1.

論文

論文
早川, 和一 ; 西島, 美知子 ; 宮崎, 元一
出版情報: 分析化学 = Japan analyst.  31  pp.160-161,  1982-03-05.  The Japan Society for Analytical Chemistry = 日本分析化学会
URL: http://hdl.handle.net/2297/24888
概要: 金沢大学医薬保健研究域薬学系<br />A novel method for the separation of halogenated anion complexes of platinum metals by reverse d-phase ion-pair partition chromatography has been developed. The separation of RhBr_5^<2->, PdBr_4^<2->,and PtBr_6^<2-> was accomplished on a Hitachi #3050 packed column (3mm×30cm), where 30 % (v/v) acetonitrile-0.02 M phosphate buffer (pH 7) mixture containing 0.1 M tetrabutylammonium bromide and 0.025 M sodium bromide was used as a mobile phase. These anion complexes can be determined at 310nm, and as low as 5×10^<-11> mol of PtBr_6^<-2> can be determined with the peak height method which gave a straight calibration curve. Detecting wavelengths and retention times of the complexes can be altered if the complexes are formed with other halogen ions. This analytical method for micro amounts of platinum metals can remove the mutual metal interferences. 続きを見る
2.

論文

論文
大久保, 登 ; 宮崎, 元一
出版情報: 金沢大学教育開放センター紀要 = University Extension Journal of Kanazawa University.  第7号  pp.67-70,  1987-03-31.  金沢大学大学教育開放センター = Extension Institute of Kanazawa University
URL: http://hdl.handle.net/2297/24449
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論文

論文
早川, 和一 ; 山本 , 敦 ; 宮崎, 元一 ; Hayakawa, Kazuichi ; Yamamoto, Atsushi ; Miyazaki, Motoichi
出版情報: ぶんせき.  197  pp.358-363,  1991-05-01.  日本分析化学会 = The Japan Society for Analytical Chemistry
URL: http://hdl.handle.net/2297/48481
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論文

論文
早川, 和一 ; 宮崎, 元一 ; Hayakawa, Kazuichi ; Miyazaki, Motoichi
出版情報: ぶんせき.  193  pp.47-53,  1991-01-01.  日本分析化学会 = The Japan Society for Analytical Chemistry
URL: http://hdl.handle.net/2297/48480
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論文

論文
木津, 良一 ; 濱木, 路子 ; 下沢, 充子 ; 宮崎, 元一
出版情報: 臨床化学 = Japanese Journal of Clinical Chemistry.  13  pp.155-160,  1984-01-01.  日本臨床化学会 = Japan Society of Clinical Chemistry
URL: http://hdl.handle.net/2297/45660
概要: A method for the determination of platinum in biological materials by using nickel as an internal standard was established.Ten μg of nickel was added to samples which contained platinum.Nickel spiked sample was digested with nitric acid and hydrogen peroxide. Acid digest was evaporated to dryness and the residue was dissolved in 0.5ml of IM nitric acid. The amount of platinum was measured byflameless atomic absorption spectrophotometer using background correction with deuterium Iamp, Nickel was measured by flame atomic absorption spectrophotometer. The amount of platinum measured was corrected by the amount of nickel measured.Limit of detection of platinum in the proposed method was 0.05 ppm and the calibration curve showed good linearity in the range from 0.1 to 2.0 ppm. The method is advantageous in reducing sample size and making highly sensitive analysis of p-atinum possible.Correction for the loss of p-atinum resulted from bumping or pretreatment of sample is also easy.The method will be helpful in determining platinum in pharmacological and pharmacokinetic studies of various antineoplastic platinum complexes. 続きを見る
6.

