Activity of bone morphogenetic protein-7 after treatment at various temperatures: Freezing vs. pasteurization vs. allograft

フォーマット:

論文

責任表示:

Takata, Munetomo ; Sugimoto, Naotoshi ; Yamamoto, Norio ; Shirai, Toshiharu ; Hayashi, Katsuhiro ; Nishida, Hideji ; Tanzawa, Yoshikazu ; Kimura, Hiroaki ; Miwa, Shinji ; Takeuchi, Akihiko ; Tsuchiya, Hiroyuki

言語:

英語

出版情報:

Elsevier, 2011-12-01

著者名:

Takata, Munetomo

Sugimoto, Naotoshi

Yamamoto, Norio

Shirai, Toshiharu

Hayashi, Katsuhiro

Nishida, Hideji

Tanzawa, Yoshikazu

Kimura, Hiroaki

Miwa, Shinji

Takeuchi, Akihiko

Tsuchiya, Hiroyuki

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掲載情報:

Cryobiology

ISSN:

0011-2240      

巻:

63

通号:

3

開始ページ:

235

終了ページ:

239

バージョン:

author

概要:

Insufficient bone union is the occasional complication of biomechanical reconstruction after malignant bone tumor resection using temperature treated tumor bearing bone; freezing, pasteurization, and autoclaving. Since bone morphogenetic protein (BMP) plays an important role in bone formation, we assessed the amount and activity of BMP preserved after several temperature treatments, including -196 and -73 °C for 20 min, 60 and 100 °C for 30 … min, 60 °C for 10 h following -80 °C for 12 h as an allograft model, and 4 °C as the control. The material extracted from the human femoral bone was treated, and the amount of BMP-7 was analyzed using an enzyme-linked immunosorbent assay. Then, the activity of recombinant human BMP-7 after the treatment was assessed using a bioassay with NIH3T3 cells and immunoblotting analysis to measure the amount of phospho-Smad, one of the signaling substrates that reflect the intracellular reaction of BMPs. Both experiments revealed that BMP-7 was significantly better preserved in the hypothermia groups. The percentages of the amount of BMP-7 in which the control group was set at 100% were 114%, 108%, 70%, 49%, and 53% in the -196, -73, 60, 100 °C, and the allograft-model group, respectively. The percentages of the amount of phospho-Smad were 89%, 87%, 24%, 4.9%, and 14% in the -196, -73, 60, 100 °C, and the allograft-model group, respectively. These results suggested that freezing possibly preserves osteoinductive ability than hyperthermia treatment. © 2011 Elsevier Inc. All rights reserved. 続きを見る

URL:

http://hdl.handle.net/2297/29486