proteins are strongly expressed in renal tubules in non-cancerous areas of RCC specimens, their expression is significantly downregulated in cancerous areas. Silencing of NELL1 and NELL2 mRNA expression was also detected in RCC cell lines. Analysis of NELL1/2 promoter methylation status indicated that the CpG islands in the NELL1 and NELL2 genes are hypermethylated in RCC cell lines. NELL1 and NELL2 bind to RCC cells, suggesting that these cells express a receptor for NELL1 and NELL2 that can transduce signals. Furthermore, we found that both NELL1 and NELL2 inhibit RCC cell migration, and NELL1 further inhibits RCC cell adhesion. These results suggest that silencing of NELL gene expression by promoter hypermethylation plays roles in RCC progression by affecting cancer cell behavior. We found that the down-regulation of NELL1 and NELL2 in renal cell carcinoma (RCC) is in part due to the hypermethylation of CpG islands in their putative promoter regions. Furthermore, we found that NELL1 suppresses and NELL2 partially suppresses RCC cell migration, and NELL1 further inhibits RCC cell adhesion. © 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 95.
論文 |
論文
|
金澤, 裕樹 ; 宮地, 利明 ; 八木, 絢子 ; 吉田, 彩 ; 佐藤, 修 ; Kanazawa, Yuki ; Miyati, Toshiaki ; Yagi, Ayako ; Yoshida, Aya ; Sato, Osamu
概要:
The purpose of our study is to develop and to assess a method to calculate T1 and T2 relaxation times using fast spin-echo (FSE) sequences with two echoes per excitation. On a 1.5-T MRI, dual-echo FSE sequences with different repetition
…
times (TR:TR2=2TR1, i.e., TR1=400ms and TR2=800ms) were performed in MnCl2 phantom. Then, we assessed effects of the echo train length (ETL) , effective echo time (TE) , and T2 correction for T1 measurement, when changed each imaging parameter. Besides, we evaluated with comparison between the dual-echo FSE method and mixed sequence. With optimizing FSE imaging parameters, i.e., effective TE, TR, and low ETL, the measurement values of T1 and T2 revealed significantly higher correlation between the dual FSE method and mixed sequence. Moreover, the T2 correction to calculate T1 value could be omitted by shortening first effective TE. The dual-echo FSE method makes it possible to measure T1 and T2 fast and easily and to obtain more detailed information of organizations in human.
続きを見る
|
||||||||||||||||||
| 96.
論文 |
論文
|
Kitoh, Yuya ; Hiramatsu, Yoshihiro
概要:
nSAR analysis using ALOS-2/PALSAR-2 data was conducted to detect landslides around Hakusan volcano, where we observed la
…
ndslide movements in three areas. In the Jinnosukedani area, we detected landslide movement with maximum annual displacement of 200 mm, which is consistent with that of GNSS observations on the ground surface. In the Yunotani area, we detected landslide movement with maximum annual displacement of 200 mm. The landslide area detected by the InSAR analysis is almost coincident with a previous study of InSAR analysis, although the remarkable landslide area with annual displacement of 100–200 mm is narrower than that reported by the previous study. In the Sennindani area, a large slope collapse can be captured by InSAR analysis as a low coherence area. InSAR analysis around Hakusan volcano provides results that are consistent with those of the ground-based monitoring in the field, indicating that InSAR analysis is a useful landslide-monitoring tool.
続きを見る
|
||||||||||||||||||
| 97.
論文 |
論文
|
Matsumoto, Koichi ; Numazawa, Takenori ; Ura, Yutaro ; Ujiyama, T. ; Abe, Susumu ; 松本, 宏一
概要:
金沢大学理工研究域数物科学系<br />Magnetic materials play a significant role in improvement of regenerative cryocooler performance, be
…
cause they have high volumetric specific heat at magnetic transition temperatures. Gadolinium oxysulfide (Gd2O2S, GOS) that has an antiferromagnetic transition at 5 K improved the cooling performance of cryocoolers when it was used in colder side of the second stage regenerator operating below 10 K. Small magnetic susceptibility and specific heat insensitive to magnetic field is important in order to reduce influence of magnetic field on the performance of cryocooler. We measured magnetization and specific heat of ceramic GOS in magnetic field up to 5 T. The magnetization of GOS represented typical temperature dependence for antiferromagnetic materials and no metamagnetic transition was observed. As for specific heat of GOS, peak temperature decreased from 5.5 to 5.0 K with increasing magnetic field from 0 to 5 T and the transitions remained sharp in magnetic fields. Thermal conductivity of GOS was observed to have very small magnetic field dependence. © Published under licence by IOP Publishing Ltd.
