1.

図書

図書
Charles Darwin
出版情報: New York : AMS Press, 1972
シリーズ名: The Works of Charles Darwin ; v. 10
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2.

図書

図書
Charles Darwin ; introduced by S. J. Rachman
出版情報: London : Julian Friedmann , New York : St. Martin's Press, 1979
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3.

図書

図書
edited by Angeliki Athanasiadou, Elżbieta Tabakowska
出版情報: Berlin ; New York : Mouton de Gruyter, 1998
シリーズ名: Cognitive linguistics research / editors René Dirven, Ronald W. Langacker ; 10
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4.

図書

図書
Paul Ekman
出版情報: New York : St. Martin's Griffin, c2007
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5.

図書

図書
Joel R. Davitz, with Michael Beldoch ... [et al.]
出版情報: New York : McGraw-Hill, c1964
シリーズ名: McGraw-Hill series in psychology
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6.

図書

図書
[by] Joel R. Davitz
出版情報: New York : Academic Press, 1969
シリーズ名: Personality and psychopathology ; v. 6
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7.

図書

図書
Maurice Merleau-Ponty ; texte établi et présenté par Claude Lefort
出版情報: Paris : Gallimard, c1969
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8.

図書

図書
Maurice Merleau-Ponty ; edited by Claude Lefort ; translated by John O'Neill
出版情報: Evanston : Northwestern University Press, c1973
シリーズ名: Northwestern University studies in phenomenology and existential philosophy
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9.

図書

図書
Sally Planalp
出版情報: Cambridge, UK ; New York : Cambridge University Press , Paris : Maison des Sciences de l'Homme, 1999
シリーズ名: Studies in emotion and social interaction ; . Second series
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10.

論文

論文
片岡, 邦重 ; Kataoka, Kunishige ; Tanaka, Kazuhiro ; Sakai, Yoko ; Sakurai, Takeshi
出版情報: Protein expression and purification.  41  pp.77-83,  2005-05-01.  Elsevier
URL: http://hdl.handle.net/2297/1732
概要: Bilirubin oxidase (BO) from Myrothecium verrucaria (authentic BO) catalyzing the oxidation of bilirubin to biliverdine was overexpressed in the methylotrophic yeast, Pichia pastoris. The cDNA encoding BO was cloned into the P. pastoris expression vector pPIC9K under the control of the alcohol oxidase 1 promoter and its protein product was secreted using the Saccharomyces cerevisiae α-mating factor signal sequence. The productivity of recombinant BO (rBO) in P. pastoris was approximately 5000 U/L of culture broth, being about 2.5- and 250-fold higher than rBO expressed in Aspergillus oryzae and S. cerevisiae, respectively. The calculated molecular mass of rBO consisting of 538 amino acids was 60,493 kDa, however, that of SDS–PAGE was 66 kDa because of non-native type N-linked sugar chains. The spectroscopic properties of rBO were typical of multicopper oxidase containing four Cu ions per protein molecule. The specific activity to oxidize bilirubin was 57 U/mg, having a value about twice that of authentic BO and rBO expressed in A. oryzae. Moreover, the thermostability of rBO expressed in P. pastoris was significantly high compared to the authentic BO previously reported. Accordingly, a heterologous expression system of rBO to meet clinical and industrial needs was constructed 続きを見る