Activator protein-1 responsive to the group II metabotropic glutamate receptor subtype in association with intracellular calcium in cultured rat cortical neurons

フォーマット:

論文

責任表示:

Sugiyama, Chie ; Nakamichi, Noritaka ; Ogura, Masato ; Honda, Eriko ; Maeda, Sayaka ; Taniura, Hideo ; Yoneda, Yukio

言語:

英語

出版情報:

Elsevier, 2007-12-01

著者名:

Sugiyama, Chie

Nakamichi, Noritaka

Ogura, Masato

Honda, Eriko

Maeda, Sayaka

Taniura, Hideo

Yoneda, Yukio

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掲載情報:

Neurochemistry International

ISSN:

0197-0186      

巻:

51

通号:

8

開始ページ:

467

終了ページ:

475

バージョン:

author

概要:

金沢大学大学院自然科学研究科分子作用学<br />Activation of ionotropic glutamate (Glu) receptors, such as N-methyl-d-aspartate receptors, is shown to modulate the gene transcription mediated by the transcription factor activator protein-1 (AP1) composed o … f Fos and Jun family proteins in the brain, while little attention has been paid to the modulation of AP1 expression by metabotropic Glu receptors (mGluRs). In cultured rat cortical neurons, where constitutive expression was seen with all groups I, II and III mGluR subtypes, a significant and selective increase was seen in the DNA binding activity of AP1 120 min after the brief exposure to the group II mGluR agonist (2S,2′R,3′R)-2-(2′,3′-dicarboxycyclopropyl)glycine (DCG-IV) for 5 min. In cultured rat cortical astrocytes, by contrast, a significant increase was induced by a group I mGluR agonist, but not by either a group II or III mGluR agonist. The increase by DCG-IV was significantly prevented by a group II mGluR antagonist as well as by either an intracellular Ca2+ chelator or a voltage-sensitive Ca2+ channel blocker, but not by an intracellular Ca2+ store inhibitor. Moreover, DCG-IV significantly prevented the increase of cAMP formation by forskolin in cultured neurons. Western blot analysis revealed differential expression profiles of Fos family members in neurons briefly exposed to DCG-IV and NMDA. Prior or simultaneous exposure to DCG-IV led to significant protection against neuronal cell death by NMDA. These results suggest that activation of the group II mGluR subtype would modulate the gene expression mediated by AP1 through increased intracellular Ca2+ levels in cultured rat cortical neurons. © 2007. 続きを見る

URL:

http://hdl.handle.net/2297/7392