※一部利用できない機能があります
| 1.
その他 |
その他
|
二宮, 致 ; Ninomiya, Itasu
概要:
取得学位 : 博士（医学）, 学位授与番号 : 医博甲第1115号, 学位授与年月日：平成6年3月25日,学位授与年：1994
|
||||
| 2.
論文 |
論文
|
Watanabe, Toshifumi ; Tajima, Hidehiro ; Hayashi, Hironori ; Nakagawara, Hisatoshi ; Ohnishi, Ichiro ; Takamura, Hiroyuki ; Ninomiya, Itasu ; Kitagawa, Hirohisa ; Fushida, Sachio ; Tani, Takashi ; Fujimura, Takashi ; Ohta, Tetsuo ; Wakayama, Tomohiko ; Iseki, Shoichi ; Harada, Shinichi ; 田島, 秀浩 ; 林, 泰寛 ; 中川原, 寿俊 ; 高村, 博之 ; 二宮, 致 ; 北川, 裕久 ; 伏田, 幸夫 ; 谷, 卓 ; 藤村, 隆 ; 太田, 哲生 ; 若山, 友彦 ; 井関, 尚一 ; 原田, 真市
概要:
金沢大学医薬保健研究域医学系<br />Histone acetylation and deacetylation have been thought to be related to gene expression, and there
…
are many reports indicating that histone deacetylase inhibitors (HDACis) exert antifibrogenic effects in several organs. In injured livers, hepatic stellate cells (HSCs) are activated in response to profibrogenic mediators and produce large amounts of extracellular matrix. In particular, transforming growth factor-β1 (TGF-β1) is considered as a key factor in accelerating hepatic fibrosis because it is released from activated HSCs and further stimulates them. The present study aimed to clarify whether sodium valproate (VPA) has suppressive effects on cultured human HSCs (LI90). We showed that treatment with VPA had no significantly suppressive effect on cell proliferation at a concentration of 1 mM, which corresponded approximately to the serum concentration obtained by the administration of a clinical dose. However, VPA prevented the morphological changes characteristic for activation and inhibited the expression of collagen type 1 α1 (COL1A1) and TGF-β1 in activated LI90 cells at the mRNA and protein levels. Our results support the hypothesis that VPA exerts antifibrogenic activity with little cytotoxicity at 1 mM, and HDACis are expected to be used in clinical practice for the treatment of fibrotic diseases.<br />Embargo Period 6 months
続きを見る
|
||||
| 3.
論文 |
論文
|
Okamoto, Koichi ; Tajima, Hidehiro ; Nakanuma, Shinichi ; Sakai, Seisho ; Makino, Isamu ; Kinoshita, Jun ; Hayashi, Hironori ; Nakamura, Keishi ; Oyama, Katsunobu ; Nakagawara, Hisatoshi ; Fujita, Hideto ; Takamura, Hiroyuki ; Ninomiya, Itasu ; Kitagawa, Hirohisa ; Fushida, Sachio ; Fujimura, Takashi ; Harada, Shinichi ; Wakayama, Tomohiko ; Iseki, Shoichi ; Ohta, Tetsuo ; 岡本, 浩一 ; 田島, 秀浩 ; 中村, 信一 ; 牧野, 勇 ; 木下, 淳 ; 林, 泰寛 ; 中村, 慶史 ; 尾山, 勝信 ; 中川原, 寿俊 ; 藤田, 秀人 ; 高村, 博之 ; 二宮, 致 ; 北川, 裕久 ; 伏田, 幸夫 ; 藤村, 隆 ; 原田, 真市 ; 若山, 友彦 ; 井関, 尚一 ; 太田, 哲生
概要:
金沢大学医薬保健研究域医学系<br />We previously reported that hepatic stellate cells (HSCs) activated by angiotensin II (AngII) facili
…
tate stromal fibrosis and tumor progression in intrahepatic cholangiocarcinoma (ICC). AngII has been known as a growth factor which can promote epithelial-to-mesenchymal transition (EMT) in renal epithelial cells, alveolar epithelial cells and peritoneal mesothelial cells. However, in the past, the relationship between AngII and stromal cell-derived factor-1 (SDF-1) in the microenvironment around cancer and the role of AngII on EMT of cancer cells has not been reported in detail. SDF-1 and its specific receptor, CXCR4, are now receiving attention as a mechanism of cell progression and metastasis. In this study, we examined whether activated HSCs promote tumor fibrogenesis, tumor progression and distant metastasis by mediating EMT via the AngII/AngII type 1 receptor (AT-1) and the SDF-1/CXCR4 axis. Two human ICC cell lines and a human HSC line, LI-90, express CXCR4. Significantly higher concentration of SDF-1αwas released into the supernatant of LI-90 cells to which AngII had been added. SDF-1α increased the proliferative activity of HSCs and enhanced the activation of HSCs as a growth factor. Furthermore, addition of SDF-1α and AngII enhanced the increase of the migratory capability and vimentin expression, reduced E-cadherin expression, and translocated the expression of β-catenin into the nucleus and cytoplasm in ICC cells. Co-culture with HSCs also enhanced the migratory capability of ICC cells. These findings suggest that SDF-1α, released from activated HSCs and AngII, play important roles in cancer progression, tumor fibrogenesis, and migration in autocrine and paracrine fashion by mediating EMT. Our mechanistic findings may provide pivotal insights into the molecular mechanism of the AngII and SDF-1α-initiated signaling pathway that regulates fibrogenesis in cancerous stroma, tumor progression and metastasis of tumor cells expressing AT-1 and CXCR4.<br />Embargo Period 6 months
続きを見る
|
||||
| 4.
