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論文 |
論文
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Ando, Hitoshi ; Takamura, Toshinari ; Matsuzawa-Nagata, Naoto ; Shima, Kosuke R. ; Nakamura, Seiji ; Kumazaki, Masafumi ; Kurita, Seiichiro ; Misu, Hirofumi ; Togawa, Naoyuki ; Fukushima, Tatsunobu ; Fujimura, Akio ; Kaneko, Shuichi
概要:
金沢大学医薬保健研究域医学系<br />Recent studies have correlated metabolic diseases, such as metabolic syndrome and non-alcoholic fatt
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y liver disease, with the circadian clock. However, whether such metabolic changes per se affect the circadian clock remains controversial. To address this, we investigated the daily mRNA expression profiles of clock genes in the liver of a dietary mouse model of non-alcoholic steatohepatitis (NASH) using a custom-made, high-precision DNA chip. C57BL/6J mice fed an atherogenic diet for 5 weeks developed hypercholesterolemia, oxidative stress, and NASH. DNA chip analyses revealed that the atherogenic diet had a great influence on the mRNA expression of a wide range of genes linked to mitochondrial energy production, redox regulation, and carbohydrate and lipid metabolism. However, the rhythmic mRNA expression of the clock genes in the liver remained intact. Most of the circadianly expressed genes also showed 24-h rhythmicity. These findings suggest that the biological clock is protected against such a metabolic derangement as NASH. © 2009 Elsevier Inc. All rights reserved.
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論文 |
論文
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Ando, Hitoshi ; Oshima, Yasuo ; Yanagihara, Hayato ; Hayashi, Yohei ; Takamura, Toshinari ; Kaneko, Shuichi ; Fujimura, Akio
概要:
金沢大学大学院医学系研究科環境社会医学<br />Although a number of genes expressed in most tissues, including the liver, exhibit circadian re
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gulation, gene expression profiles are usually examined only at one scheduled time each day. In this study, we investigated the effects of obese diabetes on the hepatic mRNA levels of various genes at 6-h intervals over a single 24-h period. Microarray analysis revealed that many genes are expressed rhythmically, not only in control KK mice but also in obese diabetic KK-Ay mice. Real-time quantitative PCR verified that 19 of 23 putative circadianly expressed genes showed significant 24-h rhythmicity in both strains. However, obese diabetes attenuated these expression rhythms in 10 of 19 genes. More importantly, the effects of obese diabetes were observed throughout the day in only two genes. These results suggest that observation time influences the results of gene expression analyses of genes expressed circadianly. © 2006 Elsevier Inc. All rights reserved.
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