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Nesfatin-1 evokes Ca2+ signaling in isolated vagal afferent neurons via Ca2+ influx through N-type channels
- フォーマット:
- 論文
- 責任表示:
- Iwasaki, Yusaku ; Nakabayashi, Hajime ; Kakei, Masafumi ; Shimizu, Hiroyuki ; Mori, Masatomo ; Yada, Toshihiko
- 言語:
- 英語
- 出版情報:
- Elsevier, 2009-12-18
- 著者名:
Iwasaki, Yusaku Nakabayashi, Hajime Kakei, Masafumi Shimizu, Hiroyuki Mori, Masatomo Yada, Toshihiko - 掲載情報:
- Biochemical and Biophysical Research Communications
- ISSN:
- 0006-291x
- 巻:
- 390
- 通号:
- 3
- 開始ページ:
- 958
- 終了ページ:
- 962
- バージョン:
- author
- 概要:
- 金沢大学医薬保健研究域医学系<br />Nesfatin-1, processed from nucleobindin 2, is an anorexigenic peptide expressed in the brain and several peripheral tissues including the stomach and pancreas. Peripheral, as well as intracerebroventricular, admini … stration of nesfatin-1 suppresses feeding behavior, though underlying mechanisms are unknown. In this study, we examined effects of nesfatin-1 on cytosolic Ca2+ concentration ([Ca2+]i) in the neurons isolated from the vagal afferent nodose ganglion of mice. Nesfatin-1 at 10-10-10-8 M increased [Ca2+]i in the isolated neurons in a concentration-dependent manner, and at 10-8 M it increased [Ca2+]i in 33 out of 263 (12.5%) neurons. These responses were inhibited under Ca2+-free conditions and by N-type Ca2+ channel blocker, ω-conotoxin GVIA. All the nesfatin-1-responsive neurons also exhibited [Ca2+]i responses to capsaicin and cholecystokinin-8. These results provide direct evidence that nesfatin-1 activates vagal afferent neurons by stimulating Ca2+ influx through N-type channels, demonstrating the machinery through which peripheral nesfatin-1 can convey signals to the brain. © 2009 Elsevier Inc. All rights reserved. 続きを見る
- URL:
- http://hdl.handle.net/2297/20509
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Henry Stewart Talks Ltd |
American Society for Clinical Investigation |
Wellness and Health Care Society, ウェルネス・ヘルスケア学会 |
金沢大学環日本海域環境研究センター = Kanazawa University Institute of Nature and Environmental Technology |
American Physical Society |