論文 |
論文
|
Tajima, Hidehiro ; Takamura, Hiroyuki ; Kitagawa, Hirohisa ; Nakayama, Akira ; Shoji, Masatoshi ; Watanabe, Toshifumi ; Tsukada, Tomoya ; Nakanuma, Shinichi ; Okamoto, Koichi ; Sakai, Seisho ; Kinoshita, Jun ; Makino, Isamu ; Nakamura, Keishi ; Hayashi, Hironori ; Oyama, Katsunobu ; Inokuchi, Masafumi ; Nakagawara, Hisatoshi ; Miyashita, Tomoharu ; Ninomiya, Itasu ; Fushida, Sachio ; Fujimura, Takashi ; Wakayama, Tomohiko ; Iseki, Shoichi ; Ikeda, Hiroko ; Ohta, Tetsuo ; 田島, 秀浩 ; 高村, 博之 ; 北川, 裕久 ; 中村, 信一 ; 岡本, 浩一 ; 木下, 淳 ; 牧野, 勇 ; 中村, 慶史 ; 林, 泰寛 ; 尾山, 勝信 ; 井口, 雅史 ; 中川原, 寿俊 ; 宮下, 知治 ; 二宮, 致 ; 若山, 友彦 ; 藤村, 隆 ; 井関, 尚一 ; 池田, 博子 ; 太田, 哲生
概要:
金沢大学医薬保健研究域医学系<br />A 33-year-old female was diagnosed with a solid pseudopapillary tumor (SPT) of the pancreas and mult
…
iple liver metastases at the Department of Gastroenterological Surgery, Ishikawa Prefectural Central Hospital (Kanazawa, Japan). Distal pancreatectomy and postoperative systemic chemotherapy with gemcitabine (GEM) and S-1, an oral fluoropyrimidine derivative, was administered, however, liver metastases became enlarged and local recurrence occurred. Therefore, the patient was referred to the Department of Gastroenterologic Surgery at the Graduate School of Medicine (Kanazawa, Japan) for hepatic arterial infusion (HAI) chemotherapy. Oral S-1 (80 mg/m2) was administered as well as HAI chemotherapy with GEM (1,000 mg/standard liver volume). Following 18 cycles, tumor sizes were reduced and 18-fluorodeoxyglucose positron emission tomography (18FDG-PET) examination revealed obvious reduction of tumor FDG uptake. Transarterial tumor embolization (TAE) was performed for the previously unresectable right subphrenic liver tumor, and the other tumors were surgically resected. The resected tumors were diagnosed as liver metastases and a local recurrence of SPT in the postoperative pathological examination, which revealed that the resected tumors were composed of sheets of bland cells, which were positive for CD10, CD56, vimentin, neuron-specific enolase and α-antitrypsin. The postoperative course was uneventful, and the patient is currently under observation at an outpatient clinic; postoperative adjuvant chemotherapy with oral S-1 has continued, and additional TAE is planned. In the future, if the middle segment of the liver becomes enlarged, surgery for the residual right lobe tumor may be possible. This case demonstrates one method of SPT treatment: Preoperative HAI chemotherapy with GEM, plus oral S-1 and TAE. If complete resection can be achieved, the majority of patients with SPT have a favorable prognosis. In patients with unresectable metastases from SPT, it is crucial to conduct systematic multimodal treatment to maximize treatment success. © 2015, Spandidos Publications. All Rights Reserved.<br />Embargo Period 6 months
続きを見る
|
||||
| 5.
