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Avner Friedman (ed.) ; with contributions by B. Aguda ... [et al.]
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論文 |
論文
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Ogai, Kazuhiro ; Nakatani, Kumi ; Hisano, Suguru ; Sugitani, Kayo ; Koriyama, Yoshiki ; Kato, Satoru
概要:
The sex-determining region Y-box 2 (Sox2) is related not only to pluripotency, but also to cell proliferation. Zebrafish
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can regain their motor function after spinal cord injury (SCI). Following SCI, new motor neurons are produced from proliferating ependymal cells. Here, we investigated the expression and function of Sox2 after SCI in zebrafish. Sox2 was upregulated as early as 1 day post-lesion (dpl) in ependymal cells, which was followed by cell proliferation. Sox2 knockdown significantly decreased the number of proliferating cells at 5 dpl. The results of this study suggest a role of Sox2 as one of the proliferation initiators in ependymal cells after SCI. © 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society.<br />12 months Embargo Period
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論文 |
論文
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Ogai, Kazuhiro ; Hisano, Suguru ; Sugitani, Kayo ; Koriyama, Yoshiki ; Kato, Satoru
概要:
Zebrafish can regenerate several organs such as the tail fin, heart, central nervous system, and photoreceptors. Very re
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cently, a study has demonstrated the photoreceptor regeneration in the alkylating agent N-methyl-N-nitrosourea (MNU)- induced retinal degeneration (RD) zebrafish model, in which whole photoreceptors are lost within a week after MNU treatment and then regenerated within a month. The research has also shown massive proliferation of Müller cells within a week. To address the question of whether proliferating Müller cells are the source of regenerating photoreceptors, which remains unknown in the MNU-induced zebrafish RD model, we employed a BrdU pulse-chase technique to label the proliferating cells within a week after MNU treatment. As a result of the BrdU pulse-chase technique, a number of BrdU+ cells were observed in the outer nuclear layer as well as the inner nuclear layer. This implies that regenerating photoreceptors are derived from proliferating Müller cells in the zebrafish MNU-induced RD model. © Springer International Publishing Switzerland 2016.<br />[Book Chapter]
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論文 |
論文
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Ogai, Kazuhiro ; Nakatani, Kumi ; Hisano, Suguru ; Sugitani, Kayo ; Koriyama, Yoshiki ; Kato, Satoru
概要:
The sex-determining region Y-box 2 (Sox2) is related not only to pluripotency, but also to cell proliferation. Zebrafish
…
can regain their motor function after spinal cord injury (SCI). Following SCI, new motor neurons are produced from proliferating ependymal cells. Here, we investigated the expression and function of Sox2 after SCI in zebrafish. Sox2 was upregulated as early as 1 day post-lesion (dpl) in ependymal cells, which was followed by cell proliferation. Sox2 knockdown significantly decreased the number of proliferating cells at 5dpl. The results of this study suggest a role of Sox2 as one of the proliferation initiators in ependymal cells after SCI. Copyright © 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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論文 |
論文
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Takeichi, Toshiaki ; Takarada-Iemata, Mika ; Hashida, Koji ; Sudo, Hirofumi ; Okuda, Tomohiko ; Kokame, Koichi ; Hatano, Taku ; Takanashi, Masashi ; Funabe, Sayaka ; Hattori, Nobutaka ; Kitamura, Osamu ; Kitao, Yasuko ; Hori, Osamu
概要:
N-myc downstream-regulated gene 2 (Ndrg2) is a differentiation- and stress-associated molecule predominantly expressed i
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n astrocytes in the central nervous system (CNS). To study the expression and possible role of Ndrg2 in quiescent and activated astrocytes, mice were administrated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropypridine (MPTP), a Parkinson disease (PD)-related neurotoxin which causes both neurodegeneration and glial activation. Immunohistological analysis revealed that Ndrg2 was highly expressed in both types of astrocytes, but less so in astrocytes during the early process of activation. Ndrg2 was also expressed in astrocyte-like cells, but not in neurons, in human brains from PD and Cortico-basal degeneration (CBD) patients. In cultured astrocytes, gene silencing of Ndrg2 significantly enhanced the numbers of 5-bromo-2′-deoxy-uridine (BrdU)-incorporated and proliferating cell nuclear antigen (PCNA)-positive cells, and reduced the length of cell processes and the amount of F-actin. In contrast, adenovirus-mediated overexpression of Ndrg2 significantly reduced the numbers of BrdU-incorporated and PCNA-positive cells, and enhanced the amount of F-actin. Fractionation and immunocytochemical analysis further revealed that Ndrg2 was located in different cellular fractions including the cytosol and cell surface membranes. These results suggest that Ndrg2 may regulate astroglial activation through the suppression of cell proliferation and stabilization of cell morphology. © 2011 Elsevier Ltd. All rights reserved.
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