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田崎, 和江 ; Tazaki, Kazue
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金沢大学理工研究域地球社会基盤学系<br />Nitrogen both in the recent and fossil shells were analysed by micro-Kjeldahl method, the latter
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being from the Johmon shell-mounds (2000-9000 years B.P.) in the Kanto district, Japan. It is difficult to separate the organic metrials from calcium carbonate without any loss especially in the fossil shells, so the nitrogen analysis of the shell should be carried out with much calcium carbonate, not to remove it. The writer is successful in quantitative nitrogen analysis of the organic materials with much calcium carbonate (Fig. 1). Both the fossil and recent samples analysed are Anadara broughtoni, A. subcrenata, Ostrea gigas, Corbicula iaponica, C. leana, Meretrix lusoria, Mya japonica and Neverita didyma, which are shown in Table 1 with places of occurrence, absolute ages, and weight percents of nitrogen in the shell. In so far as the fossil shells of the Johmon shellmounds are concerned, it is concluded that nitrogen content of the shell indicates rapid diminishing in 2000 years and slow decreasing since then (Fig. 2).
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| 2.
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出石, 隆 ; 鏑木, 光朗 ; 玉鉾, 良三 ; 米谷, 数子 ; 中原, 吉晴 ; 能崎, 克巳 ; 竹内, 昭 ; 亀田, 富子 ; 松扉, 繁麿 ; 小倉, 幸春
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田辺, 啓三 ; 幸山, 彰一 ; 光谷, 音吉 ; 高瀬, 允 ; 竹内, 昭 ; 松扉, 繁麿 ; 野々市, 幸子 ; 亀田, 富子 ; 出石, 隆
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米谷, 数子
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中原, 吉晴
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幸山, 彰ー ; 亀田, 富子
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鏑木, 光朗
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| 9.
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Itagaki, Eiji ; Fujita, Takeshi ; Sato, Ryo ; 板垣, 英治
概要:
A preparation containing formate dehydrogenase, cytochrome b1 and nitrate reductas_??_ was solubilized and partially purified from the particulate fraction of E. coli cells growr_??_ anaerobically under the conditions favorable
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for nitrate respiration. Some properties o_??_ formate dehydrogenase and cytochrome b1 in this purified preparation were investigated It was suggested that formate dehydrogenase is a metalloflavoprotein with essential sulf_??_ hydryl groups. Its activity was strongly bu_??_ reversibly inhibited by molecular oxyge_??_ The prosthetic group of cytochrome b1 was decisively identified as protoheme, and it was found that the cytochrome is autoxidizable. The reduction of cytochrome b1 by formate was shown to require, in addition to formate dehydrogenase, a lipid-soluble factor which could be replaced by vitamin K3 in the solubilized system.We are indefted to Drs. H. Maeno and S. Sakakibara for generous gifts of crude snake venonand crystalline protohemin, respectively.
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| 10.
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Fujita, Takeshi ; Itagaki, Eiji ; Sato, Ryo ; 板垣, 英治
概要:
Cytochrome b1 was solubilized from the particulate fraction of Escherichia coli with the aid of snake venom and deoxycho
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late and was extensively purified by ammonium sul-fate fractionation and hydroxylapatite column chromatography. The purifed preparation was still contaminated by a colorless impurity which sedimented more slowly than the cytochrome in the ultracentrifugal field; the purity being estimated to be 50 to 70 per cent. A molecular weight of 600, 000 to 800, 000 could be expected for the cytochrome from its sedimentation constant. On the other hand, aminimum molecular weight of about 160, 000 was obtained from the heme content of the cytochrome. The cytochrome could be split by acid acetone into protoheme and an apoprotein moiety. The recombination of the two components could be observed spectro-photometrically, but the reconstructed cytochrome was no more native as evidenced by its capabiliiy to combine with CO. The normal oxidation-reduction potential of purified cytochrome bt was determined to be about -20 mV at pH 7.0 and 25°C. Spectral properties, reactivity and chemical composition of the purified preparation were also studied.We wish to thank Dr. A. Ohsaka of the Natio-nal Institute of Health, Tokyo, for a generous gift of snake venom, and Dr. K. Kakiuchi of the Institute for Protein Research, Osaka University, Osaka, for carrying out the ultracentrifugal analyses.
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