1.

論文
論文
1. Effect of Kaolinite on Microbial Growth in High Concentration of Heavy Oil
Chaerun, S. Khodijah ; Tazaki, Kazue
出版情報: Clay Science.  12  pp.187-196,  2003-01-01.  日本粘土学会 = The Clay Science Society of Japan
URL: http://hdl.handle.net/2297/48700
概要: 出版者照会後に全文公開<br />The effect of kaolinite on the growth of hydrocarbon-utilizing indigenous microorganisms in high concen … tration of heavy oil (i.e., approximately 150 g/l) was studied. Microorganisms were able to grow well in such an extremely high concentration in the presence of yeast extract as co-substrate in direct contact with kaolinite. The presence of kaolinite was not toxic but stimulant for the microbial growth associated with the formation of biofilms at pH values of 5-6 after 36 days of incubation. Bacterial growth predominated when the pH of solutions was neutral-alkaline condition, while fungal growth was predominant as the pH of solutions decreased to be as low as 5 or lower. TEM observation of the kaolinite-oil-microorganism complexes showed that the microbial cells tended to be primarily bound on the edges of kaolinite, and XRD analysis confirmed that their complexes were the adsorption of the cells and heavy oil to the external surfaces of kaolinite. The results may contribute to what environmental factors having a great influence on the bioremediation process is therefore fundamental to many areas contaminated with oil spills, primarily marine and coastal environments. To our knowledge, there are no previous reports on the use of the clay minerals (i.e., kaolinite) in the bioremediation of the Nakhodka oil spill in combination with biofilm formation. 続きを見る
2.

論文
論文
2. Heavy metallic concentration in microbial mats found at hydrothermal systems, Kamchatka, Russia
Tazaki, Kazue ; Okrugin, Victor M ; Okuno, Masayuki ; Belkova, Natalia ; Islam, ABM Rafiqul ; Chaerun, S. Khodijah ; Wakimoto, Rie ; Sato, Kazuhiro ; Moriichi, Shingo
出版情報: The science reports of the Kanazawa University＝金沢大学理科報告.  47(01_02)  pp.1-48,  2003-01-01. 
URL: http://hdl.handle.net/2297/3755
概要: Department of Earth Sciences, Faculty of Science, Kanazawa University<br />Institute of Volcanology Far Eastern Division … of Russian Academy of Sciences<br />Graduate School of Natural Science and Technology, Kanazawa University<br />This study described the investigation of microbial mats that are rich in iron, arsenic, and manganese in four hydrothermal systems of Kamchatka, Russia namely Vilyuchinskie, Mutnovskie, Nachikinskie, and Malkinskie. The hydrothermal systems (hot springs) are contributing to the metallic and non-metallic mineral resources of Russia such as oil, gas, coal, copper, nickel, cobalt, tin, mercury, lead, zinc, diamond, platinum, gold, and silver. We observed the biogeochemical activities of microorganisms originating from microbial mats. The structure and elemental composition of microbial mats in these hydrothermal systems were studied with optical microscopy and scanning electron microscopy equipped with energy dispersive X-ray analyzer (EDX), whereas the water quality of these springs was measured by using pack tests. Additionally, portable Y-ray analyzer was employed to determine the kind and quantity of Y-ray in the atmospheric condition of sampling areas. Optical and scanning electron microscopic observations revealed that the microbial mats at these springs were mainly composed of a large number of microorganisms such as bacteria (coccus, bacillus, and filamentous), cyanobacteria, and algae in association with biominerals. Bacterial fluorometric enumeration of the thermal water informed that the total number of bacteria was relatively low, while the fraction of enzymatically active bacteria was high ranging from 27 % to 91 %. Besides that, ƒﾁ-ray observation showed that the predominantly ƒﾁ-ray range was between 320-380 keV dominating in green and black-colored microbial mats at Vilyuchinskie hot springs. Correspondingly, heavy metal and minerals deposits accumulated at all these springs indicating that microorganisms may contribute to binding and formation of the minerals. These activities and heavy mineral encrustation of cyanobacteria, bacteria, and algae may contribute to the growth of the heavy metal deposit (such as iron, manganese, and arsenic) at these springs. Obviously, Kamchatka hot springs provide a model for studying the potential role of prokaryotes and eukaryotes in the origin of heavy metal and minerals formation. 続きを見る
3.