論文 |
論文
|
Yoshimoto, Katsuhiro ; Tajima, Hidehiro ; Ohta, Tetsuo ; Okamoto, Koichi ; Sakai, Seisho ; Kinoshita, Jun ; Furukawa, Hiroyuki ; Makino, Isamu ; Hayashi, Hironori ; Nakamura, Keishi ; Oyama, Katsunobu ; Inokuchi, Masafumi ; Nakagawara, Hisatoshi ; Itoh, Hiroshi ; Fujita, Hideto ; Takamura, Hiroyuki ; Ninomiya, Itasu ; Kitagawa, Hirohisa ; Fushida, Sachio ; Fujimura, Takashi ; Wakayama, Tomohiko ; Iseki, Shoichi ; Shimizu, Koichi ; 田島, 秀浩 ; 太田, 哲生 ; 岡本, 浩一 ; 木下, 淳 ; 古川, 裕之 ; 牧野, 勇 ; 林, 泰寛 ; 中村, 慶史 ; 尾山, 勝信 ; 井口, 雅史 ; 中川原, 寿俊 ; 藤田, 秀人 ; 高村, 博之 ; 二宮, 致 ; 藤村, 隆 ; 若山, 友彦 ; 井関, 尚一 ; 清水, 康一
概要:
金沢大学医薬保健研究域医学系<br />Several recent studies have reported that selectins are produced during ischemia-reperfusion injury,
…
and that selectin ligands play an important role in cell binding to the endothelium and in liver metastasis. Portal clamping during pancreaticoduodenectomy with vessel resection for pancreatic head cancer causes hepatic ischemia-reperfusion injury, which might promote liver metastasis. We investigated the liver colonization of pancreatic cancer cells under hepatic ischemia-reperfusion and examined the involvement of E-selectin and its ligands. A human pancreatic cancer cell line (Capan-1) was injected into the spleen of mice after hepatic ischemia-reperfusion (I/R group). In addition, to investigate the effect of an anti-E-selectin antibody on liver colonization in the IR group, mice received an intraperitoneal injection of the anti-E-selectin antibody following hepatic ischemia-reperfusion and tumor inoculation (IR+Ab group). Four weeks later, mice were sacrificed and the number of tumor nodules on the liver was compared to mice without hepatic ischemia-reperfusion (control group). The incidence of liver metastasis in the I/R group was significantly higher (16 of 20, 80%) than that in the control group (6 of 20, 30%) (P<0.01). Moreover, mice in the I/R group had significantly more tumor nodules compared to those in the control group (median, 9.9 vs. 2.7 nodules) (P<0.01). In the I/R+Ab group, only 2 of 5 (40%) mice developed liver metastases. RT-PCR and southern blotting of the liver extracts showed that the expression of IL-1 and E-selectin mRNA after hepatic ischemia-reperfusion was significantly higher than the basal levels. Hepatic ischemia-reperfusion increases liver metastases and E-selectin expression in pancreatic cancer. These results suggest that E-selectin produced due to hepatic ischemia-reperfusion is involved in liver metastasis.<br />Embargo Period 6 months
続きを見る
|
||||
| 6.
論文 |
論文
|
二宮, 致
|
||||
| 7.
