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1.
論文 |
Deguchi, Mariko ; Kubota, N. ; Matsuno, A. ; Kanemori, Masaaki ; Fukumori, Yoshihiro ; Sasayama, Yuichi
概要:
Beard worms (Siboglinidae, Polychaeta) lack a mouth and a digestive tract and harbour chaemosynthetic bacteria in the bacteriocytes of the trophosome. Since beard worms depend on the organic compounds produced by the bacteria for
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nourishment, the bacteriocytes should be efficient in exchanging various substances with body fluids. For this reason, it is important to determine how the bacteriocytes are organized in the trophosome. As the first step of the present study, the appearance of bacteriocytes was examined in routinely stained paraffin sections. Second, visualization of the actual distribution of the bacteriocytes was attempted using whole-mount in situ hybridization with a probe of the 16S rRNA nucleotide sequence of the bacterium. After routine haematoxylin & eosin staining, the bacteriocytes appeared to be aligned in cell cords accompanied with nutrient-deposit cells that extended from both sides of the trophosome toward the dorsal side and folded up in the coelomic spaces. In whole-mount preparations, however, bacteriocytes with intense signals of 16S rRNA were seen three-dimensionally as many irregular leaves arranged from both sides of the ventral vessel toward the dorsal vessel. We will discuss the physiological significance of this characteristic distribution of the bacteriocytes in the present species. © 2007 The Authors Journal compilation © 2007 The Royal Swedish Academy of Sciences.
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2.
論文 |
Deguchi, Mariko ; Kubota, N. ; Matsuno, A. ; Kanemori, Masaaki ; Fukumori, Yoshihiro ; Sasayama, Yuichi
概要:
Beard worms (Siboglinidae, Polychaeta) lack a mouth and a digestive tract and harbour chaemosynthetic bacteria in the bacteriocytes of the trophosome. Since beard worms depend on the organic compounds produced by the bacteria for
…
nourishment, the bacteriocytes should be efficient in exchanging various substances with body fluids. For this reason, it is important to determine how the bacteriocytes are organized in the trophosome. As the first step of the present study, the appearance of bacteriocytes was examined in routinely stained paraffin sections. Second, visualization of the actual distribution of the bacteriocytes was attempted using whole-mount in situ hybridization with a probe of the 16S rRNA nucleotide sequence of the bacterium. After routine haematoxylin & eosin staining, the bacteriocytes appeared to be aligned in cell cords accompanied with nutrient-deposit cells that extended from both sides of the trophosome toward the dorsal side and folded up in the coelomic spaces. In whole-mount preparations, however, bacteriocytes with intense signals of 16S rRNA were seen three-dimensionally as many irregular leaves arranged from both sides of the ventral vessel toward the dorsal vessel. We will discuss the physiological significance of this characteristic distribution of the bacteriocytes in the present species. © 2007 The Authors Journal compilation © 2007 The Royal Swedish Academy of Sciences.
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3.
論文 |
神力, 甚一郎
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4.
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山崎, 豊
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5.
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北村, 圭三
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6.
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Nakagawa, Tomokiti
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7.
論文 |
Keimatsu, Mitsuo
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8.
論文 |
浅井, 喜久雄
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9.
論文 |
東, 正雄 ; 幸山, 彰一
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10.
論文 |
熊木, 義房
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