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| 1.
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論文
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Bekki, Kanae ; Toriba, Akira ; Ning, Tang ; Kameda, Takayuki ; Hayakawa, Kazuichi ; 戸次, 加奈江 ; 鳥羽, 陽 ; 唐, 寧 ; 亀田, 貴之 ; 早川, 和一
概要:
金沢大学理工研究域自然システム学系<br />Polycyclic aromatic hydrocarbons (PAHs) are included in various environmental pollutants such as
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airborne particles and have been reported to induce a variety of toxic effects. On the other hand, PAH derivatives are generated from PAHs both through chemical reaction in the atmosphere and metabolism in the body.PAH derivatives have become known for their specific toxicities such as estrogenic/antiestrogenic activities and oxidative stress, and correlations between the toxicities and structures of PAH derivatives have been shown in recent studies. These studies are indispensable for demonstrating the health effects of PAH derivatives, since they would contribute to the comprehensive toxicity prediction of many kinds of PAH derivatives.<br />多環芳香族炭化水素類（PAHs）は大気粉塵などの多種類の環境汚染物質に含まれ，長年の研究によって多様な生体影響を引き起こすことが知られている．一方で，PAHsは生体内での代謝反応や，大気中での化学反応によって多種多様な誘導体を生成することが知られている．近年では，PAHだけでなくPAH誘導体の毒性影響が着目されており，エストロゲン様/抗エストロゲン作用，酸化ストレス反応など，PAHとは異なる誘導体独自の毒性影響の存在が報告されている．また，生成するPAH誘導体には多くの構造異性体が存在するが，PAH誘導体が示す毒性作用と構造との間に相関性，いわゆる構造活性相関があることが示されている．以上の研究は，環境中に存在するPAH 誘導体の生体影響を解明する上で重要な研究であるとともに，多種多様なPAH誘導体の総合的な毒性影響予測に貢献できると考えられる．
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| 2.
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Koyama, Kazuo ; Hirao, Takashi ; Toriba, Akira ; Hayakawa, Kazuichi
概要:
The quality of starch-containing foods may be significantly impaired by contamination with very small amounts of α-amyla
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se, which can enzymatically hydrolyze the starch and cause viscosity loss. Thus, for quality control, it is necessary to have an analytical method that can measure low amylase activity. We developed a sensitive analytical method for measuring the activity of α-amylase (from Bacillus subtilis) in starch-containing foods. The method consists of six steps: (1) crude extraction of α-amylase by centrifugation and filtration; (2) α-amylase purification by desalting and anion-exchange chromatography; (3) reaction of the purified amylase with boron-dipyrromethene (BODIPY)-labeled substrate, which releases a fluorescent fragment upon digestion of the substrate, thus avoiding interference from starch derivatives in the sample; (4) stopping the reaction with acetonitrile; (5) reversed-phase solid-phase extraction of the fluorescent substrate to remove contaminating dye and impurities; and (6) separation and measurement of BODIPY fluorescence by HPLC. The proposed method could quantify α-amylase activities as low as 10mU/mL, which is enough to reduce the viscosity of starch-containing foods. © 2012 John Wiley & Sons, Ltd.
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Li, Ying ; Tang, Ning ; Inagaki, Fuyuhiko ; Mukai, Chisato ; Hayakawa, Kazuichi
概要:
1,3-Dialkylimidazolium-based ionic liquids were chemically synthesized and bonded on the surface of magnetic nanoparticl
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es (MNPs) with easy one-step reaction. The obtained six kinds of ionic liquid modified MNPs were characterized with transmission electron microscopy, thermogravimetric analysis, magnetization, and FTIR, which owned the high adsorption capacity due to the nanometer size and high-density modification with ionic liquids. Functionality of MNPs with ionic liquids greatly influenced the solubility of the MNPs with organic solvents depending on the alkyl chain length and the anions of the ionic liquids. Moreover, the obtained MNPs showed the specific extraction efficiency to organic pollutant, polycyclic aromatic hydrocarbons, while superparamagnetic property of the MNPs facilitated the convenient separation of MNPs from the bulks water samples. © 2013 Ying Li et al.
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Yano, Sachiko ; Kitamura, Kei-ichiro ; Satoh, Yusuke ; Nakano, Masaki ; Hattori, Atsuhiko ; Sekiguchi, Toshio ; Ikegame, Mika ; Nakashima, Hiroshi ; Omori, Katsunori ; Hayakawa, Kazuichi ; Chiba, Atsuhiko ; Sasayama, Yuichi ; Ejiri, Sadakazu ; Mikuni-Takagaki, Yuko ; Mishima, Hiroyuki ; Funahashi, Hisayuki ; Sakamoto, Tatsuya ; Suzuki, Nobuo
概要:
Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteo
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blasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-αB ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain. © 2013 Zoological Society of Japan.
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| 5.
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論文
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早川, 和一 ; Hayakawa, Kazuichi
概要:
金沢大学医薬保健研究域薬学系<br />水試料中のPAH,NPAH類を対象にHPLC/蛍光検出法,HPLC/化学発光検出法を用いた超高感度分析法を開発し,前者を用いて日本海及び周辺海域のPAH汚染の現状を明らかにした。また,PAH水酸化体と
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PAHキノン体の酵母two-hybrid法を用いた内分泌かく乱作用とキンギョ鱗培養システムに及ぼす影響に関連があり,作用を示す化合物との間に構造活性相関があることを明らかにした。<br />Analytical methods for PAHs and NPAHs in water were developed by the HPLC/fluorescence detection and HPLC/chemiluminescence detection, respectively. The contamination of PAHs in the Japan Sea and surrounding areas was clarified by using the former method. Endocrine disrupting activities of hydroxy PAHs and quinoid PAHs were evaluated by using the east two-hybrid assay and goldfish-scale incubator assay. The active compounds by the assay systems showed a structure-activity relationship.<br />研究課題/領域番号:21390034, 研究期間(年度):2009 – 2012
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| 6.
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論文
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早川, 和一 ; Hayakawa, Kazuichi
概要:
水試料中のPAH,NPAH類を対象にHPLC/蛍光検出法,HPLC/化学発光検出法を用いた超高感度分析法を開発し,前者を用いて日本海及び周辺海域のPAH汚染の現状を明らかにした。また,PAH水酸化体とPAHキノン体の酵母two-hybrid
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法を用いた内分泌かく乱作用とキンギョ鱗培養システムに及ぼす影響に関連があり,作用を示す化合物との間に構造活性相関があることを明らかにした。<br />Analytical methods for PAHs and NPAHs in water were developed by the HPLC/fluorescence detection and HPLC/chemiluminescence detection, respectively. The contamination of PAHs in the Japan Sea and surrounding areas was clarified by using the former method. Endocrine disrupting activities of hydroxy PAHs and quinoid PAHs were evaluated by using the east two-hybrid assay and goldfish-scale incubator assay. The active compounds by the assay systems showed a structure-activity relationship.<br />研究課題/領域番号:21390034, 研究期間(年度):2009–2012
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