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図書 |
図書
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edited by M. Ida, T. Kamae, M. Kobayashi
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論文 |
論文
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Hasegawa, Hiroshi ; Rahman, M.A. ; Saitou, K. ; Kobayashi, M. ; Okumura, C.
概要:
金沢大学理工研究域物質化学系<br />In this study, the effects of chelating ligands on iron movement in growth medium, iron bioavailabil
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ity, and growth of radish sprouts (Raphanus sativus) were investigated. Iron is an important nutrient for plant growth, yet the insoluble state of iron hydroxides in alkaline conditions decreases its bioavailability. Iron chelates increase iron uptake and have been used in agriculture to correct iron chlorosis. While previous studies have reported the effects of chelating ligands on iron solubility and bioavailability, the present study elucidates the pattern of iron movement by chelating ligands in plant growth medium. The apparent mobility of iron in growth medium was calculated using a '4-box' model. Ethylenediaminedisuccinic acid (EDDS) and hydroxy-iminodisuccinic acid (HIDS) produced the highest apparent mobility of iron from the bottom layer of the medium (initially 10-4M Fe(III)) to the upper layer (no iron), followed by glutamatediacetic acid (GLDA), ethylenediaminetetraacetic acid (EDTA), methylglycinediacetic acid (MGDA), and iminodisuccinic acid (IDS). Iron movement in the growth medium was influenced by the chelating ligand species, pH, and ligand exposure time. The iron uptake and growth of radish sprouts were related to the iron mobility produced by the chelating ligands. These results suggest that, in alkaline media, chelating ligands dissolve the hardly soluble iron hydroxide species, thus increasing iron mobility, iron uptake, and plant growth. HIDS, which is biodegradable, was one of the most effective ligands studied; therefore, this compound would be a good alternative to other environmentally persistent chelating ligands. © 2011 Elsevier B.V.<br />2011年8月より全文公開.
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| 3.
論文 |
論文
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Hasegawa, Hiroshi ; Rahman, M. Azizur ; Saitou, K. ; Kobayashi, M. ; Okumura, Chikako
概要:
In this study, the effects of chelating ligands on iron movement in growth medium, iron bioavailability, and growth of r
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adish sprouts (Raphanus sativus) were investigated. Iron is an important nutrient for plant growth, yet the insoluble state of iron hydroxides in alkaline conditions decreases its bioavailability. Iron chelates increase iron uptake and have been used in agriculture to correct iron chlorosis. While previous studies have reported the effects of chelating ligands on iron solubility and bioavailability, the present study elucidates the pattern of iron movement by chelating ligands in plant growth medium. The apparent mobility of iron in growth medium was calculated using a '4-box' model. Ethylenediaminedisuccinic acid (EDDS) and hydroxy-iminodisuccinic acid (HIDS) produced the highest apparent mobility of iron from the bottom layer of the medium (initially 10-4M Fe(III)) to the upper layer (no iron), followed by glutamatediacetic acid (GLDA), ethylenediaminetetraacetic acid (EDTA), methylglycinediacetic acid (MGDA), and iminodisuccinic acid (IDS). Iron movement in the growth medium was influenced by the chelating ligand species, pH, and ligand exposure time. The iron uptake and growth of radish sprouts were related to the iron mobility produced by the chelating ligands. These results suggest that, in alkaline media, chelating ligands dissolve the hardly soluble iron hydroxide species, thus increasing iron mobility, iron uptake, and plant growth. HIDS, which is biodegradable, was one of the most effective ligands studied; therefore, this compound would be a good alternative to other environmentally persistent chelating ligands. © 2011 Elsevier B.V.
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論文 |
論文
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Ogai, Kazuhiro ; Matsumoto, Masaru ; Minematsu, T. ; Kitamura, K. ; Kobayashi, M. ; Sugama, Junko ; Sanada, H.
