1.

論文

論文
早川, 和一 ; 平木, 博美 ; 宮崎, 元一
出版情報: 分析化学 = Japan analyst.  34  pp.71-76,  1985-07-05.  The Japan Society for Analytical Chemistry = 日本分析化学会
URL: http://hdl.handle.net/2297/24890
概要: 金沢大学医薬保健研究域薬学系<br />入手が容易なイオン交換カラムを備えた普及型HPLC装置で可能な,無機陰イオンの微量分析条件を検討した.無機陰イオン(炭酸イオン,塩化物イオン,亜硝酸イオン,リン酸イオン,臭化物イオン,硝酸 イオン,硫酸イオン,ヨウ化物イオン)は,フタル酸二ナトリウム溶液を溶離液として,陰イオン交換カラムで分離溶出された後,ベースラインの谷として紫外検出される.炭酸イオン,塩化物イオン,リン酸イオン,臭化物イオン,硝酸イオン,硫酸イオンの同時分析の最適条件は,カラム:Zipax SAX (2.1 mm i.d.×50cm):溶離液:5×10^<-4> Mフクル酸二ナトリウム;流速:1 ml/min; 温度:室温;検出波長:240 nm;注入量:100μlであった.この条件で,検出下限はppbレベルであり,ピーク高さ法と面積法のいずれでも検量線は直線となった.本法を用いると,環境試料中の主要無機陰イオンが短時間に一斉分析でき,しかも本法の分析値は,他法の結果ともよく一致した. A photometric determination method of microamounts of inorganic anions has been developed by conventional high performance liquid chromatography using a popular ion exchange column. Inorganic anions (HCO_3^-, Cl^-, NO_2^-, H_2PO_4^-, Br^-, NO_3^-, SO_4^<2->, and I^-) were separated on an anoin exchange column by using disodium phthalate solution as an eluent, and detected as troughs in the base line absorbance by a UV monitor. The optimum conditions for the determination of HCO_3^-, C1^-, H_2PO_4^-, Br^-, NO_3^-, and SO_4^<2-> were as follows : column, Zipax SAX (2.1 mm i.d. ×50cm) ; eluent, 5×10^<-4>M disodium phthalate ; flow rate, 1 ml/min ; column temperature, ambient ; detection wavelength, 240 nm. Under the conditions described above, detection limits of these anions were at levels as low as ppb. The calibration curves gave straight lines by either peak height or peak area method. Main inorganic anions contained in environmental samples could be simultaneously determined by the proposed method, and their analytical results were close to those obtained by other methods. 続きを見る
2.

論文

論文
早川, 和一 ; 平木, 博美 ; 宮崎, 元一
出版情報: 分析化学 = Japan analyst.  32  pp.504-505,  1983-08-05.  The Japan Society for Analytical Chemistry = 日本分析化学会
URL: http://hdl.handle.net/2297/24889
概要: 金沢大学医薬保健研究域薬学系<br />A novel method for the determination of micro amounts of inorganic ions has been developed. Th e separation of alkaline metal ions (Li^+, Na^+, and K^+) was accomplished on a Zipax SCX packed column (4.6 mm × 12 cm) with 2.5×10^<-4> M copper sulfate as an eluent, and the separation of halogen ions (F^-, Cl^-, and Br^-) was accomplished on a Zipax SAX packed column (4.6 mm × 15cm) with 1.0 × 10^<-3> M sodium benzoate as an eluent. The eluted ions were detected as troughs in base line absorbance of eluent ion at 220 nm or 240 nm. Under the conditions described above, the six ions were easily separated and determined at the levels as low as ppb by using either a peak height method or a peak area method which gave straight calibration curves. Other inorganic ions can be also determined by changing composition and concentration of the eluent. Moreover, this analytical method is economical and simple, since it can be performed with a conventional HPLC system equipped with a single separation column packed with inexpensive and easily available ion exchanger and a photometric detector. 続きを見る