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| 1.
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論文
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Takakura, Hisashi ; Ojino, Minoru ; Jue, Thomas ; Yamada, Tatsuya ; Furuichi, Yasuro ; Hashimoto, Takeshi ; Iwase, Satoshi ; Masuda, Kazumi
概要:
Under acute hypoxic conditions, the muscle oxygen uptake (m (Formula presented.) O2) during exercise is reduced by the r
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estriction in oxygen-supplied volume to the mitochondria within the peripheral tissue. This suggests the existence of a factor restricting the m (Formula presented.) O2 under hypoxic conditions at the peripheral tissue level. Therefore, this study set out to test the hypothesis that the restriction in m (Formula presented.) O2 is regulated by the net decrease in intracellular oxygen tension equilibrated with myoglobin oxygen saturation (∆PmbO2) during muscle contraction under hypoxic conditions. The hindlimb of male Wistar rats (8 weeks old, n = 5) was perfused with hemoglobin-free Krebs–Henseleit buffer equilibrated with three different fractions of O2 gas: 95.0%O2, 71.3%O2, and 47.5%O2. The deoxygenated myoglobin (Mb) kinetics during muscle contraction were measured under each oxygen condition with a near-infrared spectroscopy. The ∆[deoxy-Mb] kinetics were converted to oxygen saturation of myoglobin (SmbO2), and the PmbO2 was then calculated based on the SmbO2 and the O2 dissociation curve of the Mb. The SmbO2 and PmbO2 at rest decreased with the decrease in O2 supply, and the muscle contraction caused a further decrease in SmbO2 and PmbO2 under all O2 conditions. The net increase in m (Formula presented.) O2 from the muscle contraction (∆m (Formula presented.) O2) gradually decreased as the ∆PmbO2 decreased during muscle contraction. The results of this study suggest that ΔPmbO2 is a key determinant of the Δm (Formula presented.) O2. © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
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| 2.
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論文
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Ebisu, Haruka ; Iwai-Takekoshi, Lena ; Fujita-Jimbo, Eriko ; Momoi, Takashi ; Kawasaki, Hiroshi
概要:
The molecular mechanisms underlying the formation of the thalamus during development have been investigated intensively.
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Although transcription factors distinguishing the thalamic primordium from adjacent brain structures have been uncovered, those involved in patterning inside the thalamus are largely unclear. Here, we show that Foxp2, a member of the forkhead transcription factor family, regulates thalamic patterning during development. We found a graded expression pattern of Foxp2 in the thalamic primordium of the mouse embryo. The expression levels of Foxp2 were high in the posterior region and low in the anterior region of the thalamic primordium. In Foxp2 (R552H) knockin mice, which have a missense loss-of-function mutation in the forkhead domain of Foxp2, thalamic nuclei of the posterior region of the thalamus were shrunken, while those of the intermediate region were expanded. Consistently, Foxp2 (R552H) knockin mice showed changes in thalamocortical projection patterns. Our results uncovered important roles of Foxp2 in thalamic patterning and thalamocortical projections during development. © 2017 Author.<br />Embargo Period 12 months
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| 3.
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論文
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Yamada, Fumio ; Goto, Aya ; Hasegawa, Masakazu ; Kobayashi, Kensuke ; Somei, Masanori ; 山田, 文夫 ; 染井, 正徳
概要:
金沢大学医薬保健研究域薬学系<br />Various nucleophiles, such as indole, 1,2,3-trimethoxybenzene, anisole, phenol, and pyrrole, reacted
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with 1-hydroxy-Nb-trifluoroacetyltryptamine under the presence of mesyl chloride to give novel series of (3a,8acis)- 1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indoles having a substituent at the 3aposition. Their structures and by-products were strictly determined. © 2017 The Japan Institute of Heterocyclic Chemistry.<br />Embargo Period 12 months
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| 4.
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図書
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translated and annotated by Greg Bailey
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| 5.
