※一部利用できない機能があります
| 1.
論文 |
論文
|
Wakayama, Tomohiko ; Koami, Hiroyuki ; Yamamoto, Miyuki ; Iseki, Shoichi
概要:
金沢大学大学院医学系研究科がん細胞学<br />Spermatogenic immunoglobulin superfamily (SgIGSF) is an adhesion molecule originally isolated fr
…
om adult mouse testis. In the testis, SgIGSF is expressed specifically in spermatogenic cells and may be involved in spermatogenesis. SgIGSF may also be involved in synapse formation and tumor suppression. In the present study, we examined the expression and cellular localization of SgIGSF in the entire adult mouse organs and tissues using Western blot analysis and immunohistochemistry at light and electron microscopic levels. Western blot analysis revealed that SgIGSF is expressed not only in the testis but also in the liver, lung, and nervous system including the cerebrum, cerebellum and sciatic nerve. The nervous system as well as testis showed multiple immunoreactive bands ranging from 45 to 100 kDa, whereas the liver and lung showed a single 100 kDa band. Immunohistochemisfry demonstrated that in the nervous system, SgIGSF is localized to the membranes of synapses, axons and Schwann cells. In contrast, in the lung and liver SgIGSF was localized to the membranes of apposing respiratory epithelial cells, hepatocytes and biliary epithelial cells. These results suggested that SgIGSF plays multiple physiological roles in the adult mouse as an adhesion molecule
続きを見る
|
||||
| 2.
論文 |
論文
|
水上, 勇治 ; Mizukami, Yuji
概要:
本研究ではmixed medullary-follicular carcinomaの組織発生を明らかにするために臨床病理学的,形態学的,生化学的検討を行った.結果は次の様であった.1.臨床病理学的には,本症は通常の髄様癌に比し,1).男性に
…
多い(6:1 vs 1:1).2).より若年者に多い(平均年齢36歳 vs 44歳).3).予后が良好である(5年生存率100% vs 78%).などの特徴がみられ,通常の髄様癌とは異なる臨床病理学的性格を示唆するものであった.2.髄様癌,mixed medullary-follicular carcinomaおよび種々の疾患の人甲状腺組織を用い,カルシトニン,サイログロブリンmRNA発現,蛋白発現をnorthern blot法,in situ hybridization法,免疫組織化学法を用い検討した.3.Northern blot法については甲状腺組織よりのRNA抽出に不備があり,このためカルシトニン,サイログロブリンmRNAの定量的観察が出来なかった.しかしin situ hybridization法,免役組織化学法についてはほぼ一致した結果が得られた.4.In situ hybridization法,免役組織化学法ともに,mixed medullary-follicular carcinomaでは髄様癌部にカルシトニンmRNA,蛋白共に発現しており,一方濾胞癌の部分ではサイログロブリンmRNA,蛋白共に発現していた.5.しかし,mixed medullary-follicular carcinomaの腫瘍細胞において,カルシトニン,サイログロブリンmRNA,蛋白は同一細胞において同時の発現は認められなかった.6.甲状腺髄様癌,甲状腺腺腫,乳頭癌等においても腫瘍細胞にカルシトニン,サイログロブリンmRNA,蛋白レベルとも同時発現は観察されなかった.<br />We studied to clarify the histogenesis of mixed medullary-follicular carcinoma of the thyroid using methods of clinicopathology, immunohistochemistry, in situ hybridization and northern blot. The results are as follows :1). Clinicopathologically, mixed medullary-follicular carcinomas are more frequently occurs compared with usual medullary carcinoma : in male patients (6 : 1 vs 1 : 1), in younger patients (36yrs vs 44yrs) and with better prognosis (100% vs 78% in 5-year survival).2). We failed to detection of calcitonin and thyroglobulin mRNA using northern blot, probably due to error of extraction of mRNA from thyroid tissues.3). Using in situ hybridization and immunohistochemistry, mRNA and protein of calcitonin or thyroglobulin are detected in mixed medullary-follicular carcinoma.4). We initially expected that mRNA or protein of calcitonin and thyroglobulin are concomitantly express in tumor cells of mixed medullary-follicular carcinoma. However, in the present study, such a concomitant expression of calcitonin and thyroglobulin in the same tumor cell was not observed.5). The concomitant expression of calcitonin and thyroglobulin was not observed in usual medullary carcinomas and other types of thyroid cancers.We will continue intensively the research for clarification of histogenesis of medullary-follicular carcinoma of thyroid.<br />研究課題/領域番号:10670156, 研究期間(年度):1998-1999<br />出典：「甲状腺髄様癌細胞におけるTHS受容体,TPO及びサイログロブリンmRNAの発現」研究成果報告書 課題番号10670156(KAKEN：科学研究費助成事業データベース（国立情報学研究所）) 本文データは著者版報告書より作成
続きを見る
|