論文

論文
今成, 登志男 ; 大久保, 登 ; 早川, 和一 ; 宮崎, 元一
出版情報: 分析化学 = Japan analyst.  28  pp.285-288,  1979-01-01.  日本分析化学会 = The Japan Society for Analytical Chemistry
URL: http://hdl.handle.net/2297/44504
概要: 生体試料中の金属を電気透析試料調製器によって陽イオンとして直接抽出する方法(A法)とEDTA-金属錯体として陰イオンとして抽出する方法(B法)について条件の検討を行った. A法の条件は泳動液0.05N酢酸,電流密度15mA/cm2,抽出時間 (20~40)分,B法の条件は泳動液0.05N水酸化アンモニウム-1m M EDTA,電流密度20mA/cm2,抽出時問(20~40)分であった.A法では鉄(III),銅(II)の回収率80%,亜鉛(II)の回収率は90%,一方B法については鉄(III),亜鉛(II)の回収率100%,銅(II)の回収率は95%であった.たん白質として牛血清アルブミン,電解質として塩化ナトリウムの共存の影響を調べた後,B法を血しょう中のアルブミン結合亜鉛の分析に応用した. The extraction of metals from biological materials by an electrodialytic sample preparator was studied. Two procedures are proposed in this method : one depends on the extraction of metals as cationic ions (Mode A) and the other depends on the extraction of metal-EDTA complexes as anionic ions (Mode B). The basic conditions for Mode A are : carrier solution; 0.05 N CH3COOH, current density; 15 mA/cm2, extraction time; (2040) min. The conditions for Mode B are : carrier solution; 0.05 N NH4OH-1 mM EDTA, current density; 20 mA/cm2 and extraction time; (2040)min. The recoveries of Fe(III), Cu(II) and Zn(II) in Mode A were 90, 80 and 90%, and in Mode B the recoveries were 100, 95 and 100% respectively. The effects of electrolytes and proteins on the extraction of metals were examined, and the application of Mode B for the determination of albumin bound zinc in human plasma was carried out as follows : the supernatant containing albumin bound zinc was obtained by polyethylene glycol treatment and subjected to electrodialysis for 40 min according to the conditions described in Mode B. Albumin bound zinc in the extract was determined by atomic absorption spectrometry (AAS). Total plasma zinc was measured according to direct dilution method by AAS and α2-macroglobulin bound zinc was obtained by the difference. 続きを見る
7.

論文

論文
山本, 敦 ; 松永, 明信 ; 関口, 久義 ; 早川, 和一 ; 宮崎, 元一
出版情報: 衛生化学 = Eisei kagaku.  31  pp.47-50,  1985-01-01.  日本薬学会 = The Pharmaceutical Society of Japan
URL: http://hdl.handle.net/2297/44930
概要: Photometric ion chromatography is a novel method by using the photometer as a detector. A feature of this method is the use of light absorbing ion in the mobil phase, and the appearance of transparent sample ion in the effluent is detected by "trough"in the base line absorbance. We applied this technique by using a conventional HPLC system equipped with a commercially available ion exchange separation column to the determination of bromate in such foodstuffs as bread and kamaboko. Sample solution was prepared by aqueous extraction and mixed with acetone for removal of proteins. After evaporation of the organic solvent, the aqueous solution was dechlorinated by a DOWEX column (Ag-form), and then was subjected to chromatography. Samples of bread and kamaboko were spiked with 5.4 ppm and 8.4 ppm of bromate, respectively, and examined by the above method. The average recoveries were 88.3% in bread and 82.9% in kamaboko, and the coefficients of variation were 7.7% and 7.9%, respectively. The detection limit was 1 ppm. The above findings indicate that bromate can be detected by HPLC using conventional instrumentation, and the sensitivity of this system is comparable to conductimetric detection. 続きを見る
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論文

論文
早川, 和一 ; 山本, 敦 ; 宮崎, 元一
出版情報: 衛生化学 = Eisei kagaku.  40  pp.1-12,  1994-01-01.  日本薬学会 = The Pharmaceutical Society of Japan
URL: http://hdl.handle.net/2297/44934
概要: Ion chromatography (IC), which was introduced in 1975, has become widely used for the sensitive and simultaneous determination of ions. In the early stage, the IC system consisted of two ion-exchange columns (separator and suppressor, respectively) and a conductivity detector. Although this combination is still the most popular, new forms of separation and suppression have been developed. On the other hand, an indirect photometric detection using a UV detector has become popular in IC and other detectors commonly used in HPLC also have been used. Each of them has specific advantages for different types of analyses. Thus, IC is now defined as a form of liquid chromatography of ions. This article reviews new developments of the different forms of IC and their applications, especially to environmental samples, foods, biological materials and drugs, from reports published since 1990. 続きを見る
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論文