論文 |
論文
|
能登, 正浩 ; 二宮, 致 ; 佐々木, 省三 ; 西村, 元一 ; 藤村, 隆 ; 萱原, 正都 ; 清水, 康一 ; 太田, 哲生 ; 三輪, 晃一
概要:
症例は32歳女性.主訴は上腹部痛と嘔吐.内視鏡検査で十二指腸の閉塞と潰瘍を認めた.低緊張性十二指腸造影で十二指腸下行部に内腔に突出する袋状陰影を認め,その周囲に薄い透明帯を呈した.十二指腸内憩室(IDD)と診断し,内視鏡的憩室切除術を施行し
…
た.合併症は認めず,閉塞症状は改善した.IDDは比較的稀な消化管奇形であり,本邦報告例は本例で45例日である.治療は内視鏡的切除術が第一選択と考えられた. A 32-year-01d woman visited our hospital complaining of postprandial epigastralgia andvomiting. She had occasionally had the same symptom from childhood. Hypotonic duQdenography revealed a barium-filled pear-shaped sac surrounded by thinradiolucent line in the second part of duodenum. The findings are most suggestive ofintraluminal duodenal diverticulum.(IDD). We performed an endoscopic excision of IDD with2-channel fiberscape. After inversion of the diverticular sac to the oral side by forceps, sac wasexcised by polybectom.y-snare with a high voltage electric current. Histological findings of theexcised specimen revealed normal duodenal mucosa on both sides of the sac. After this excisionshe obtained eomplete relief of the symptom. IDD is a rare congenital anomaly of the duodenum As far as we know, 45 cases of IDDincluding our case have been reported in Japan. furthermore, endoscopic removal of thediverticular sac would be a minimal invasive and effective method in the rnanagement of IDD.
続きを見る
|
||||
| 8.
論文 |
論文
|
中沼, 伸一 ; 木南, 伸一 ; 尾山, 勝信 ; 舟木, 洋 ; 藤田, 秀人 ; 二宮, 致 ; 伏田, 幸夫 ; 藤村, 隆 ; 萱原, 正都 ; 太田, 哲生
概要:
症例は58歳の女性で,多発性骨髄腫の化学療法中にCTにて胃体上部の壁肥厚と壁内に多数の嚢胞性病変を指摘された.胃透視検査では体上部から穹窿部に境界不明瞭な壁の硬化を認め,胃内視鏡検査所見は胃体上部後壁を中心とした境界不明瞭な丈の低い結節状隆
…
起を呈していた.組織診は高分化型の腺癌であった.多発性胃粘膜下嚢腫を伴ったBorrmann IV型の進行胃癌と診断し手術を施行した.癌は体上部から穹窿部全体に浸潤し,漿膜露出と腹膜播種も認めた.病理組織学的検査所見は明瞭な腺腔形成を示す高分化型の腺癌で繊維化を伴いびまん浸潤していた.免疫染色検査ではMUC2陰性,MUC5AC・MUC6陽性で胃型であった.高分化型のBorrmann IV型胃癌はまれである.本症例では拡張した癌腺管と,境界不明瞭で丈の低い結節状隆起を示した内視鏡像が特異であり,前者は高分化型Borrmann IV型癌に,後者は胃型の分化型癌に関係した性質と考えられた. A 58-year-old woman admitted for multiple myeloma and treated with chemotherapy was found in Abdominal computed tomography (CT) to have showed wall thickening in the upper gastric body, together with multiple cystic lesions at the same site. Barium enema studies showed wall hardening with unclear margins in the upper gastric body and fundus. Endoscopy showed a low protruding nodular lesion with unclear margins on the posterior wall of the upper gastric body. Endoscopic biopsy indicated well-differentiated adenocarcinoma, yielding A diagnosis of Borrmann type 4 advanced gastric cancer with diffuse heterotopic multiple cysts. The tumor was found to have spread from the upper gastric body to the fundus, with serosal penetration and peritoneal dissemination. The Pathological diagnosis was well-differentiated adenocarcinoma invading all layers diffusely forming large glandular cavities and scirrhous changes. Immunohistochemical staining showed tumor cells to be positive for MUC5AC and slightly positive for MUC6 but negative for MUC2. Based on these findings, the definitive diagnosis was gastric phenotype. Borrmann type 4 advanced gastric cancer consisting of well-differentiated adenocarcinoma alone is rare. Our case suggested two characteristic features: large carcinomatous glandular cavities such as gastric cysts related to well-differentiated adenocarcinoma of Borrmann type 4 advanced gastric cancer, and an endoscopic picture of a low protruding nodular lesion with unclear margins related to differentiated carcinoma with a gastric phenotype.
続きを見る
|
||||
| 9.
