1.

論文

論文
Yamamoto, Atsushi ; Matsunaga, Akinobu ; Mizukami, Eiichi ; Hayakawa, Kazuichi ; Miyazaki, Motoichi
出版情報: 衛生化学.  36  pp.332-337,  1990-01-01.  日本薬学会 = The Pharmaceutical Society of Japan
URL: http://hdl.handle.net/2297/44929
概要: A simultaneous determination method for five organic acids in vinegar has been established by photometric ion chromatography. Both trace components (lactic, gluconic, succinic and pyroglutamic acids) and the major component (acetic acid) in vinegar were separated on a TSK gel IC-Anion-PW column with 0.45 mM o-aminobenzenesulfonic acid aqueous solution as an eluent. Their elutions were detected as positive peaks before the system peak, and a negative peak after that by a UV monitor. The sensitivities of trace components were enhanced by adjusting their elutions close to the system peak. The lowest detection limits were 10μM lactic acid, 5μM gluconic and succinic acids and 3μM pyroglutamic acid, respectively. The only pretreatment necessary was dilution of vinegar 100-fold with the eluent. The results obtained by the proposed method were in good agreement with those by the enzymatic one. 続きを見る
2.

論文

論文
山本, 敦 ; 松永, 明信 ; 牧野, 正雄 ; 早川, 和一 ; 宮崎, 元一
出版情報: 衛生化学 = Eisei kagaku.  33  pp.174-178,  1987-01-01.  日本薬学会 = The Pharmaceutical Society of Japan
URL: http://hdl.handle.net/2297/44937
概要: A method for the simultaneous determination of organic acids was investigated by photometric ion chromatography by using two different eluents. A 4.6 mm i.d.×25 cm stainless column packed with MCI GEL SCA 01 (Mitsubishi Chemical Ind., Ltd.) was used as a separator column. Sample was injected into this column previously equilibrated with 1 mM citraconic acid at pH 6.8. After elution of monobasic organic acids, the eluent was exchanged to a stronger one, 1 mM m-sulfobenzoic acid at pH 6.8. Dibasic organic acids were eluted before the column reequilibration with the secondary eluent. On the other hand, tribasic organic acids such as citric acid were eluted with the secondary eluent. To prevent the base line disorder due to the eluent exchange, the determination at 254 nm was performed. Because this wave length is an isosbestic point of the two eluents. Sour-taste agents in soft drinks could be analysed directly after dilution of samples. 続きを見る