続きを見る
|
||||||||||||||||||
| 98.
論文 |
論文
|
Hosoda, Tomonori ; Yamada, Toshiro
概要:
Effect of titanium dioxide (TiO2) on morphology and mechanical properties of poly(vinylidene fluoride) (PVDF)/poly(methy
…
l methacrylate) (PMMA) blend films prepared at different TiO2 contents by a melt casting process was studied. The results showed that tensile moduli in both the machine direction (MD) and the transverse direction (TD) increased with increasing TiO2 content, and calculated tensile moduli based on the Halpin-Tsai and the Kerner model were consistent with experimental ones in both the MD and TD of films containing 10 wt % TiO2. However, experimental tensile moduli exhibited smaller values compared with calculated ones, as the TiO2 content increased to 30 wt %, and it was assumed that this is due to the decrease of crystallinity of PVDF. Morphological observations indicated that TiO2 particles did not affect crystal structures of PVDF and the morphology of PVDF/PMMA amorphous phase, but hindered the crystallization of PVDF. The MD and TD elongation at break exhibited >200 and <20%, respectively. The SEM micrographs revealed that spherulites could deform along the MD when the tensile force was applied along the direction. By contrast, spherulites could not deform along the TD and fractured at very small elongation, owing to the anisotropic morphology of spherulites. © 2014 Wiley Periodicals, Inc.
続きを見る
|
||||||||||||||||||
| 99.
論文 |
論文
|
Fukami, Tatsuki ; Nakajima, Miki ; Matsumoto, Isao ; Zen, Yoh ; Oda, Makoto ; Yokoi, Tsuyoshi
概要:
Human CYP2A13, which is expressed in the respiratory tract, is the most efficient enzyme for the metabolic activation of
…
tobacco-specific nitrosamines such as 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). The relevance of CYP2A13 in carcinogenicity and toxicity in the respiratory tract has been suggested, but the expression of CYP2A13 protein in lung cancer tissues remains to be determined. We first prepared a mouse monoclonal antibody against human CYP2A13. The antibody showed no cross reactivity with the other CYP isoforms including CYP2A6. Using the specific antibody, we performed immunohistochemical analysis for human lung carcinomas. In adenocarcinomas (n=15), all specimens were positive for the staining and five samples showed strong staining. In squamous cell carcinomas (n=15) and large cell carcinomas (n=15), each 14 samples were positive for the staining and two and three samples showed strong staining, respectively. In small cell carcinoma samples (n=15), eight samples were negative for the staining and five samples showed weak or moderate staining. In conclusion, we first found that the expression of CYP2A13 was markedly increased in non-small cell lung carcinomas. The high expression might be associated with the tumor development and progression in non-small cell lung carcinomas. © 2010 Japanese Cancer Association.
続きを見る
|
||||||||||||||||||
| 100.
論文 |
論文
|
Sato, Masako ; Suzuki, Yoshiaki ; Yamada, Fumio ; Somei, Masanori
概要:
Various derivatives of (6R (*),6aR (*))-6-chloro-6a-hydroxy-5, 6,6a,12-tetrahydroindolo[2,3-a]carbazole-5-one (8) and 6-
…
cyano-5- hydroxyindolo[2,3-a]-carbazole (9) are prepared. Preparations of (6R (*),6aR (*),11aR (*))-6-chloro-11a-cyano-6a-hydroxy- (11) and 12-substituted 6-(Z)-aminomethylidene-5,6,6a, 11,11a,12-hexahydroindolo[2,3-a] carbazole-5-ones (15) are also reported. © 2010 The Japan Institute of Heterocyclic Chemistry Received.
続きを見る
|
