※一部利用できない機能があります
| 1.
論文 |
論文
|
Hayakawa, Kazuichi ; Miyoshi, Yasuko ; Kurimoto, Hiroaki ; Matzushima, Yuko ; Takayama, Nariaki ; Tanaka, Seishi ; Miyazaki, Motoichi ; 早川, 和一 ; 三好, 裕子 ; 栗本, 博昭 ; 松島, 優子 ; 高山, 成明 ; 田中, 星司 ; 宮崎, 元一
概要:
金沢大学理工研究域自然システム学系<br />A HPLC determination method for methamphetamine (MA) and its metabolites in the urine samples of
…
abusers has been developed. MA, amphetamine (AP), norephedrine (NE), p-hydroxymethamphetamine (pOHMA), p-hydoroxyamphetamine (pOHAP) and an internal standard, namely β-phenylethylamine (PEA) were derivatized with dansyl chloride. They were separated on a reversed phase column with gradient elution using an acetonitrile/tetrahydrofuran/imidazole buffer mobile phase and chemilumigenically determined using bis(2, 4, 6-trichlorophenyl)-oxalate/hydrogen peroxide as post column reagents. The lower determination limits were as low as 1×10-14-3×10-14mol. AP, NE, pOHAP and PEA were derivatized with naphthalene-2, 3-dicarboxaldehyde, and were separated on a reversed phase column using an acetonitrile/imidazole buffer mobile phase and chemilumigenically determined. The lower determination limits were 3×10-16-1.5×10-15mol. Enzymatic hydrolysis of glucuronides of pOHMA (pOHMAG) and pOHAP (pOHAPG) allowed them to be determined as pOHMA and pOHAP, respectively. After adjusting the pH of the urine samples to 10.5 and adding PEA, all metabolites except glucuronides were extracted quantitatively into chloroform-isopropanol (3 : 1). Utilizing the two methods, MA and all metabolites were determined in urine samples of MA abusers. The tendency, in order of decreasing concentration was : [MA]>[A]>[pOHMAG]>[pOHMA]>[NE]>[pOHAPG]>[pOHAP]. Although ephedrine (EP) was detected in several samples, it was not considered to be a metabolite of MA but rather a component derived from cough medicine.
続きを見る
|
||||
| 2.
論文 |
論文
|
早川 , 和一 ; 能村 , 京子 ; 宮崎 , 元一 ; Hayakawa, Kazuichi ; Nomura, Kyoko ; Miyazaki, Motoichi
|
||||
| 3.
論文 |
論文
|
早川, 和一 ; 山本 , 敦 ; 宮崎, 元一 ; Hayakawa, Kazuichi ; Yamamoto, Atsushi ; Miyazaki, Motoichi
|
||||
| 4.
論文 |
論文
|
早川, 和一 ; 宮崎, 元一 ; Hayakawa, Kazuichi ; Miyazaki, Motoichi
|
||||
| 5.
論文 |
論文
|
Miyazaki, Motoichi ; Okubo, Noboru ; Hayakawa, Kazuichi ; Umeda, Toshihiko
概要:
A flow-injection analysis system is described for the determination of halide (chloride, bromide and iodide) anions. Thi
…
s method could be used as a post column detection method for these anions in water samples. The system is based on the specific and selective reaction between halide anion and fluorescein with an oxidant in an acidic flow. The product is detected at 530 nm in an alkaline flow. The detection limits of the three anions under the conditions tentatively established for the determination of bromide anion were as follows ; Cl-, 1×10-8mol ; Br-, 1×10-10mol ; I-, 1×10-10mol.
続きを見る
|
||||
| 6.
論文 |
論文
|
Imanari, Toshio ; Ishida, Yuko ; Hayakawa, Kazuichi ; Miyazaki, Motoichi
概要:
Superoxide dismutase (SOD)-like activity was found in rabbit plasma and the active substance was separated. Its properties were similar to those of copper-zinc SOD in cyanide and azide sensitivity and heat-stability, but its
…
molecular weight was presumed to be 140000 by Sephadex G-150 column.
続きを見る
|
||||
| 7.
論文 |
論文
|
Hayakawa, Kazuichi ; Nomura, Kyoko ; Miyazaki, Motoichi
|
||||
| 8.
論文 |
論文
|
Hayakawa, Kazuichi ; Kobayashi, Jun ; Ohmori, Mikako ; Ohya, Mayumi ; Kato, Akio ; Miyazaki, Motoichi
|
||||
| 9.
論文 |
論文
|
Murahashi, Tsuyoshi ; Miyazaki, Motoichi ; Kakizawa, Ryuichi ; Yamagishi, Yoshihisa ; Kitamura, Moritsugu ; Hayakawa, Kazuichi
概要:
Direct-acting mutagenic 1, 3-dinitropyrene (1, 3-DNP), 1, 6-DNP, 1, 8-DNP and 1-nitropyrene (1-NP) and indirect-acting mutagenic benzo [a] pyrene (BaP) in airborne particulates collected by the side of a busy intersection in downtown
…
Kanazawa were determined by high-performance liquid chromatography (HPLC) with chemiluminescence and fluorescence detection. Time courses of their concentrations were high in the morning (8 : 00-10 : 00) and evening (16 : 00-20 : 00) and low from the midnight until early morning (0 : 00-6 : 00). Levels of each DNP (in the range of fmol/m3) and 1-NP (in the range of sub pmol/m3) were, respectively, more than three and about one order of magnitude lower than that of BaP. Large correlation coefficients (0.85-0.91) between their concentrations, traffic volume, and carbon monoxide and nitrogen monoxide concentrations suggested that the main source was vehicles. Utilizing the concentration ratios, ([1, 3-DNP]+[1, 6-DNP]+[1, 8-DNP])/[1-NP], in airborne particulates (0.014), gasoline particulates (0.56) and diesel particulates (0.013), contributions (%) of dieselengine vehicles to the three DNPs and 1-NP in the air were estimated to be 94.3% and 99.8%, respectively.
続きを見る
|
||||
| 10.
論文 |
論文
|
Nishimura, Masayuki ; Hayashi, Morimasa ; Hayakawa, Kazuichi ; Miyazaki, Motoichi
概要:
The selective effect of triethylenetetramine (Trien) on the retention behavior of sulfate in ion chromatography was stud
…
ied. When Trien was added to an eluant as a counter ion, retention of sulfate was selectively shortened, while retention times of monovalent anions did not change. The cause for this phenomenon was the complexation of sulfate and Trien in an eluant. The stability constants of divalent sulfate-trivalent Trien and -tetravalent Trien complexes were estimated to be 936 and 4213 (40°C), respectively. As a practical application of this phenomenon, weakly retained monovalent species can be very well separated from both water dip and other species without prolonging the interval of injections.
続きを見る
|