概要:
Objective: A novel skin assessment tool named 'skin blotting' has been recently developed, which can easily predict the
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skin status to avoid its deterioration. The aim of this study was to propose a normalization method for skin blotting to compensate for individual differences that can hamper the quantitative comparisons and clinical applications. Methods: To normalize individual differences, we utilized a total protein as a 'normalizer' with calibration curves. For evaluation, we performed a simple simulation experiment, in which the same concentration of a protein of interest [tumour necrosis factor (TNF)-α] was applied at different volumes as a virtual individual difference. Moreover, to demonstrate the applicability of this normalization, male volunteers were recruited for skin blotting followed by the estimation of TNF-α with normalization. Results: We obtained good calibration curves for total protein (R2 = 0.995) and TNF-α (R2 = 0.997), both of which were necessary for an exact quantification. In the simulation experiment, we estimated the exact concentration of TNF-α regardless of the applied volume, demonstrating the applicability of this normalization method in skin blotting. Further, skin blotting on human subjects showed a wide range of variation in the total protein content, although the normalization was thought to reduce such individual variations. Conclusion: This study has proposed total protein normalization for skin blotting with calibration curves. This method may strengthen the quantitative performance of skin blotting, which may expand the applicability of this method as a skin assessment tool in broader fields, such as nursing and cosmetology. Résumé: Objectif: Un nouvel outil d'évaluation de la peau nommé "skin blotting" a été récemment mis au point, qui peut facilement prédire l'état de la peau pour éviter sa détérioration. Le but de cette étude est de proposer une méthode de normalisation pour la "peau buvard" pour compenser les différences individuelles qui peuvent entraver les comparaisons quantitatives et applications cliniques. Méthodes: Pour normaliser les différences individuelles, nous avons utilisé le paramètre protéine totale comme «normalisateur» avec des courbes d'étalonnage. Pour l'évaluation, nous avons réalisé une expérience de simulation simple, dans lequel la même concentration d'une protéine d'intérêt [facteur de nécrose tumorale (TNF) -α] a été appliqué à des volumes différents en tant que différence individuelle virtuel. En outre, pour démontrer l'applicabilité de cette normalisation, les volontaires masculins ont été recrutés pour le skin blotting suivi par l'estimation de TNF-α avec la normalisation. Resultats: Nous avons obtenu de bonnes courbes d'étalonnage pour les protéines totales (R2 = 0.995) et le TNF-α (R2 = 0.997), qui étaient tous deux nécessaires pour une quantification exacte. Dans l'expérience de simulation, nous avons estimé la concentration exacte de TNF-α quel que soit le volume appliqué, ce qui démontre l'applicabilité de cette méthode de normalisation de la "peau buvard". En outre, le skin blotting sur sujets humains a montré une large gamme de variation de la teneur totale en protéines, bien que la normalisation ait dû réduire ces variations individuelles. Conclusion: Cette étude a proposé une normalisation de protéines pour le skin blotting avec des courbes d'étalonnage. Cette méthode peut augmenter la performance quantitative de la technique, ce qui peut élargir l'applicabilité de cette méthode comme un outil d'évaluation de la peau dans des domaines plus larges, comme les soins infirmiers et de la cosmétologie. © 2015 Society of Cosmetic Scientists and the Société Française de Cosmétologie.<br />発行後1年より全文公開
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| 5.
論文 |
論文
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Ogai, Kazuhiro ; Matsumoto, Masaru ; Minematsu, T. ; Kitamura, Keiichiro ; Kobayashi, M. ; Sugama, Junko ; Sanada, Hiromi
概要:
Objective A novel skin assessment tool named 'skin blotting' has been recently developed, which can easily predict the s
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kin status to avoid its deterioration. The aim of this study was to propose a normalization method for skin blotting to compensate for individual differences that can hamper the quantitative comparisons and clinical applications. Methods To normalize individual differences, we utilized a total protein as a 'normalizer' with calibration curves. For evaluation, we performed a simple simulation experiment, in which the same concentration of a protein of interest [tumour necrosis factor (TNF)-α] was applied at different volumes as a virtual individual difference. Moreover, to demonstrate the applicability of this normalization, male volunteers were recruited for skin blotting followed by the estimation of TNF-α with normalization. Results We obtained good calibration curves for total protein (R2 = 0.995) and TNF-α (R2 = 0.997), both of which were necessary for an exact quantification. In the simulation experiment, we estimated the exact concentration of TNF-α regardless of the applied volume, demonstrating the applicability of this normalization method in skin blotting. Further, skin blotting on human subjects showed a wide range of variation in the total protein content, although the normalization was thought to reduce such individual variations. Conclusion This study has proposed total protein normalization for skin blotting with calibration curves. This method may strengthen the quantitative performance of skin blotting, which may expand the applicability of this method as a skin assessment tool in broader fields, such as nursing and cosmetology. © 2015 Society of Cosmetic Scientists and the Société Française de Cosmétologie.<br />Embargo Period 12 months
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論文 |
論文
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Ogai, Kazuhiro ; Matsumoto, M. ; Aoki, M. ; Minematsu, Takeo ; Kitamura, K. ; Kobayashi, M. ; Sanada, H. ; Sugama, Junko
概要:
Objective: A state of chronic inflammation, characterized by an increased level of tumour necrosis factor-alpha (TNF-α),
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is often found in the obese population. The negative effects of elevated TNF-α are not limited to systemic metabolism. It also reportedly affects skin integrity. Recently, the relationship between obesity and skin fragility was reported; however, there has been little insight into how the level of TNF-α in the skin in situ is related to the severity of obesity. In this study, we aimed to measure the level of TNF-α on the skin and to find the relationship between obesity and the level of TNF-α detected on the skin. Methods: We used a novel, non-invasive method called quantitative skin blotting. Fifty-nine healthy (but some were classified as being overweight or obese) Japanese males were enrolled as subjects. The levels of TNF-α detected on the abdominal and thigh skin along with the body composition were measured, followed by a correlation analysis. Results: Significant positive correlations were found between the levels of TNF-α detected on the skin and the severity of obesity such as body mass index (BMI), body fat weight and visceral fat rating. Conclusion: We found that high levels of TNF-α were detected on the skin of Japanese obese males, which implied the higher TNF-α in the skin. The elevation of skin TNF-α may be one factor related to skin fragility that is often found in obese individuals. © 2016 John Wiley & Sons Ltd.<br />Embargo Period 12 months
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