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Oki, Shinya ; Sone, Kenbun ; Oda, Katsutoshi ; Hamamoto, Ryuji ; Ikemura, Masako ; Maeda, Daichi ; Takeuchi, Makoto ; Tanikawa, Michihiro ; Mori-Uchino, Mayuyo ; Nagasaka, Kazunori ; Miyasaka, Aki ; Kashiyama, Tomoko ; Ikeda, Yuji ; Arimoto, Takahide ; Kuramoto, Hiroyuki ; Wada-Hiraike, Osamu ; Kawana, Kei ; Fukayama, Masashi ; Osuga, Yutaka ; Fujii, Tomoyuki ; 前田, 大地
概要:
金沢大学医薬保健研究域医学系<br />The histone methyltransferase EZH2, a key epigenetic modifier, is known to be associated with human
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tumorigenesis. However, the physiological importance of EZH2 and its clinical relevance in endometrial cancer remain unclear. Hence, in the present study, we investigated the expression and function of EZH2 in endometrial cancer. In a quantitative real-time PCR analysis of 11 endometrial cancer cell lines and 52 clinical endometrial cancer specimens, EZH2 was significantly overexpressed in cancer cells and tissues compared to that in corresponding normal control cells and tissues. Kaplan-Meier survival analysis using data of the TCGA RNA-seq database and tissue microarrays (TMAs) indicated that EZH2 overexpression is associated with endometrial cancer prognosis. In addition, knockdown of EZH2 using specific siRNAs resulted in growth suppression and apoptosis induction of endometrial cancer cells, accompanied by attenuation of H3K27 trimethylation. Consistent with these results, treatment with GSK126, a specific EZH2 inhibitor, suppressed endometrial cancer cell growth and decreased the number of cancer cell colonies. Furthermore, GSK126 showed additive effects with doxorubicin or cisplatin, which are conventional drugs for treatment of endometrial cancer. Further studies should explore the therapeutic potential of inhibiting EZH2 in patients with endometrial cancer. © Oki et al.
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| 6.
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Li, Zichen ; Takino, Takahisa ; Endo, Yoshio ; Sato, Hiroshi
概要:
An artificial receptor for proMMP-9 was created by fusing tissue inhibitor of MMP-1 (TIMP-1) with type II transmembrane
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mosaic serine protease (MSP-T1). Expression of MSP-T1 in 293T cells induced binding of proMMP-9, which was processed by MMP-2 activated by membrane type 1 MMP (MT1-MMP). HT1080 cells transfected with the MSP-T1 gene produced activated MMP-9 in collagen gel, and addition of proMMP-2 to the culture augmented it, which resulted in intensive collagen digestion. These cells metastasized into chick embryonic liver more than control cells. Treatment of HT1080 cells with concanavalin A in the presence of exogenous proMMP-2 induced activation of not only proMMP-2 but also proMMP-9. Knockdown of MT1-MMP or TIMP-2 expression with siRNA suppressed activation of both proMMP-2 and proMMP-9. Transfection of TIMP-1 siRNA suppressed cell binding and activation of proMMP-9, but not proMMP-2 activation. Knockdown of a disintegrin and metalloproteinase 10 (ADAM10) expression reduced cell binding and processing of proMMP-9. These results suggest that proMMP-9, which binds to a receptor complex containing TIMP-1 and ADAM10, is activated by the MT1-MMP/MMP-2 axis, and MMP-9 thus activated stimulates cellular proteolysis and metastasis. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
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| 7.
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論文
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Kitoh, Yuya ; Hiramatsu, Yoshihiro
概要:
nSAR analysis using ALOS-2/PALSAR-2 data was conducted to detect landslides around Hakusan volcano, where we observed la
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ndslide movements in three areas. In the Jinnosukedani area, we detected landslide movement with maximum annual displacement of 200 mm, which is consistent with that of GNSS observations on the ground surface. In the Yunotani area, we detected landslide movement with maximum annual displacement of 200 mm. The landslide area detected by the InSAR analysis is almost coincident with a previous study of InSAR analysis, although the remarkable landslide area with annual displacement of 100–200 mm is narrower than that reported by the previous study. In the Sennindani area, a large slope collapse can be captured by InSAR analysis as a low coherence area. InSAR analysis around Hakusan volcano provides results that are consistent with those of the ground-based monitoring in the field, indicating that InSAR analysis is a useful landslide-monitoring tool.
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| 8.
