Type III Cu mutants of Myrothecium verrucaria bilirubin oxidase

フォーマット:

論文

責任表示:

Shimizu, Atsushi ; Samejima, Tatsuya ; Hirota, Shun ; Yamaguchi, Shotaro ; Sakurai, Nobuhiko ; Sakurai, Takeshi

言語:

英語

出版情報:

日本生化学会 = Japanese Biochemical Society, 2003-06-01

著者名:

Shimizu, Atsushi

Samejima, Tatsuya

Hirota, Shun

Yamaguchi, Shotaro

Sakurai, Nobuhiko

Sakurai, Takeshi

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掲載情報:

Journal of Biochemistry

ISSN:

0021-924x      

巻:

133

通号:

6

開始ページ:

767

終了ページ:

772

バージョン:

publisher

概要:

金沢大学理工研究域物質化学系<br />Type III Cu ligand, His456 and His458, of Myrothecium verrucaria (MT-1) bilirubin oxidases (BO) [EC 1.3.3.5] were doubly mutated as to Lys, Asp, and Val. In spite of perturbation of the type III Cu centers, these m … utants were pale blue or colourless when isolated. However, they became intense blue on reaction with reducing agents such as dithionite, ascorbate, hexacyanoferrate(II), and octacyanotangstate(IV) under air, or with an oxidizing agent such as hexacyanoferrate(III), indicating that they are in mixed forms when expressed in Aspergillus oryzae. His456.458Lys and His456.458Asp mutated as to potential coordinating groups showed weak BO and ferroxidase activities, while His 456.458Val mutated as to non-coordinating groups showed no enzyme activity at all. 続きを見る

URL:

http://hdl.handle.net/2297/16743