|
Genetics and embryology of the mouse: past, present, and future |
|
|
Summary of mouse development |
|
|
A mouse colony for the production of transgenic and chimeric animals |
|
|
Recovery and in vitro culture of preimplantation embryos |
|
|
Isolation, culture, and manipulation of postimplantation embryos |
|
|
Surgical procedures |
|
|
Production of transgenic mice by pronuclear microinjection |
|
|
Embryo-derived stem cell lines |
|
|
Germ line-competent stem cells derived from adult mice |
|
|
Vector designs for pluripotent stem cell-based transgenesis and genome alterations |
|
|
Introduction of foreign DNA into embryonic stem cells |
|
|
Production of chimeras |
|
|
Genotyping |
|
|
Parthenogenesis, pronuclear transfer, and mouse cloning |
|
|
Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection |
|
|
Cryopreservation, rederivation, and transport of mouse strains |
|
|
Techniques for visualizing gene products, cells, tissues, and organ systems |
|
|
Setting up a micromanipulation lab |
|
|
Genetics and embryology of the mouse: past, present, and future |
|
|
Summary of mouse development |
|
|
A mouse colony for the production of transgenic and chimeric animals |
|