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論文
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Imamura, S. ; Kobayashi, Junji ; Sakasegawa, S. ; Nohara, Atsushi ; Nakajima, Kenichi ; Kawashiri, Masa-aki ; Inazu, Akihiro ; Yamagishi, Masakazu ; Koizumi, Junji ; Mabuchi, Hiroshi ; 小林, 淳二 ; 野原, 淳 ; 中嶋, 憲一 ; 川尻, 剛照 ; 稲津, 明広 ; 山岸, 正和 ; 小泉, 順二 ; 馬渕, 宏
概要:
金沢大学医薬保健研究域医学系<br />The objective of this study was to establish a hepatic lipase (HL) assay method that can be applied
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to automatic clinical analyzers. Seventy-four hyperlipidemic subjects (men/women 45/29) were recruited. Lipase activity was assayed measuring the increase in absorbance at 546 nm due to quinonediimine dye production. Reaction mixture R-1 contained 50 mM Tris-HCl (pH 9.5), 0.5 mM glycerol-1,2-dioleate, 0.4% (unless otherwise noted) polyoxyethylenenonylphenylether, 3 mM ATP, 3 mM MgCl2, 1.5 mM CaCl2, monoacylglycerol-specific lipase, glycerol kinase, glycerol-3-phosphate oxidase, 0.075% N,N-bis-(4-sulfobutyl)-3-methylaniline-2 Na, peroxidase, ascorbic acid oxidase. Reaction mixture R-2 contained 50 mM Tris-HCl (pH9.5), 0.15% 4-aminoantypirine. Automated assay for activity was performed with a Model 7080 Hitachi analyzer. In the lipase assay, 160 μl of R-1 was incubated at 37°C with 3 μl of samples for 5 min, and 80 μl of R-2 was added. Within-run coefficient of variations was 0.9-1.0%. Calibration curve of lipase activity was linear (r = 0.999) between 0 and 320 U/l. Analytical recoveries of purified HL added to plasma were 96.6-99.8%. HL activity in postheparin plasma measured in this method had a closer correlation with HL mass by a sandwich ELISA (r = 0.888, P , 0.0001) than those in the conventional method using [ 14C-]triolein (r = 0.730, P < 0.0001). This assay method for HL activity can be applied to an automatic clinical analyzer. Copyright © 2007 by the American Society for Biochemistry and Molecular Biology, Inc.
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| 2.
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稲津, 明広 ; 小泉, 順二 ; Inazu, Akihiro ; Koizumi, Junji
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Kitatani, Masako ; Koizumi, Junji ; Inazu, Akihiro ; Kajinami, Kouji ; Mabuchi, Hiroshi ; 小泉, 順二 ; 稲津, 明広 ; 梶波, 康二 ; 馬渕, 宏
概要:
金沢大学医薬保健研究域保健学系<br />A study was conducted to determine the clinical efficacy and tolerability of simvastatin, an inhibi
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tor of 3-hydroxy-3-methylglutaryl coenzyme A reductase in seven patients with heterozygous familial hypercholesterolemia (FH) (defined as primary hypercholesterolemia with tendon xanthoma or primary hypercholesterolemia without tendon xanthomas and at least one first-degree relative with familial hypercholesterolemia). These patients were administrated 10 mg/d of simvastatin for up to 4 years. Simvastatin significantly reduced levels of both total cholesterol and low-density lipoprotein cholesterol during treatment; respective reduction rates were 28% and 34% after 1 month and 32% and 40% after 4 years. Serum high-density lipoprotein cholesterol levels slightly and insignificantly increased. Serum triglyseride levels fell significantly by 24% at month 6. After this study, all xanthomas had reduced in size. No adverse events related to simvastatin were observed. We conclude that long-term simvastatin therapy is clinically useful and well tolerated in patients with FH. Copyright © 1985 Published by Elsevier Inc.
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小泉, 順二
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宮崎, 節子 ; 田上, 芳美 ; 池田, 美智子 ; 亀田, 真紀 ; 伊藤, 大輔 ; 坪川, 俊成 ; 清水, 美保 ; 足立, 由美 ; 吉川, 弘明 ; 小泉, 順二
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第50回全国大学保健管理研究集会 ポスター資料
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小泉, 順二 ; Koizumi, Junji
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池上, 美智子 ; 田上, 芳美 ; 宮崎, 節子 ; 亀田, 真紀 ; 柿本, 晴美 ; 高, 紀子 ; 芦原, 百合子 ; 清水, 美保 ; 内山, 勝晴 ; 足立, 由美 ; 吉川, 弘明 ; 小泉, 順二
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第49回全国大学保健管理研究集会 ポスター資料
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小泉, 順二 ; Koizumi, Junji
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宮崎, 節子 ; 田上, 芳美 ; 池田, 美智子 ; 柿本, 晴美 ; 芦原, 百合子 ; 高, 紀子 ; 亀田, 真紀 ; 吉川, 弘明 ; 内山, 勝晴 ; 清水, 美保 ; 足立, 由美 ; 鈴木, 健一 ; 小泉, 順二
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第48回全国大学保健管理研究集会、ポスター資料 2010.10.20-21
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田上, 芳美 ; 宮崎, 節子 ; 池田, 美智子 ; 柿本, 晴美 ; 芦原, 百合子 ; 高, 紀子 ; 亀田, 真紀 ; 高信, 雅子 ; 生田, 聡美 ; 小畑, 公未子 ; 吉川, 弘明 ; 内山, 勝晴 ; 清水, 美保 ; 足立, 由美 ; 鈴木, 健一 ; 小泉, 順二
概要:
第48回全国大学保健管理研究集会、ポスター資料 2010.10.20-21
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