Real-Time Fluorescence Imaging of the DNA Damage Repair Response during Mitosis

フォーマット:

論文

責任表示:

Miwa, Shinji ; Yano, Shuya ; Yamamoto, Mako ; Matsumoto, Yasunori ; Uehara, Fuminari ; Hiroshima, Yukihiko ; Toneri, Makoto ; Murakami, Takashi ; Kimura, Hiroaki ; Hayashi, Katsuhiro ; Yamamoto, Norio ; Efimova, Elena V. ; Tsuchiya, Hiroyuki ; Hoffman, Robert M.

言語:

英語

出版情報:

Wiley-Liss Inc., 2015-04-01

著者名:

Miwa, Shinji

Yano, Shuya

Yamamoto, Mako

Matsumoto, Yasunori

Uehara, Fuminari

Hiroshima, Yukihiko

Toneri, Makoto

Murakami, Takashi

Kimura, Hiroaki

Hayashi, Katsuhiro

Yamamoto, Norio

Efimova, Elena V.

Tsuchiya, Hiroyuki

Hoffman, Robert M.

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掲載情報:

Journal of Cellular Biochemistry

ISSN:

0730-2312      

巻:

116

通号:

4

開始ページ:

661

終了ページ:

666

バージョン:

author

概要:

The response to DNA damage during mitosis was visualized using real-time fluorescence imaging of focus formation by the DNA-damage repair (DDR) response protein 53BP1 linked to green fluorescent protein (GFP) (53BP1-GFP) in the MiaPaCa-2Tet-On pancreatic cancer cell line. To observe 53BP1-GFP foci during mitosis, MiaPaCa-2Tet-On 53BP1-GFP cells were imaged every 30 min by confocal microscopy. Time-lapse imaging demonstrated that 11.4 ± 2.1% of the … mitotic MiaPaCa-2Tet-On 53BP1-GFP cells had increased focus formation over time. Non-mitotic cells did not have an increase in 53BP1-GFP focus formation over time. Some of the mitotic MiaPaCa-2Tet-On 53BP1-GFP cells with focus formation became apoptotic. The results of the present report suggest that DNA strand breaks occur during mitosis and undergo repair, which may cause some of the mitotic cells to enter apoptosis in a phenomenon possibly related to mitotic catastrophe. J. Cell. Biochem. 116: 661-666, 2015. © 2014 Wiley Periodicals, Inc. 続きを見る

URL:

http://hdl.handle.net/2297/43946