論文
吉田, 育世 ; 早川, 和一 ; 宮崎, 元一
出版情報: 衛生化学 = Eisei kagaku.  31  pp.317-323,  1985-01-01.  日本薬学会 = The Pharmaceutical Society of Japan
URL: http://hdl.handle.net/2297/44928
概要: Simultaneous determination of carboxylic acids and inorganic anions was investigated by photometric ion chromatography with phthalate solution as an eluent. The retention time and the peak area of carboxylic acids changed remarkably with eluent pH. The optimum conditions at present were as follows : analytical column, OYOBUNKO ASA-4000 (4.6 mm i.d.×25 cm) ; eluent, 5×10-4 M disodium phthalate ; flow rate, 2 ml/min ; column temperature, 40°C ; detection wavelength, 240 nm ; injection volume, 100 μl. The detection limits for several carboxylic acids as 3 times noise were 0.05-0.07 mg/l in sample solution. Their calibration curves showed straight lines by the peak area method. The present method has an advantage to determine simultaneously not only carboxylic acids but also inorganic anions. Under the condition described above, both carboxylic acids and inorganic anions in several liquid foods were easily determined by direct injection of the sample prepared with only dilution. 続きを見る
10.

論文

論文
木津, 良一 ; 東, 信一 ; 早川, 和一 ; 宮崎, 元一
出版情報: 分析化学 = Japan analyst.  35  pp.250-255,  1986-03-05.  The Japan Society for Analytical Chemistry = 日本分析化学会
URL: http://hdl.handle.net/2297/25011
概要: 金沢大学医薬保健研究域薬学系<br />血しょう及び尿中の制がん性白金錯体オキザラート(トランス-l-1,2-ジアミノシクロヘキサン)プラチニウム(II)(l-OHP)の分析法を研究した.種々検討した結果,分析条件は以下のように 定めた.前処理;Dowex 1-X8,Dowex 50 W-X4及びSep-Pak C_<18>カートリッジを一連のカラムとして構成する.この前処理カラムに血しょう及び尿の1mlと引き続き水を流す.前処理カラム溶出液の最初の6ml部分は捨て,次の10ml部分を分取し,高速液体クロマトグラフィー(HPLC)の試料とした.HPLC条件;カラム:Finepak SIL C_<18>(4.6mm i.d.×25cm),カラム温度:50℃,溶離液:水-メタノール(95:5),流速:1ml/min,検出波長:210nm,試料注入量:100μlピーク高さによる検量線は,原点を通る良い直線性を示した.定量下限は,血しょう及び尿中l-OHP濃度0.5μM,再現性はR.S.D.3%程度であった.血しょう及び尿中のl-OHPを高感度で精度良く定量することができた. A method for determination of (trans-l-1, 2-diaminocyclohexane) oxalatoplatinum (II) (l-OHP), introduced by Kidani et al. as one of the promising antineoplastic platinum complexes, in plasma and urine by high performance liquid chromatography (HPLC) was investigated in order to contribute for metabolic study of l-OHP. The recommended pretreatment procedures and HPLC conditions are as follows. Pretreatment of plasma and urine ; pretreatment column is composed of Dowex 1-X8 (5mm i. d.×6cm), Dowex 50W-X4 (5 mm i. d.×3 cm) and Sep-Pak C_<18> cartridge. Blood samples are centrifuged at 1000 g for 3 min to obtain plasma. One ml of plasma or urine is immediately placed on the pretreatment column and is eluted with water at the flow rate of 1.2 ml/min. The initial 6 ml portion of effluent is discarded and subsequent 10 ml portion is collected. An aliquot (100 μl) of the effluent collected is subjected to HPLC. All of these pretreatment procedures are carried out at 5±2℃. HPLC conditions ; column : Finepak SIL C_<18> (4.6 mm i. d.×25cm), eluent : H_2O-MeOH (95 : 5), column temperature : 40℃, flow rate : 1 ml/min, detection wavelength : 210 nm, injection volume : 100 μl. Under the conditions described above, a linear relationship was obtained between peak height and l-OHP concentration in plasma and urine. Determination limit was 0.5 μM l-OHP in sample solutions. Recovery of l-OHP in pretreatment was 86±3% in plasma and 83±2% in urine. A sensitive and precise determination of l-OHP in plasma and urine will be possible by this method. 続きを見る