論文
論文
3. 重油汚染環境における微生物の環境修復法の研究
田崎, 和江 ; Tazaki, Kazue
出版情報: 平成14(2002)年度 科学研究費補助金 基盤研究(A) 研究成果報告書 = 2002 Fiscal Year Final Research Report.  1999-2002  pp.7p.-,  2003-03-01.  金沢大学理学部
URL: http://hdl.handle.net/2297/47781
概要: 1997年1月2日、約12,000kLのC重油を積んだロシア船籍タンカー<ナホトカ号>が隠岐島沖で分断して沈没した。船首部は北西の強風を受け漂流した後、1月7日、福井県三国沖で座礁し、深刻な海洋汚染を北陸沿岸にもたらした。北陸沿岸で海水,砂 … を含む約30,000kLの重油が回収されたが、その後も流出が続き、1,300kmに渡って海岸が汚染された。 5年半たった現在も珠洲市シャク崎、長橋、輪島市大沢、アタケ海岸、千枚田海岸などで重油が目視できる。昨年から今年にかけて新たに漂着したと見られる油塊も認められた。2001年11月にはアタケ海岸において重油の入った砂袋10kgが回収されたが、粘着性の高い、黒茶色の油塊であった。海水に頻繁に洗われる環境では物理的洗浄による油の除去が行われると共に微生物によるバイオレメディエーションが進むが、砂海岸のように有機物の少ない環境では分解速度が遅いことが明らかとなった。 自然培養実験とバイオレメディエーションの可能性についても研究を行った。粘土、砂、水苔、酸性白土、鹿沼土などを入れた容器に、海水とナホトカ号のC重油を滴下した目視実験は、5年半を経過した現在、それぞれの環境条件により重油の分解に大きな差異が認められた。容器を野外と室内に設置した場合、野外の方が重油の分解が早く、油塊がほとんど認められないのに対し、室内の容器にはまだ油塊が存在する。また、砂のみの場合は分解が遅いが、水苔、酸性白土、鹿沼土など入れた場合は分解が早く、かつ、海水と河川水とでは、海水の方が油の分解が早いことが明らかとなった。分解速度は太陽光線、有機物量の存在量と供給量、水質に依存する。それぞれの容器中のオイルスリックには多くの重油分解細菌の増殖が認められた。野外において重油の容器は紫外線による分解の他、微生物によりさらに分解が加速されることが明らかになった。<br />The Nakhodka, a Russian tanker loaded with C-typed heavy oil of 19,000 kl was divided into sections and submerged off Oki Island, Shimane Prefecture on January 2,1997. The bow, after being drifting for 4 days, was wrecked off Anto, Mikuni town, Fukui Prefecture, threatened throughout the various shores of Ishikawa Prefecture geographically as well. Shore damage by oil washing was observed by aerial research of K. Tazaki, as of 8 Feb. 1997. An oil layer at the shore of Mikuni Town reached over 50 cm in thickness. We conducted field surveys in order to examine the conditions of heavy oil washing ashore on the Ishikawa coastline, in this accident, remarkable microbial remediation research had been advanced. In fact, hydrocarbon-degrading microbes were found in each polluted area of the near-shore environments. Hydrocarbon-utilizing bacteria isolated from coastal areas. Local degrader bacteria inhibited everywhere with many kinds of species. Each species carries a role ta resolve heavy oil under the local conditions. That is a key to understand the sustainable "bioremediation " for polluted local area. After 5 years of spilled accident, degrader bacteria were still alive to keep the activity.<br />研究課題/領域番号:11354006, 研究期間(年度):1999–2002<br />出典：「重油汚染環境における微生物の環境修復法の研究」研究成果報告書　課題番号11354006　　　本文データは著者版報告書より作成 続きを見る
4.

論文
論文
4. Preface
Tazaki, Kazue
出版情報: "Heavy oil spilled from Russian tanker ""Nakhodoka"" in 1998 : Towards eco-responsibility, eartb sense".  pp.1-2,  2003-10-01.  金沢大学 — 21st Century COE Kanazawa University, Long- and Short- term Dynamics of Pan-Japan Sea Area Environmental Monitoring and Predicition, Program Leader, Kazuichi Hayakawa, October 2003, Kanazawa, Japan — 金沢大学21世紀COEプログラム「日本海域の環境計測と長期・短期変動予測」
URL: http://hdl.handle.net/2297/5519
概要: 金沢大学大学院自然科学研究科　環境科学<br />Editor : Tazaki, Kazue |田崎, 和江
5.

論文
論文
5. The environmental impacts on heavy oil spilled from the wrecked Russian tanker Nakhodka attacked the coast of Hokuriku direct, Japan, in 1997,pp.1-461
Tazaki, Kazue ; Tazaki's seminar
出版情報: "Heavy oil spilled from Russian tanker ""Nakhodoka"" in 2002 : Towards eco-responsibility, eartb sense".  pp.1-46,  2003-10-01.  金沢大学 — 21st Century COE Kanazawa University, Long- and Short- term Dynamics of Pan-Japan Sea Area Environmental Monitoring and Predicition, Program Leader, Kazuichi Hayakawa, October 2003, Kanazawa, Japan — 金沢大学21世紀COEプログラム「日本海域の環境計測と長期・短期変動予測」
URL: http://hdl.handle.net/2297/5521
概要: 金沢大学大学院自然科学研究科　環境科学<br />Editor : Tazaki, Kazue |田崎, 和江
6.