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論文
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Matsumoto, Koichi ; Numazawa, Takenori ; Ura, Yutaro ; Ujiyama, T. ; Abe, Susumu ; 松本, 宏一
概要:
金沢大学理工研究域数物科学系<br />Magnetic materials play a significant role in improvement of regenerative cryocooler performance, be
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cause they have high volumetric specific heat at magnetic transition temperatures. Gadolinium oxysulfide (Gd2O2S, GOS) that has an antiferromagnetic transition at 5 K improved the cooling performance of cryocoolers when it was used in colder side of the second stage regenerator operating below 10 K. Small magnetic susceptibility and specific heat insensitive to magnetic field is important in order to reduce influence of magnetic field on the performance of cryocooler. We measured magnetization and specific heat of ceramic GOS in magnetic field up to 5 T. The magnetization of GOS represented typical temperature dependence for antiferromagnetic materials and no metamagnetic transition was observed. As for specific heat of GOS, peak temperature decreased from 5.5 to 5.0 K with increasing magnetic field from 0 to 5 T and the transitions remained sharp in magnetic fields. Thermal conductivity of GOS was observed to have very small magnetic field dependence. © Published under licence by IOP Publishing Ltd.
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| 9.
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論文
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Yamamoto, Sho ; Nagatani, Hirohisa ; Imura, Hisanori ; 永谷, 広久 ; 井村, 久則
概要:
金沢大学理工研究域物質化学系<br />The adsorption and self-aggregation of anionic porphyrins were studied at the polarized water|1,2-di
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chloroethane (DCE) interface by polarization-modulation total internal reflection fluorescence (PM-TIRF) spectroscopy. 5,10,15,20-Tetrakis(4-sulfonatophenyl)porphyrin diacid (H4TPPS2-) and protoporphyrin IX (H2PP2-) exhibited high surface activities at the interface. The selective excitation of interfacial species in PM-TIRF measurements elucidated the potential-induced aggregation mechanism of the porphyrins. The J-aggregates of H4TPPS2- were reversibly formed only at the water|DCE interface by applying appropriate potentials even when the porphyrins exist as monomers in the aqueous and organic solutions. In the H2PP2- system, the slow aggregation process was found in the negative potential region. The spectral characteristics and the signal phase of PM-TIRF indicated that the H2PP2- monomers were adsorbed with relatively standing orientation and that the long axis of the J-aggregates was nearly in plane of the interface. H2PP2- was also investigated at the biomimetic phospholipid-adsorbed water|DCE interface. The competitive adsorption of neutral glycerophospholipids effectively inhibited the potential-dependent adsorption and interfacial aggregation processes of H2PP2-. The results demonstrated that the aggregation state of the charged species can reversibly be controlled at liquid|liquid interfaces as a function of externally applied potential. © 2017 American Chemical Society.<br />Embargo Period 12 months
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| 10.
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論文
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10. Trajectories of Postload Plasma Glucose in the Development of Type 2 Diabetes in Japanese Adults
Oka, Rie ; Shibata, Kyoko ; Sakurai, Masaru ; Kometani, Mitsuhiro ; Yamagishi, Masakazu ; Yoshimura, Kenichi ; Yoneda, Takashi ; 大家, 理恵 ; 柴田, 恭子 ; 櫻井, 勝 ; 米谷, 充弘 ; 山岸, 正和 ; 吉村, 健一 ; 米田, 隆
概要:
金沢大学附属病院研修医・専門医総合教育センター<br />We aimed to clarify how the trajectories of 1-hour postload plasma glucose (PG) and 2-hour
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PG were different in the development of type 2 diabetes. Using data of repeated health checkups in Japanese workers from April 2006 to March 2016, longitudinal changes of fasting, 1-hour, and 2-hour PG on the oral glucose tolerance test were analyzed with a linear mixed effects model. Of the 1464 nondiabetic subjects at baseline, 112 subjects progressed to type 2 diabetes during the observation period (progressors). In progressors, 1-hour PG and 2-hour PG showed gradual increases with slopes of 1.33 ± 0.2 and 0.58 ± 0.2 mg/dL/year, respectively, followed by a steep increase by which they attained diabetes. Until immediately before the diabetes transition, age- and sex-adjusted mean level of 2-hour PG was 149 ± 2.7 mg/dL, 34 ± 2.7 (30%) higher compared to nonprogressors, while that of 1-hour PG was 206 ± 4.1 mg/dL, 60 ± 4.3 mg/dL (41%) higher compared to nonprogressors. In conclusion, diabetes transition was preceded by a mild elevation of 2-hour PG for several years or more. The elevation in 1-hour PG was larger than that of 2-hour PG until immediately before the transition to diabetes. © 2017 Rie Oka et al.<br />Embargo Period 12 months
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