論文 |
論文
|
山崎, 祐樹 ; 伏田, 幸夫 ; 尾山, 勝信 ; 木下, 淳 ; 牧野, 勇 ; 中村, 慶史 ; 藤田, 秀人 ; 二宮, 致 ; 藤村, 隆 ; 太田, 哲生
概要:
We report a case of advanced gastric cancer with para-aortic lymph node metastasis that underwent radical gastrectomy af
…
ter neoadjuvant chemotherapy, and in this case, thoracoscopy was useful in excluding mediastinal lymph node metastasis. The patient was a 70-year-old man in whom a large type 2 advanced gastric cancer accompanied with para-aortic and mediastinal lymph node metastasis was diagnosed. We attempted combination therapy of Docetaxel, CDDP and TS-1. After 2 courses of treatment, the primary lesion and regional and para-aortic lymph nodes were significantly reduced in size, suggesting that this therapy had induced a partial response (PR). However, the size of mediastinal lymph node did not change. FDG-PET showed accumulation of FDG located in the mediastinal lymph node, in which metastasis was suspected. To judge the possibility of radical excision, we performed tho-racoscopic biopsy of the mediastinal lymph node. No metastasis was shown in the mediastinal lymph node biopsy, so we performed curative total gastrectomy with D3 lymph node dissection and cholecys-tectomy with curative intent. The pathological findings showed that there were very few cancer cells either in the primary lesion and only one lymph node (No.3). There has been no recurrence for two years after the operation. © 2012 The Japanese Society of Gastroenterological Surgery. 術前DCS（docetaxel，cisplatin，TS-1併用）療法が著効した大動脈周囲リンパ節転移を伴う進行胃癌を経験した．また，胸腔鏡下生検が縦隔リンパ節転移の除外診断に有用であったので報告する．症例は70歳の男性で，噴門直下から角上部に及ぶ大型の2型腫瘍を認めた．大動脈周囲・縦隔を含め多数のリンパ節腫大を認めた．DCS療法を2コース行い，原発巣・リンパ節転移巣ともに著明に縮小した．しかし，縦隔リンパ節に変化はなくPET-CTでも淡い集積を認め転移も否定できないため，胸腔鏡下に縦隔リンパ節生検を行ったところ，転移を認めなかった．根治切除可能と判断し大動脈周囲リンパ節郭清を伴う胃全摘術を行った．組織学的に腫瘍の大部分は壊死に陥り，原発巣の一部と壁在リンパ節1個にごく僅かに腫瘍細胞が残存していた．現在，術後2年が経過しているが，無再発生存中である．
続きを見る
|
||||
| 10.
論文 |
論文
|
齋藤, 裕人 ; 尾山, 勝信 ; 伏田, 幸夫 ; 柄田, 智也 ; 岡本, 浩一 ; 中沼, 伸一 ; 木下, 淳 ; 牧野, 勇 ; 中村, 慶史 ; 林, 泰寛 ; 井口, 雅史 ; 中川原, 寿俊 ; 宮下, 知治 ; 藤田, 秀人 ; 田島, 秀浩 ; 高村, 博之 ; 二宮, 致 ; 北川, 裕久 ; 藤村, 隆 ; 太田, 哲生
概要:
We report a case of gastric adenosquamous carcinoma producing granulocyte-colony stimulating factor (G-CSF). A 60-year-o
…
ld man was admitted to our hospital complaining of upper abdominal pain. Endoscopic examination revealed a large type 5 advanced gastric cancer with bleeding from the low body of stomach to the antrum, accompanied with para-aortic and mesenteric lymph node metastasis. He had marked leukocytosis, and serum levels of G-CSF were elevated. Histological diag-nosis of the biopsy specimen was adenosquamous carcinoma producing G-CSF. We attempted combination chemotherapy with docetaxel, cisplatin and S-1 (DCS). After 1 course of treatment, the primary lesion was reduced in size. However, the size of the metastatic lymph node was larger. Chemotherapy was not effective enough, and the patient died 3 months after ending chemotherapy. 症例は60歳、男性。上腹部痛を主訴に受診。上部消化管内視鏡で胃体下部から前庭部の小弯後壁に易出血性の5型胃癌を認め、画像所見で傍大動脈・腸間膜リンパ節転移を認めた。生検にて腺癌成分と扁平上皮癌成分の混在を認めた。来院時より白血球数が著明に増多し、血清G-CSF高値、生検組織の免疫組織染色で腫瘍組織のG-CSF発現を認めた。以上よりG-CSF産生胃腺扁平上皮癌と診断し、化学療法（DCS (docetaxel/ cisplatin/ S-1併用)療法）を1コース行ったところ原発巣の縮小を認めたが、転移リンパ節の増大を認めた。急激な病状悪化により3ヶ月の経過で死亡した。
続きを見る
|