論文
論文
6. Compositional changes of heavy oil and aliphatic hydrocarbon from the spilled NAKHODKA-Oil washed asore at Fukui, Ishikawa and Niigata Prefectures, Japan
Sampei, Yoshikazu ; Tazaki, Kazue ; Obara, Yoshihiro ; Yoshimura, Takahisa ; Sawano, Nobuhiro ; Takayasu, Katsumi ; Iizumi, Shigeru ; Tokuoka, Takao
出版情報: "Heavy oil spilled from Russian tanker ""Nakhodoka"" in 1997 : Towards eco-responsibility, eartb sense,pp.174-192".  pp.174-192,  2003-10-01.  金沢大学 — 21st Century COE Kanazawa University, Long- and Short- term Dynamics of Pan-Japan Sea Area Environmental Monitoring and Predicition, Program Leader, Kazuichi Hayakawa, October 2003, Kanazawa, Japan — 金沢大学21世紀COEプログラム「日本海域の環境計測と長期・短期変動予測」
URL: http://hdl.handle.net/2297/5532
概要: 島根大学<br />金沢大学大学院自然科学研究科 環境科学<br />Editor : Tazaki, Kazue |田崎, 和江
7.

論文
論文
7. Various styles and long-term chemical changes of the heavy oil spilled from "Nakhodka"
Nishikawa, Osamu ; Sawano, Nobuhiro ; Hasegawa, Takashi ; Tazaki, Kazue
出版情報: "Heavy oil spilled from Russian tanker ""Nakhodoka"" in 2010 : Towards eco-responsibility, eartb sense".  pp.192-202,  2003-10-01.  金沢大学 — 21st Century COE Kanazawa University, Long- and Short- term Dynamics of Pan-Japan Sea Area Environmental Monitoring and Predicition, Program Leader, Kazuichi Hayakawa, October 2003, Kanazawa, Japan — 金沢大学21世紀COEプログラム「日本海域の環境計測と長期・短期変動予測」
URL: http://hdl.handle.net/2297/5533
概要: 金沢大学大学院自然科学研究科 環境科学<br />Editor : Tazaki, Kazue |田崎, 和江
8.

論文
論文
8. Double function of bentonite and kaolinite as adsorbents and "Microbial growth-support media" for degradation of crude oil
Chaerun, S. Khodijah ; Tazaki, Kazue ; Asada, Ryuji
出版情報: "Heavy oil spilled from Russian tanker ""Nakhodoka"" in 2007 : Towards eco-responsibility, eartb sense".  pp.253-277,  2003-10-01.  金沢大学 — 21st Century COE Kanazawa University, Long- and Short- term Dynamics of Pan-Japan Sea Area Environmental Monitoring and Predicition, Program Leader, Kazuichi Hayakawa, October 2003, Kanazawa, Japan — 金沢大学21世紀COEプログラム「日本海域の環境計測と長期・短期変動予測」
URL: http://hdl.handle.net/2297/5538
概要: Editor : Tazaki, Kazue |田崎, 和江
9.

論文
論文
9. Microorganizms living in emulsified heav oil
Tawara, Kenji ; Tazaki, Kazue
出版情報: "Heavy oil spilled from Russian tanker ""Nakhodoka"" in 2005 : Towards eco-responsibility, eartb sense".  pp.309-315,  2003-10-01.  金沢大学 — 21st Century COE Kanazawa University, Long- and Short- term Dynamics of Pan-Japan Sea Area Environmental Monitoring and Predicition, Program Leader, Kazuichi Hayakawa, October 2003, Kanazawa, Japan — 金沢大学21世紀COEプログラム「日本海域の環境計測と長期・短期変動予測」
URL: http://hdl.handle.net/2297/5542
概要: 金沢大学大学院自然科学研究科　環境科学<br />Editor : Tazaki, Kazue | 田崎, 和江
10.

論文
論文
10. Bioremediation of oil contaminated soils -ultivation experoments using an oil adsorption-resolution item "Sponge"-
Asada, Ryuji ; Tanaka, Yoshitaro ; Chaerun, S. Khodijah ; Tazaki, Kazue
出版情報: "Heavy oil spilled from Russian tanker ""Nakhodoka"" in 1997 : Towards eco-responsibility, eartb sense".  pp.337-351,  2003-10-01.  金沢大学 — 21st Century COE Kanazawa University, Long- and Short- term Dynamics of Pan-Japan Sea Area Environmental Monitoring and Predicition, Program Leader, Kazuichi Hayakawa, October 2003, Kanazawa, Japan — 金沢大学21世紀COEプログラム「日本海域の環境計測と長期・短期変動予測」
URL: http://hdl.handle.net/2297/5545
概要: 金沢大学大学院自然科学研究科　環境科学<br />Editor : Tazaki, Kazue |田崎, 和江